Human MADH7 (SMAD7) activation kit by CRISPRa

CAT#: GA102780

SMAD7 CRISPRa kit - CRISPR gene activation of human SMAD family member 7

Product Images

Specifications

Product Data

• Format: 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug)
• Symbol: SMAD7
• Locus ID: 4092
• Kit Components:

  GA102780G1, MADH7 gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA102780G2, MADH7 gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA102780G3, MADH7 gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPRa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_001190821, NM_001190822, NM_001190823, NM_005904
• UniProt ID: O15105
• Synonyms: CRCS3; MADH7; MADH8
• Summary: The protein encoded by this gene is a nuclear protein that binds the E3 ubiquitin ligase SMURF2. Upon binding, this complex translocates to the cytoplasm, where it interacts with TGF-beta receptor type-1 (TGFBR1), leading to the degradation of both the encoded protein and TGFBR1. Expression of this gene is induced by TGFBR1. Variations in this gene are a cause of susceptibility to colorectal cancer type 3 (CRCS3). Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2010]