CD147 (BSG) (NM_001728) Human Untagged Clone

CAT#: SC303059

BSG (untagged)-Human basigin (Ok blood group) (BSG), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CD147
• Synonyms: 5F7; CD147; EMMPRIN; EMPRIN; HAb18G; OK; SLC7A11; TCSF
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_001728 edited
  ATGGCGGCTGCGCTGTTCGTGCTGCTGGGATTCGCGCTGCTGGGCACCCACGGAGCCTCC
  GGGGCTGCCGGCTTCGTCCAGGCGCCGCTGTCCCAGCAGAGGTGGGTGGGGGGCAGTGTG
  GAGCTGCACTGCGAGGCCGTGGGCAGCCCGGTGCCCGAGATCCAGTGGTGGTTTGAAGGG
  CAGGGTCCCAACGACACCTGCTCCCAGCTCTGGGACGGCGCCCGGCTGGACCGCGTCCAC
  ATCCACGCCACCTACCACCAGCACGCGGCCAGCACCATCTCCATCGACACGCTCGTGGAG
  GAGGACACGGGCACTTACGAGTGCCGGGCCAGCAACGACCCGGATCGCAACCACCTGACC
  CGGGCGCCCAGGGTCAAGTGGGTCCGCGCCCAGGCAGTCGTGCTAGTCCTGGAACCCGGC
  ACAGTCTTCACTACCGTAGAAGACCTTGGCTCCAAGATACTCCTCACCTGCTCCTTGAAT
  GACAGCGCCACAGAGGTCACAGGGCACCGCTGGCTGAAGGGGGGCGTGGTGCTGAAGGAG
  GACGCGCTGCCCGGCCAGAAAACGGAGTTCAAGGTGGACTCCGACGACCAGTGGGGAGAG
  TACTCCTGCGTCTTCCTCCCCGAGCCCATGGGCACGGCCAACATCCAGCTCCACGGGCCT
  CCCAGAGTGAAGGCTGTGAAGTCGTCAGAACACATCAACGAGGGGGAGACGGCCATGCTG
  GTCTGCAAGTCAGAGTCCGTGCCACCTGTCACTGACTGGGCCTGGTACAAGATCACTGAC
  TCTGAGGACAAGGCCCTCATGAACGGCTCCGAGAGCAGGTTCTTCGTGAGTTCCTCGCAG
  GGCCGGTCAGAGCTACACATTGAGAACCTGAACATGGAGGCCGACCCCGGCCAGTACCGG
  TGCAACGGCACCAGCTCCAAGGGCTCCGACCAGGCCATCATCACGCTCCGCGTGCGCAGC
  CACCTGGCCGCCCTCTGGCCCTTCCTGGGCATCGTGGCTGAGGTGCTGGTGCTGGTCACC
  ATCATCTTCATCTACGAGAAGCGCCGGAAGCCCGAGGACGTCCTGGATGATGACGACGCC
  GGCTCTGCACCCCTGAAGAGCAGCGGGCAGCACCAGAATGACAAAGGCAAGAACGTCCGC
  CAGAGGAACTCTTCCTGA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001728 unedited
  GAGGATTGTATACGACTCCTATAGGGCGGCCGCGTATCCTCATGGCGGCCTGCGCTGTTC
  GTGCTGCTGGGATTTCGCGCTGCTGGGCACCCACGGAGCCTCCGGGGCTGCCGGCTTCGT
  CCAGGCGCCGCTGTCCCAGCAGAGGTGGGTGGGGGGCAGTGTGGAGCTGCACTGCGAGGC
  CGTGGGCAGCCCGGTGCCCGAGATCCAGTGGTGGTTTGAAGGGCAGGGTCCCAACGACAC
  CTGCTCCCAGCTCTGGGACGGCGCCCGGCTGGACCGCGTCCACATCCACGCCACCTACCA
  CCAGCACGCGGCCAGCACCATCTCCATCGACACGCTCGTGGAGGAGGACACGGGCACTTA
  CGAGTGCCGGGCCAGCAACGACCCGGATCGCAACCACCTGACCCGGGCGCCCAGGGTCAA
  GTGGGTCCGCGCCCAGGCAGTCGTGCTAGTCCTGGAACCCGGCACAGTCTTCACTACCGT
  AGAAGACCTTGGCTCCAAGATACTCCTCACCTGCTCCTTGAATGACAGCGCCACAGAGGT
  CACAGGGCACCGCTGGCTGAAGGGGGGCGTGGTGCTGAAGGAGGACGCGCTGCCCGGCCA
  GAAAACGGAGTTCAAGGTGGACTCCGACGACCAGTGGGGGAGAGTACTCCTGCGTCTTCC
  TCCCCGAGCCCATGGGGCACGGCCAACATCCAGCTCCACGGGCCTCCCAGAGTGAAAGCT
  GTGAAGTCGTCAGAACACATCAACGAGGGGAGACGGCCATGCTGGTCTGCAAGTCAGAGT
  CCGTGCCACCTGTCACTGACTGGGCCTGGTACAAGATCACTGACTCTGAGGACAGCCCTC
  ATGAACGCTCCGAGAGCAGTTCTTCGTGAGTTCCTCGCAGGCCCGTCAGAGCTACAACAT
  TGAGAACCTGAACATGGAGGCCGGACCCCGGCCAGT
  
  3' Read Nucleotide Sequence

  >Forward primer walk for NM_001728 unedited
  TAGCAACCGGCCTCCGAGCAGGTTCTTCGTGAGTTCCTCGCAGGGCCGGTCAGAGCTACA
  CATTGAGAACCTGAACATGGAGGCCGACCCCGGCCAGTACCGGTGCAACGGCACCAGCTC
  CAAGGGCTCCGACCAGGCCATCATCACGCTCCGCGTGCGCAGCCACCTGGCCGCCCTCTG
  GCCCTTCCTGGGCATCGTGGCTGAGGTGCTGGTGCTGGTCACCATCATCTTCATCTACGA
  GAAGCGCCGGAAGCCCGAGGACGTCCTGGATGATGACGACGCCGGCTCTGCACCCCTGAA
  GAGCAGCGGGCAGCACCAGAATGACAAAGGCAAGAACGTCCGCCAGAGGAACTCTTCCTG
  AGGCAGGTGGCCCGAGGACGCTCCCTGCTCCACGTCTGCGCCGCCGCCGGAGTCCACTCC
  CAGTGCTTGCAAGATTCCAAGTTCTCACCTCTTAAAGAAAACCCACCCCGTAGATTCCCA
  TCATACACTTCCTTCTTTTTTAAAAAAGTTGGGTTTTCTCCATTCAGGATTCTGTTCCTT
  AGGTTTTTTTCCTTCTGAAGTGTTTCACGAGAGCCCGGGAGCTGCTGCCCTGCGGCCCCG
  TCTGTGGCTTTCAGCCTCTGGGTCTGAGTCATGGCCGGGTGGGCGGCACAGCCTTCTCCA
  CTGGCCGGAGTCAGTGCCAGGTCCTTGCCCTTTGTGGAAAGTCACAGGTCACACGAGGGG
  CCCCGTGTCCTGCCTGTCTGAAGCCAATGCTGTCTGGTTGCGCCATTTTTGTGCTTTTAT
  GTTTAATTTTATGAGGGCCACGGGTCTGTGTTCGACTCAGCCTCAGGGACGACTCTGACC
  TCTTGGCCACAGAGGACTCACTTGCCCACACCGAGGGCGACCCCGTCACAGCCTCAAGTC
  ACTCCCAGCCCCCTCCTTGTCTGTGCATCGGGGCAGCTCTGGAGGGGGTTTGCTGGGGAC
  TGGCGCCATCGCCGGGACCTCCAGACCGC
  
• Restriction Sites: Please inquire
• ACCN: NM_001728
• Insert Size: 2000 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001728.2, NP_001719.2
• RefSeq Size: 2024 bp
• RefSeq ORF: 1158 bp
• Locus ID: 682
• UniProt ID: P35613
• Cytogenetics: 19p13.3
• Domains: ig, IG
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Transmembrane
• Gene Summary: The protein encoded by this gene, basigin, is a plasma membrane protein that is important in spermatogenesis, embryo implantation, neural network formation, and tumor progression. Basigin is also a member of the immunoglobulin superfamily, ubiquitously expressed in various tissues. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2020]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).