IKB epsilon (NFKBIE) (NM_004556) Human Untagged Clone

CAT#: SC122682

NFKBIE (untagged)-Human nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, epsilon (NFKBIE)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: IKB epsilon
• Synonyms: IKBE
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_004556 edited
  GGCACGAGGCCTGGCCAGCGCTCCGAACCTTGTCCTCGCTGCGCGCCGGCCCCTCGGAGC
  CCCACAGCCCGGGAAGGAGGCCGCCGCGGGCCGGGCGCCCGCTCTGCCAAGCGGACCCGC
  AACCCGGAAAGGCGGCGCGGCGGAGCCTGGAGCCGGATCCTGCTCAGACCGGGCCCCGGC
  CGGCCAGAGCCGCGGGCATGTCGGAGGCGCGGAAGGGGCCGGACGAGGCGGAGGAGAGCC
  AGTACGACTCTGGCATTGAGTCTCTGCGCTCTCTGCGCTCCCTACCCGAGTCCACCTCGG
  CTCCAGCCTCCGGGCCCTCGGACGGCAGCCCCCAGCCCTGCACCCATCCTCCGGGACCCG
  TCAAGGAACCACAGGAGAAGGAAGACGCGGATGGGGAGCGGGCTGATTCCACCTATGGCT
  CCTCCTCGCTCACCTACACCCTGTCCTTGCTGGGGGGCCCCGAGGCTGAGGACCCGGCCC
  CACGCCTGCCACTCCCCCACGTGGGGGCGCTGAGCCCTCAGCAGCTGGAAGCACTCACTT
  ACATCTCCGAGGACGGAGACACGCTGGTCCACCTGGCAGTGATTCATGAGGCCCCAGCGG
  TGCTGCTCTGTTGCCTGGCTTTGCTGCCCCAGGAGGTCCTGGACATTCAAAATAACCTTT
  ACCAGACAGCACTCCATCTGGCTGTACATCTGGACCAACCGGGCGCAGTTCGGGCACTGG
  TGCTGAAGGGGGCCAGCCGGGCACTACAGGACCGGCATGGTGACACAGCCCTTCATGTGG
  CCTGCCAGCGCCAGCACTTGGCCTGTGCCCGCTGCCTGCTGGAAGGGCGGCCAGAGCCAG
  GCAGAGGAACATCTCACTCTCTGGACCTCCAGCTGCAAAACTGGCAAGGTCTGGCTTGTC
  TCCACATTGCCACCCTTCAGAAGAACCAACCACTCATGGAATTGCTGCTTCGGAATGGAG
  CTGACATTGATGTGCAGGAGGGCACCAGTGGTAAGACAGCGCTGCACCTGGCTGTGGAAA
  CCCAAGAGCGGGGCCTGGTACAGTTCCTGCTCCAGGCTGGTGCCCAGGTAGATGCCCGCA
  TGCTGAACGGGTGCACACCCCTGCACCTGGCAGCTGGCCGGGGTCTCATGGGCATCTCAT
  CCACTCTGTGCAAGGCGGGTGCTGACTCCCTGCTGCGGAATGTGGAGGATGAGACGCCCC
  AGGACCTGACTGAGGAATCCCTTGTCCTTTTGCCCTTTGATGACCTGAAGATCTCAGGGA
  AACTGCTGCTGTGTACCGACTGAAGCCAGGCAGGGTCTGGGATCCTCAGGGCTCCACCTC
  TCCATCTGGAAGCCGGAGCCATAACTGCTGCAGTTTGGGCCCAGGCTATGTGCTCTTCTG
  GTGCCCTAGGGACTGCTGTGGCCAGAGCCTGGGGCCAGCCAGTACAGTCCTGAGCCGAGG
  AGGAGGGACTGCAAGTGGAAGAGAGCCAGTCTGGAAGGAAGAGCTTTCCAGGTGGACAGG
  GCTTCTTGGAAGACCCCCAAAGCCCCAGGTATCCTGGGTGAAGCCTGTTTGCCTCTCTTG
  AAAATGGCAGGTGCTCTTGTTTTACCCATGTTGGGTCAGCCTGAAACTGCCAACCAGTAG
  GAAGCATGGACTCTCCTGAGTGAGAAGAGACTGAAATAGGAGCAAGCAGAACCCTGAGAG
  GTGTCCCATCTTATTGCTGTTGAGGACCCTGAAACACCGTTGTTTAAAGACTTCACACAG
  AAGGCTCTGAACTGGGCCACTGGGGAAGGGAAGTTTCAGTAACATGACACTAAAATGGCA
  GAGACGTTAAAAAAAGTTTTTCCCTTCTAAAAAAAAAAAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004556 unedited
  GGCCAACTTCAGTAANNTGTAAACACGACNCACTATNNAGGGCGGCCGCGCAATTCGGCA
  CGAGGCCTGGCCAGCGCTCCGAACCTTGTCCTCGCTGCGCGCCGGCCCCTCGGAGCCCCA
  CAGCCCGGGAAGGAGGCCGCCGCGGGCCGGGCGCCCGCTCTGCCAAGCGGACCCGCAACC
  CGGAAAGGCGGCGCGGCGGAGCCTGGAGCCGGATCCTGCTCAGACCGGGCCCCGGCCGGC
  CAGAGCCGCGGGCATGTCGGAGGCGCGGAAGGGGCCGGACGAGGCGGAGGAGAGCCAGTA
  CGACTCTGGCATTGAGTCTCTGCGCTCTCTGCGCTCCCTACCCGAGTCCACCTCGGCTCC
  AGCCTCCGGGCCCTCGGACGGCAGCCCCCAGCCCTGCACCCATCCTCCGGGACCCGTCAA
  GGAACCACAGGAGAAGGAAGACGCGGATGGGGAGCGGGCTGATTCCACCTATGGCTCCTC
  CTCGCTCACCTACACCCTGTCCTTGCTGGGGGGCCCCGAGGCTGAGGACCCGGCCCCACG
  CCTGCCACTCCCCCACGTGGGGGCGCTGAGCCCTCAGCAGCTGGAAGCACTCACTTACAT
  CTCCGAGGACGGAGACACGCTGGTCCACCTGGCAGTGATTCATGAGGCCCCAGCGGTGCT
  GCTCTGTTGCCTGGCTTTGCTGCCCCAGGAGGTCCTGGACATTCAAAATAACCTTTACCA
  GACAGCACTCCATCTGGCTGTACATCTGGACCAACCGGGCGCAGTTCGGGCACTGGTGCT
  NGAAGGGGCCAGCCGGGCACTACAGGACCGGCATGGTGACACAGCCCTTCATGTGGCCTG
  CCAGCGCCAGCACTTGGCCTGTGCCCGCTGNCTGCTGGAAGGGCNGNNCAGAGCCAGCAG
  AGGAACATCTCACTCTT
  
• Restriction Sites: Please inquire
• ACCN: NM_004556
• Insert Size: 1847 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004556.2, NP_004547.2
• RefSeq Size: 2581 bp
• RefSeq ORF: 1503 bp
• Locus ID: 4794
• UniProt ID: O00221
• Cytogenetics: 6p21.1
• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Adipocytokine signaling pathway, B cell receptor signaling pathway, Neurotrophin signaling pathway, T cell receptor signaling pathway
• Gene Summary: The protein encoded by this gene binds to components of NF-kappa-B, trapping the complex in the cytoplasm and preventing it from activating genes in the nucleus. Phosphorylation of the encoded protein targets it for destruction by the ubiquitin pathway, which activates NF-kappa-B by making it available to translocate to the nucleus. [provided by RefSeq, Sep 2011]