L2HGDH (NM_024884) Human Untagged Clone

CAT#: SC305181

L2HGDH (untagged)-Human L-2-hydroxyglutarate dehydrogenase (L2HGDH), nuclear gene encoding mitochondrial protein

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: L2HGDH
• Synonyms: C14orf160; L2HGA
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC305181 representing NM_024884.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGTGCCAGCGCTGCGTTATTTGGTTGGTGCCTGCGGACGGGCCCGCGGGCTTTTCGCCGGTGGCTCC
  CCTGGGGCGTGCGGGTTCGCGTCTGGGAGGCCAAGACCGCTGTGTGGAGGTAGCCGCAGCGCCAGCACC
  AGCTCATTTGATATAGTCATCGTTGGTGGCGGAATTGTGGGGCTTGCCTCTGCCAGAGCACTCATCCTG
  CGACATCCATCACTTTCTATTGGTGTTCTGGAAAAGGAGAAAGATTTAGCTGTTCACCAGACTGGACAT
  AACAGTGGTGTCATACATAGTGGAATTTATTATAAACCTGAGTCTCTGAAAGCCAAATTATGTGTACAA
  GGTGCAGCCCTCCTCTATGAGTACTGTCAGCAAAAGGGAATTTCCTACAAGCAGTGTGGCAAGCTTATA
  GTAGCTGTTGAACAAGAAGAAATTCCCAGACTTCAGGCCCTATATGAGAAAGGCCTCCAGAATGGTGTC
  CCGGGCCTGAGGCTGATCCAGCAGGAGGATATAAAAAAGAAGGAGCCATATTGTAGGGGTCTAATGGCT
  ATTGATTGTCCACATACTGGCATTGTGGACTATCGGCAGGTGGCTTTGTCATTTGCCCAGGATTTCCAA
  GAAGCAGGTGGCTCTGTCTTGACCAATTTTGAAGTAAAAGGTATTGAAATGGCTAAAGAAAGTCCTTCA
  AGAAGTATAGATGGAATGCAATATCCAATTGTTATAAAGAATACAAAGGGAGAGGAAATTCGATGTCAG
  TATGTTGTGACATGTGCAGGACTTTACTCAGACCGTATTTCAGAGTTGAGTGGCTGCACTCCTGATCCT
  CGAATTGTACCATTCCGGGGAGATTACCTGCTTTTGAAGCCAGAAAAATGTTATCTTGTAAAAGGAAAT
  ATTTATCCGGTCCCAGATAGCCGGTTTCCTTTCCTAGGAGTTCACTTCACACCAAGGATGGATGGCAGT
  ATTTGGCTAGGGCCTAATGCAGTTCTTGCCTTTAAACGAGAGGGTTACAGACCCTTTGACTTCAGTGCC
  ACAGATGTTATGGATATAATTATCAATAGTGGCTTGATTAAACTGGCATCCCAGAATTTTTCCTATGGA
  GTTACTGAAATGTATAAAGCATGTTTTCTTGGTGCAACAGTGAAGTATCTTCAAAAATTCATCCCTGAA
  ATTACTATCAGTGATATACTTAGGGGCCCAGCTGGAGTAAGAGCCCAGGCCCTGGATAGAGATGGAAAT
  CTGGTAGAAGATTTTGTATTTGATGCAGGAGTTGGGGATATTGGAAATCGCATTCTTCATGTGAGAAAT
  GCACCTTCTCCTGCTGCTACTTCTTCCATTGCAATTTCTGGAATGATTGCAGATGAAGTACAACAAAGA
  TTTGAATTATAA
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_024884
• Insert Size: 1392 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_024884.2
• RefSeq Size: 6111 bp
• RefSeq ORF: 1392 bp
• Locus ID: 79944
• UniProt ID: Q9H9P8
• Cytogenetics: 14q21.3
• Protein Pathways: Butanoate metabolism
• MW: 50.3 kDa
• Gene Summary: This gene encodes L-2-hydroxyglutarate dehydrogenase, a FAD-dependent enzyme that oxidizes L-2-hydroxyglutarate to alpha-ketoglutarate in a variety of mammalian tissues. Mutations in this gene cause L-2-hydroxyglutaric aciduria, a rare autosomal recessive neurometabolic disorder resulting in moderate to severe cognitive disability. [provided by RefSeq, Jul 2008]