BIOPHYSICS AND COMPUTATIONAL BIOLOGY: Crystal structure of human aquaporin 4 at 1.8 Å and its mechanism of conductance, PNAS, May 2009; 106: 7437 - 7442. [AQP4]
Chordin-like 1 and Twisted Gastrulation 1 Regulate BMP Signaling following Kidney Injury, Barry W. Larman, Michele J. Karolak, Derek C. Adams, and Leif Oxburgh, J. Am. Soc. Nephrol., May 2009; 20: 1020 - 1031. [CHRDL1]
Competitive binding of musclin to natriuretic peptide receptor 3 with atrial natriuretic peptide, Shunbun Kita, Hitoshi Nishizawa, Yosuke Okuno, Masaki Tanaka, Atsutaka Yasui, Morihiro Matsuda, Yukio Yamada, and Iichiro Shimomura, J. Endocrinol., May 2009; 201: 287 - 295. [NPR1]
OriGene has two types available to our customers. All OriGene TrueClones are provided in a pCMV6 Cloning Vector and all are available for purchase as negative controls. pCMV6-XL4 PCMV6-XL5 PCMV6-XL6
In addition, OriGene is offering a Neomycin resistant version of its pCMV cloning vector as a kit to those customer who wish to create a stable cell line from our TrueClones pCMV6-NEO
All of the cDNAs within the TrueClone collection are cloned into a series of pCMV vectors, enabling uniform screening methods. The vector plasmid was originally obtained from the laboratory of Dr. David Russell. The full-length cDNA fragments are directionally inserted downstream from a eukaryotic transcriptional promoter capable of driving heterologous gene expression in a variety of mammalian cell lines and transgenic mice. The CMV promoter is covered under U.S. Patents 5,168,062 and 5,385,839 and its use is permitted for research purposes only. Any other use of the CMV promoter requires a license from the University of Iowa Research Foundation, 214 Technology Innovation Center, Iowa City, IA 52242. All three OriGene pCMV6 vectors were constructed with the same features and multiple cloning site (MCS); with the exception, that pCMV-XL6 has an SP6 transcriptional promoter instead of T7. The basic backbone of the vector series is presented diagrammatically below.
Key Functional Features of pCMV6-XL4, XL5 & XL6 vectors:
Vector size: 4.7kb
Selection marker in E. coli: Ampicillin-resistance
Selection marker in mammalian cells: None. For transient transfection only
Promoter for in vivo expression in mammalian cells: CMV promoter
Promoter for in vitro cell free system: T7 (for pCMV6-XL4 and pCMV6-XL5) and SP6 for (pCMV6-XL6)
Cloning sites: EcoRI and SalI. While EcoRI is still preserved, SalI is destroyed upon cloning.
Restriction sites for removing insert: NotI. *Two NotI sites are flanking the cloning sites in the vector.
Cell line suitable for transfection: COS, 293, Hela, CHO, NIH3T3, Mouse L cell, etc.
Transcription termination and polyadenylation signals: from human growth hormone (hGH) gene.
Extensive work has been done to engineer the vector to achieve high level of transgene expression level. When compared with another popular expression plasmid, pCDNA3.1 (Invitrogen), pCMV-based plasmids provide comparable if not higher level transgene expression.
Fig 1. Comparison of transgene expression level in pCMV6- and pCDNA3.1-based plasmids. CAT gene was cloned downstream of the promoters in the pCMV6 and pCDNA3.1 vectors. In three independent experiments, a same quantity of plasmid DNA was transfected into COS1 cell and the CAT activity was scored.
