Human OGT activation kit by CRISPRa

CAT#: GA105599

OGT CRISPRa kit - CRISPR gene activation of human O-linked N-acetylglucosamine (GlcNAc) transferase

Product Images

Specifications

Product Data

• Format: 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug)
• Symbol: OGT
• Locus ID: 8473
• Kit Components:

  GA105599G1, OGT gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA105599G2, OGT gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA105599G3, OGT gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPRa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_003605, NM_181672, NM_181673
• UniProt ID: O15294
• Synonyms: FLJ23071; HRNT1; MGC22921; O-GLCNAC
• Summary: This gene encodes a glycosyltransferase that catalyzes the addition of a single N-acetylglucosamine in O-glycosidic linkage to serine or threonine residues. Since both phosphorylation and glycosylation compete for similar serine or threonine residues, the two processes may compete for sites, or they may alter the substrate specificity of nearby sites by steric or electrostatic effects. The protein contains multiple tetratricopeptide repeats that are required for optimal recognition of substrates. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Oct 2009]