World's Largest Collection of Full-length cDNA Clones 

There are two major classes of expression-ready clones offered by OriGene:

• TrueORF Clone:  Tagged Open Reading Frame (ORF) clones in a PrecisionShuttle vector. Ideal for over-expression of tagged proteins.


• TrueClone:  Authentic full-length cDNA clones containing native 5' and 3' untranslated region. Ideal for over-expression of the native proteins.

New: The standard shipement is 10ug transfection-ready purified plasmid DNA. Starting Aug 4th, 2008, options for additional quantities are available at a norminal fee. 20 ug: $50 extra; 50 ug: $150 extra; 100 ug: $250 extra. For 50 and 100 ug quantities, delivery may take up to 4 additional weeks.

Clone Brochure                 Application Guide                      FAQs

Why do thousands of scientists choose to use TrueClone™? 

TrueClones are available as 10 ug of purified plasmid DNA derived from a single colony, and end-sequence verified before shipment.  This format provides unmatched convenience and industry-leading accuracy and saves at least 3 days of routine lab work and related costs. 

Most clones from other providers are provided as bacterial stocks that need to be converted to purified plasmid for identity verification and for downstream applications, such as transfection, subcloning, protein expression in cell-free systems, etc.  You are then asked to verify that you received the correct stock. 

OriGene realizes that such quality control should not be your job. We put in extra effort to deliver pure plasmids so that you can start your project on the first day the clone arrives.

Experience this TrueClone Advantage today for yourself.  Search for a gene.

TrueClone Advantages:

TrueClones are a cost-effective and time-saving alternative to de novo cloning.
TrueClones are expression-ready and transfection-ready.
The TrueClone collection contains the largest number of full-length transcripts
TrueClones are authentic cDNA clones without PCR errors
TrueClones have consistent vectors
TrueClones are accurately prepared and delivered

1.  TrueClone is a cost-effective and time-saving alternative to de novo cloning.  Although some believe that gene cloning has become molecular biology 101, this still requires substantial resources and time to clone even the most common gene into an expression vector.  It should be pointed out that all of the easy-to-clone transcripts have been isolated, and the remaining are rare, large, GC-rich, or toxic to your standard cloning bacteria.  Current cloning estimates of time and effort required are usually grossly underestimated. 

Why spend your time, resources and energy on gene cloning when a pre-cloned gene is available and ready to be shipped to your laboratory?  Approximately half of the collection is offered at $245 for 10ug of each purified plasmid DNA.

2.  TrueClones are expression-ready and transfection-ready.  All TrueClones have a strong CMV promoter upstream of the cDNA for mammalian expression.  The plasmid can be transfected immediately for protein over-expression.  The images below are a few samples showing the validated over-expression of several popular TrueClones in HEK293 cells.

3. The TrueClone collection contains the largest number of full-length transcripts compared to other clone providers.  Transcripts from over 98% of the human genome are offered. 
When you need a clone, you have the best chance of obtaining it from OriGene.  While others provide the common clones, the Trueclone collection contains thousands of difficult-to-clone transcripts that are only available from OriGene.  OriGene’s dedication and technological R&D effort separates TrueClone from the rest of world.  Recently another 800 difficult-to-clone cDNAs were added to the TrueClone collection.  (click to see list).

4. Authenticity.  Each TrueClone is derived from cDNA library instead of PCR amplification, representing a real transcript without PCR-introduced artifacts.  Otherwise, your downstream application may be affected by unintended mutations.

5. Consistent vectors. While other vendors provide clones in a conglomeration of vectors with various antibiotic selection markers and variable expression-readiness, all TrueClones are in a single series of CMV vectors.  Every TrueClone is ready for immediate protein over-expression in mammalian cells or in cell-free systems utilizing T7 or SP6 promoters. 
A TrueClone’s expression-readiness and its uniform vector system render it ideal for high-throughput screening for functional genes.  Researchers from Novartis and Harvard have pioneered such novel applications using over 10,000 TrueClones and have made several successful discoveries.  (References)

6. Accuracy.  Verified, pure plasmid DNA stocks will be delivered instead of bacterial stocks.  No need for colony screening, plasmid preparation and restriction digestion/sequencing, which are all necessary when ordering a clone from most suppliers.

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