CD62E (SELE) (NM_000450) Human Untagged Clone

CAT#: SC119869

SELE (untagged)-Human selectin E (SELE)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CD62E
• Synonyms: CD62E; ELAM; ELAM1; ESEL; LECAM2
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119869 sequence for NM_000450 edited (data generated by NextGen Sequencing)
  ATGATTGCTTCACAGTTTCTCTCAGCTCTCACTTTGGTGCTTCTCATTAAAGAGAGTGGA
  GCCTGGTCTTACAACACCTCCACGGAAGCTATGACTTATGATGAGGCCAGTGCTTATTGT
  CAGCAAAGGTACACACACCTGGTTGCAATTCAAAACAAAGAAGAGATTGAGTACCTAAAC
  TCCATATTGAGCTATTCACCAAGTTATTACTGGATTGGAATCAGAAAAGTCAACAATGTG
  TGGGTCTGGGTAGGAACCCAGAAACCTCTGACAGAAGAAGCCAAGAACTGGGCTCCAGGT
  GAACCCAACAATAGGCAAAAAGATGAGGACTGCGTGGAGATCTACATCAAGAGAGAAAAA
  GATGTGGGCATGTGGAATGATGAGAGGTGCAGCAAGAAGAAGCTTGCCCTATGCTACACA
  GCTGCCTGTACCAATACATCCTGCAGTGGCCACGGTGAATGTGTAGAGACCATCAATAAT
  TACACTTGCAAGTGTGACCCTGGCTTCAGTGGACTCAAGTGTGAGCAAATTGTGAACTGT
  ACAGCCCTGGAATCCCCTGAGCATGGAAGCCTGGTTTGCAGTCACCCACTGGGAAACTTC
  AGCTACAATTCTTCCTGCTCTATCAGCTGTGATAGGGGTTACCTGCCAAGCAGCATGGAG
  ACCATGCAGTGTATGTCCTCTGGAGAATGGAGTGCTCCTATTCCAGCCTGCAATGTGGTT
  GAGTGTGATGCTGTGACAAATCCAGCCAATGGGTTCGTGGAATGTTTCCAAAACCCTGGA
  AGCTTCCCATGGAACACAACCTGTACATTTGACTGTGAAGAAGGATTTGAACTAATGGGA
  GCCCAGAGCCTTCAGTGTACCTCATCTGGGAATTGGGACAACGAGAAGCCAACGTGTAAA
  GCTGTGACATGCAGGGCCGTCCGCCAGCCTCAGAATGGCTCTGTGAGGTGCAGCCATTCC
  CCTGCTGGAGAGTTCACCTTCAAATCATCCTGCAACTTCACCTGTGAGGAAGGCTTCATG
  TTGCAGGGACCAGCCCAGGTTGAATGCACCACTCAAGGGCAGTGGACACAGCAAATCCCA
  GTTTGTGAAGCTTTCCAGTGCACAGCCTTGTCCAACCCCGAGCGAGGCTACATGAATTGT
  CTTCCTAGTGCTTCTGGCAGTTTCCGTTATGGGTCCAGCTGTGAGTTCTCCTGTGAGCAG
  GGTTTTGTGTTGAAGGGATCCAAAAGGCTCCAATGTGGCCCCACAGGGGAGTGGGACAAC
  GAGAAGCCCACATGTGAAGCTGTGAGATGCGATGCTGTCCACCAGCCCCCGAAGGGTTTG
  GTGAGGTGTGCTCATTCCCCTATTGGAGAATTCACCTACAAGTCCTCTTGTGCCTTCAGC
  TGTGAGGAGGGATTTGAATTACATGGATCAACTCAACTTGAGTGCACATCTCAGGGACAA
  TGGACAGAAGAGGTTCCTTCCTGCCAAGTGGTAAAATGTTCAAGCCTGGCAGTTCCGGGA
  AAGATCAACATGAGCTGCAGTGGGGAGCCCGTGTTTGGCACTGTGTGCAAGTTCGCCTGT
  CCTGAAGGATGGACGCTCAATGGCTCTGCAGCTCGGACATGTGGAGCCACAGGACACTGG
  TCTGGCCTGCTACCTACCTGTGAAGCTCCCACTGAGTCCAACATTCCCTTGGTAGCTGGA
  CTTTCTGCTGCTGGACTCTCCCTCCTGACATTAGCACCATTTCTCCTCTGGCTTCGGAAA
  TGCTTACGGAAAGCAAAGAAATTTGTTCCTGCCAGCAGCTGCCAAAGCCTTGAATCAGAT
  GGAAGCTACCAAAAGCCTTCTTACATCCTTTAA
  
  Clone variation with respect to NM_000450.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000450 unedited
  AAAATATTTGTATACGACTCACTATAGGGCGGCNCGCGAATTCGCACGAAAAAAACTCCT
  ATACTGACCTGAGACAGAGGCAGCAGTGATACCCACCTGAGAGATCCTGTGTTTGAACAA
  CTGCTTCCCAAAACGGAAAGTATTTCAAGCCTAAACCTTTGGGTGAAAAGAACTCTTGAA
  GTCATGATTGCTTCACAGTTTCTCTCAGCTCTCACTTTGGTGCTTCTCATTAAAGAGAGT
  GGAGCCTGGTCTTACAACACCTCCACGGAAGCTATGACTTATGATGAGGCCAGTGCTTAT
  TGTCAGCAAAGGTACACACACCTGGTTGCAATTCAAAACAAAGAAGAGATTGAGTACCTA
  AACTCCATATTGAGCTATTCACCAAGTTATTACTGGATTGGAATCAGAAAAGTCAACAAT
  GTGTGGGTCTGGGTAGGAACCCAGAAACCTCTGACAGAAGAAGCCAAGAACTGGGCTCCA
  GGTGAACCCAACAATAGGCAAAAAGATGAGGACTGCGTGGAGATCTACATCAAGAGAGAA
  AAAGATGTGGGCATGTGGAATGATGAGAGGTGCAGCAAGAAGAAGCTTGCCCTATGCTAC
  ACAGCTGCCTGTACCAATACATCCTGCAGTGGCCCACGGTGAATGTGTAGAGACCATCAA
  TAATTACACTTGCAAGTGTGACCCTGGCTTCAGTGGACTCAAGTGTGAGCAAATTGTGAA
  CTGTACAGCCCTGGAATCCCCTGAGCATGGAACCCTGTTTTGCAGTCACCCACTGGGAAA
  CTTCAGCTACCATTCTTCCTGCTCTATCAGCTGTGAAAAAGGGGGTTACCTGCCAAGCAG
  CATGGAGACCATGCNANNGNATGTCCTCTGGAGAAAAAGGGAGTGCTCCTATTCCACCTG
  CCATGGTGGGGNGGAGTGGGAATGCTGGGACAAATCCGNCCACGGGGGTTCGGGGAAAAA
  GTTACA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000450 unedited
  CTTGGACGCGGCCGCATTCTAGGATCGAGTTTTTTTTTTTTTTTTTTTTAAACATAAGCA
  CATTTATTGTCAACCTTTATAGTGTTATGTCAAATAGGTCTGACATAAGCTTAAATAAAT
  ATATACTTTAAAAATTATAAAATATTTTAAGTTATAATTTAAAATTCTCAATAAAACTCA
  AACACAAACCACACTGGTATTTCACACAGCTAATTTCTAATGCAGTTTACATAAATATTT
  ACAACACTTAAACAATTTCAAAGAAAATAACACTGTATTCCATACATAGCCTGATCACAG
  TAGTTGTTCTCTCTTATTTCCCAGAGTTTTTCTGCCCCTTTAAAAGAACCTCTGCTGTTC
  TGATCCTTATCACATCTCTGTTTTGACTGTTGGCTTTGTTGTTGCCAGTGTTCAGCCAGA
  ACTTCTCTGAAACTTTTTTTTCAACACATGCTAAGTTAATGGAAGTGTAGGAGAGTTTTG
  ATTCTCACACTCCTCAAGGCTAGAGCAGCTTTGGCAATTACTGACTGAGAATTTTTCATT
  GCCAGTGATCAACTGAAAACTGGAGATTCCTTTGGAATTGTTAAATCTGCTTATAAATAA
  ACATAAATGCTTGCTCACACAGGCATTCCTCTCTTCCAGAGCACCCTAACATACAGAAGA
  AAACAAATAGGGAATAACTATTAGACATCTTCATTCGNTAAAAAATCTACCAGATGACTC
  TTTTACATGGTGAGTTTCTATTGTGAAATTAAAATCTTCCATAATATACAAGAANTATGT
  TTACATATCATATCTGACAACATCTTTGTAGAATGCCAACACATCCATCTTCTGTATTCT
  TTCCACAAAAACATTATAAATTATACTTTGNGTGGTTGCATTATGCTTTATTAGTCAAAA
  CGTTGGCCTATGGAGTTTCATCGTGAAACACTATTCTGAAAAATCTGACATAACAAACTT
  CATCATT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000450
• Insert Size: 4000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000450.1, NP_000441.1
• RefSeq Size: 3834 bp
• RefSeq ORF: 1833 bp
• Locus ID: 6401
• UniProt ID: P16581
• Cytogenetics: 1q24.2
• Domains: CCP, CLECT, EGF
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Cell adhesion molecules (CAMs)
• Gene Summary: The protein encoded by this gene is found in cytokine-stimulated endothelial cells and is thought to be responsible for the accumulation of blood leukocytes at sites of inflammation by mediating the adhesion of cells to the vascular lining. It exhibits structural features such as the presence of lectin- and EGF-like domains followed by short consensus repeat (SCR) domains that contain 6 conserved cysteine residues. These proteins are part of the selectin family of cell adhesion molecules. Adhesion molecules participate in the interaction between leukocytes and the endothelium and appear to be involved in the pathogenesis of atherosclerosis. [provided by RefSeq, Jul 2008]