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OriGene cDNA clones in recent publications
Expression and Function of Epithelial Anoctamins J. Biol. Chem., Mar 2010; 285: 7838 - 7845 [ANO10]

Retinoid-suppressed phosphorylation of RARa mediates the differentiation pathway of osteosarcoma cells Oncogene (1 March 2010) doi:10.1038/onc.2010.50 Original Article [RARA]

A chemical and phosphoproteomic characterization of dasatinib action in lung cancer Nature Chemical Biology (28 February 2010) doi:10.1038/nchembio.332 Article [PTK6]

A Comparison of the Roles of Peroxisome Proliferator-Activated Receptor and Retinoic Acid Receptor on CYP26 Regulation Mol. Pharmacol., Feb 2010; 77: 218 - 227 [CYP26B1]

View All Citations >>

Transfection Reagents

A broad range of transfection reagents to meet your specific cDNA and shRNA transfection needs.

Cell Types Examples Recommended Reagents
Commonly used established cell lines Hela, MCF 7, K562, Jurkat, 3T3, COS TurboFectin 8.0
Most established cell lines and primary cells Above, plus MEF, HUVEC, SMC, lymphocytes, etc. Magnetofection PolyMag
Magnetofection CombiMag
Primary neurons and neuronal cell lines Hippocampal neurons and neuronal cell lines PC-12, B95, C6, N2A or SH-5YSY, etc. Magnetofection NeuroMag
Virus infection of non-permissive cells   Magnetofection ViroMag
HEK293T, CHO, 293S, etc   MegaTran 1.0
  • TurboFectin 8.0is a new generation of transfection reagent optimized for nucleic acid delivery into eukaryotic cells. Its proprietary formulation of lipid/histone blend is supplied in 80% alcohol. TurboFectin 8.0 is the recommended transfection reagent for delivery of TrueClone/TrueORF Clone (for over-expression) and HuSH-29 constructs (shRNA for expression knock-down) in many established cell lines and cell types.
    • Superior performance: Comparable with FuGENE®6 in transfection efficiency and low cytotoxicity
    • Wide spectrum: Over 100 cell lines and primary cell types have been successfully transfected with TurboFectin 8.0
    • Affordability: cost lower than FuGENE®
  • Magnetofection - A novel and highly efficient method to transfect cells in culture. It exploits magnetic force exerted upon gene vectors associated with magnetic particles to draw the vectors into target cells.
    • Validated to work in a broad range of cell types, including primary cells and neuronal cells
    • Greatly improved transfection rates compared to standard transfection
    • Up to several thousand fold increased levels of transgene expression compared to standard transfections upon short-term incubation
    Learn more about our Spring Special Promotion
    Magnetofection Reagents are only available through OriGene in the US
  • MegaTran 1.0 is a polymer based transfection reagent specially designed and manufactured for high volume DNA transfection and large scale protein production in HEK293T, CHO and 293S cells.
    • Superior transfection efficiency and low toxicity
    • Improved protein production via transient transfection
    • Extremely affordable

 


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