OriGene - pCMV6-A-GFP Destination Vector

Features:

This vector is essentially the same as the pCMV6-Entry vector, except for the alternative selection marker (GFP replacing Neomycin). GFP can be used to monitor transfection efficiency or as a cell sorting marker.
TurboGFP (excitation/ emission max = 482/ 502 nm) is mainly intended for applications where fast appearance of bright fluorescence is crucial. It is specially recommended for cell and organelle labeling and tracking the promoter activity. Structually it forms dimer. Learn more about TurboGFP
There is no drug selection marker in this vector for mammalian cells.
The vector itself does not contain additional tag. If the insert is shuttled from pCMV6-Entry via SgfI/AscI and RsrII/MluI, it will express non-tagged protein. If the insert is shuttled from the pCMV6-Entry vector using SgfI/AscI and Pme I, then it will express Myc-DDK tagged protein.

PrecisionShuttle mammalian vector with independent tGFP expression Vector Image

Schematic of the multiple cloning sites:

PrecisionShuttle mammalian vector with independent tGFP expression MCS Image

Download full vector sequence

OriGene's fluorescent protein genes are all licensed from Evrogen. Plasmid vectors harboring a fluorescent protein gene contains a proprietary nucleic acid coding for a proprietary fluorescent proteins(s) intended to be used for not-for-profit internal research purposes only, where "research purposes" means non-conmmercial uses or activities which (or the results of which) do not generate revenue. Any use of the proprietary nucleic acid or protein other than for research use is strictly prohibited. USE IN ANY OTHER APPLICATION REQUIRES A LICENSE FROM EVROGEN. To obtain such a license, please contact Evrogen at license@evrogen.com.

All OriGene’s TrueORFs inserts can be subcloned into this vector via custom cloning service. To use this service:

Find the gene of interest using any of the Gene Name, Gene Symbol, Accession Number or Keyword.

Search:
At the product page displaying the correct gene of interest, choose the appropriate destination vector and select the “Custom Cloning” .

PrecisionShuttle mammalian vector with independent tGFP expression