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Over 1000 citations of OriGene cDNA clones

Silencing hsp25/hsp27 Gene Expression Augments Proteasome Activity and Increases CD8+ T-Cell–Mediated Tumor Killing and Memory Responses Cancer Prevention Research, Jan 2012; 5: 122 - 137. [HSPB1 ]

Activation of Androgen Receptor, Lipogenesis, and Oxidative Stress Converged by SREBP-1 Is Responsible for Regulating Growth and Progression of Prostate Cancer Cells Mol. Cancer Res., Jan 2012; 10: 133 - 142. [SREBF1 ]

Androgen Deprivation Causes Epithelial–Mesenchymal Transition in the Prostate: Implications for Androgen-Deprivation Therapy Cancer Res., Jan 2012; 72: 527 - 536. [AR ]

CB2 Cannabinoid Receptors Promote Neural Progenitor Cell Proliferation via mTORC1 Signaling J. Biol. Chem., Jan 2012; 287: 1198 - 1209. [Cnr2 ]

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AKT1 (NM_005163) Human Kinase Deficient Mutant

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SC323430

NM_005163

Kinase deficient mutant (K179M) of Homo sapiens v-akt murine thymoma viral oncogene homolog 1 (AKT1), transcript variant 1 as transfection-ready DNA NM_005163.2

10ug

$680

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The use of this clone has been cited in the linked publication

Click here for the corresponding wild type clone.

OriGene TrueORF Data

Vector:	pCMV6-XL5	Insert Size: 2380	Restriction Site:
Sequence Data:	Fully Sequenced ORF 5' Read Nucleotide Sequence Kinase domain sequence
OTI Annotation:	This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.
OTI Disclaimer:	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components:	The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Protein Families:	Protein Kinase ES Cell Differentiation/IPS Druggable Genome
Protein Pathways:	MAPK signaling pathway ErbB signaling pathway Chemokine signaling pathway mTOR signaling pathway Apoptosis VEGF signaling pathway More Pathways >>

Reference Data

RefSeq: NM_005163.2, NP_005154	RefSeq Size: 3008	RefSeq ORF: 1443
Synonyms : AKT; MGC99656; PKB; PKB-ALPHA; PRKBA; RAC; RAC-ALPHA
LocusID: 207	Cytogenetic: 14q32.32	Domains: pkinase, pkinase_C, TyrKc, PH, S_TKc
Summary: The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq]. Transcript Variant: This variant (1) is the longest transcript. Variants 1, 2 and 3 encode the same protein.

Over 1000 citations of OriGene cDNA clones

AKT1 (NM_005163) Human Kinase Deficient Mutant

Click here to learn about OriGene’s recombinant AKT1 protein