OriGene - PFKL (NM_001002021) cDNA Clone

OriGene Data

Vector:	pCMV6-XL5	Insert Size:	Restriction Site:
Sequence Data:	Fully Sequenced ORF 5' Read Nucleotide Sequence
OTI Disclaimer:	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components:	The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Protein Families:	Druggable Genome
Protein Pathways:	Glycolysis / Gluconeogenesis Pentose phosphate pathway Fructose and mannose metabolism Galactose metabolism Metabolic pathways

Reference Data

RefSeq: NM_001002021.1, NP_001002021	RefSeq Size: 3402	RefSeq ORF: 2484
Synonyms : PFK-B, FLJ30173, FLJ40909, DKFZp686G1648, DKFZp686L2097
LocusID: 5211	Cytogenetic: 21q22.3
Summary: Phosphofructokinase (PFK) is a tetrameric enzyme that catalyzes a key step in glycolysis, namely the conversion of D-fructose 6-phosphate to D-fructose 1,6-bisphosphate. Separate genes encode a muscle subunit (M) and a liver subunit (L). PFK from muscle is a homotetramer of M subunits, PFK from liver is a homotetramer of L-subunits, while PFK from platelets can be composed of any tetrameric combination of M and L subunits. The protein encoded by this gene represents the L subunit. Alternate splicing results in two transcript variants, one of which is a candidate for nonsense-mediated decay (NMD). [provided by RefSeq, Aug 2008]. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (a).

TrueClone Citations:

Extreme C-terminal sites are posttranslocationally glycosylated by the STT3B isoform of the OST, Shiteshu Shrimal, Steven F. Trueman, and Reid Gilmore, J. Cell Biol., Apr 2013; 201: 81 - 95. [CD40]

Cellular glutathione plays a key role in copper uptake mediated by human copper transporter 1, Edward B. Maryon, Shannon A. Molloy, and Jack H. Kaplan, Am J Physiol Cell Physiol, Apr 2013; 304: C768 - C779. [CSS]

B-cell Maturation Antigen Is a Promising Target for Adoptive T-cell Therapy of Multiple Myeloma, Robert O. Carpenter, Moses O. Evbuomwan, Stefania Pittaluga, Jeremy J. Rose, Mark Raffeld, Shicheng Yang, Ronald E. Gress, Frances T. Hakim, and James N. Kochenderfer, Clin. Cancer Res., Apr 2013; 19: 2048 - 2060. [BCMA ]

Retinoic Acid Receptor ß Stimulates Hepatic Induction of Fibroblast Growth Factor 21 to Promote Fatty Acid Oxidation and Control Whole-body Energy Homeostasis in Mice, Yu Li, Kimberly Wong, Kenneth Walsh, Bin Gao, and Mengwei Zang, J. Biol. Chem., Apr 2013; 288: 10490 - 10504. [FGF21 ]

Natriuretic Peptide Receptor-3 Gene (NPR3): Nonsynonymous Polymorphism Results in Significant Reduction in Protein Expression Because of Accelerated Degradation, Naveen L. Pereira, Dong Lin, Linda Pelleymounter, Irene Moon, Gail Stilling, Bruce W. Eckloff, Eric D. Wieben, Margaret M. Redfield, John C. Burnett, Jr, Vivien C. Yee, and Richard M. Weinshilboum, Circ Cardiovasc Genet, Apr 2013; 6: 201 - 210. [NPR3]

A Modified Form of Diphthamide Causes Immunotoxin Resistance in a Lymphoma Cell Line with a Deletion of the WDR85 Gene, Hui Wei, Tapan K. Bera, Alan S. Wayne, Laiman Xiang, Simona Colantonio, Oleg Chertov, and Ira Pastan, J. Biol. Chem., Apr 2013; 288: 12305 - 12312. [WDR85]

Arachidonic Acid–Induced Dilation in Human Coronary Arterioles: Convergence of Signaling Mechanisms on Endothelial TRPV4-Mediated Ca2+ Entry, Xiaodong Zheng, Natalya S. Zinkevich, Debebe Gebremedhin, Kathryn M. Gauthier, Yoshinori Nishijima, Juan Fang, David A. Wilcox, William B. Campbell, David D. Gutterman, and David X. Zhang, JAHA, Apr 2013; 2: e000080. [TRPV4 ]

Cytoglobin has bimodal: tumour suppressor and oncogene functions in lung cancer cell lines, Urszula Oleksiewicz, Triantafillos Liloglou, Kalliopi-Maria Tasopoulou, Nikoleta Daskoulidou, Julie Bryan, John R. Gosney, John K. Field, and George Xinarianos, Hum. Mol. Genet., Apr 2013; 10.1093/hmg/ddt174. [CYGB ]

Comparative Phosphoproteomic Analysis of Checkpoint Recovery Identifies New Regulators of the DNA Damage Response, Vincentius A. Halim, Mónica Alvarez-Fernández, Yan Juan Xu, Melinda Aprelia, Henk W. P. van den Toorn, Albert J. R. Heck, Shabaz Mohammed, and René H. Medema, Sci. Signal., Apr 2013; 6: rs9. [SPAG5]

More TrueClone Citations >>

PFKL Mouse Clones

SKU	Description	Amount	Price	Shipping
MC203910	Mus musculus phosphofructokinase, liver, B-type (Pfkl) as transfection-ready DNA	10 ug	$165	Next day
MR210614	Myc-DDK-tagged ORF clone of Mus musculus phosphofructokinase, liver, B-type (Pfkl) as transfection-ready DNA	10 ug	$940	Next day
MG210614	GFP-tagged ORF clone of Mus musculus phosphofructokinase, liver, B-type (Pfkl) in a GFP expression vector	10 ug	$1030	3 weeks

PFKL Rat Clones

SKU	Description	Amount	Price	Shipping
RN210824	NativeORF clone of Rattus norvegicus phosphofructokinase, liver (Pfkl) as transfection-ready DNA	10 ug	$937	6 weeks
RR210824	Myc-DDK-tagged ORF clone of Rattus norvegicus phosphofructokinase, liver (Pfkl) as transfection-ready DNA	10 ug	$937	6 weeks

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PFKL (NM_001002021) Human cDNA Clone

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PFKL Mouse Clones

PFKL Rat Clones

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