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GPCR CLONESET
G protein-coupled receptors (GPCRs) form a large superfamily of membrane proteins that modulate sensory perception, chemotaxis, neurotransmission, cell communication, and many other vital physiological events. Characterized by their cell-surface localization and tissue-specificity, these protein receptors are the targets of 50-60% of all existing medicines including well-known -blockers and anti-histamine therapeutics. It is generally accepted that a better understanding of the function of these receptors and their structure will help in the design of drugs for the treatment of GPCR-related diseases. It is estimated that 340-400 pharmaceutically relevant GPCRs exist in the human genome, although many have been classified as orphan receptors because their endogenous ligands have not yet been identified. GPCR are generally classified into three groups: Rhodopsins, Secretins, and Metabolic Glutamate Receptors. The Rhodopsins include hormone, neurotransmitter and light receptors. The Secretins include calcitonin, parathyroid hormone, and vasoactive intestinal peptide. The Metabotropic Glutamate Receptors are similar in signature to calcium-sensing and GABA receptors. The OriGene GPCR CloneSet consists of 340 full-length human non-olfactory G Protein-Coupled Receptor genes. All genes in the GPCR CloneSet are cloned in expression vectors directly downstream of a CMV promoter and are ready for protein expression in mammalian cells. Each clone has been sequenced from the 5 and 3 ends to infer the inclusion of the initiation and stop codons. For those genes sharing multiple splicing variants at the same locus, the variable regions are sequence-verified to qualify a clone to a particular reference. In some cases, OriGene identified variants different than those described in the public database. With no public reference available, such sequences are annotated with a reference number from the same locus, and marked with an asterisk *. The comprehensive nature of the GPCR CloneSet and the uniformity and expression-readiness of the cloning vector enables a system biology approach to drug-potency, drug-toxicity and ligand identification studies of this pharmaceutically important gene family.
Download GPCR CloneSet Fact Sheet
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