Transfection Reagents

OriGene provides a broad range of transfection reagents designed to meet your specific cDNA transfection needs. 

• MegaTran 1.0 is a brandnew polymer based transfection reagent specially designed and manufactured for large volume DNA transfection, including large scale protein production via transient transfection, high-throughput screening using cDNA arrays or shRNA libraries, etc.
  • Superior transfection efficiency
  • Low toxicity
  • Improved protein production via transient transfection
  • Reduced cytotoxicity
  • Extremely affordable, especially for large volumn uses
    

• TurboFectin 8.0™ is a new generation of transfection reagent optimized for nucleic acid delivery into eukaryotic cells. Its proprietary formulation of lipid/histone blend is supplied in 80% alcohol. TurboFectin 8.0 is the recommended transfection reagent for delivery of TrueClone  (for over-expression) and HuSH-29 constructs (shRNA for expression knock-down) in many different cell types. TurboFectin 8.0 has also been optimized and is the preferred reverse transfection reagent in GFC-Transfection Arrays.

  • High efficiency: Comparable with the leading brand FuGENE^®6
  • Low toxicity: Comparable with the leading brand FuGENE^®6
  • Simplified use: Works well in media containing antibiotic and antimycotic agents. Suitable for serum-containing media; no requirement for media changes.
  • Wide spectrum: Over 100 cell lines and primary cell types have been successfully transfected with TurboFectin 8.0 (Click for list)
  • Affordability: One mL of TurboFectin 8.0 can typically be used for 500 transfections of 6-well plate cultures. The cost is less than $0.50 per transfection
   
• Magnetofection™ - A novel, simple and highly efficient method to transfect cells in culture. It exploits magnetic force exerted upon gene vectors associated with magnetic particles to draw the vectors towards, possibly even into, the target cells. In this manner, the full vector dose applied gets concentrated on the cells within a few minutes so that 100% of the cells get in contact with a significant vector dose. This has several important consequences: 
  • Validated to work in a broad range of cell types
  • Greatly improved transfection rates in terms of percentage of cells transfected compared to standard transfectiom
  • Up to several thousand fold increased levels of transgene expression compared to standard transfections upon short-term incubation.
  • High transfection rates and transgene expression levels are achievable with extremely low vector doses, which allows to save expensive transfection reagents.
  • Extremely short process time. A few minutes of incubation of cells with gene vectors are sufficient to generate high transfection efficiency, compared to several hours with standard procedures

• Magnetofection Reagent Only Available in the US