TGF beta 1 (TGFB1) (NM_000660) Human Untagged Clone

CAT#: SC119746

TGFB1 (untagged)-Human transforming growth factor, beta 1 (TGFB1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TGF beta 1
• Synonyms: CED; DPD1; IBDIMDE; LAP; TGF-beta1; TGFB; TGFbeta
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_000660 edited
  GAATTCGGCACCAGGCGTCCCTCAGGCGCCCCCATTCCGGACCAGCCCTCGGGAGTCGCC
  GACCCGGCCTCCCGCAAAGACTTTTCCCCAGACCTCGGGCGCACCCCCTGCACGCCGCCT
  TCATCCCCGGCCTGTCTCCTGAGCCCCCGCGCATCCTAGACCCTTTCTCCTCCAGGAGAC
  GGATCTCTCTCCGACCTGCCACAGATCCCCTATTCAAGACCACCCACCTTCTGGTACCAG
  ATCGCGCCCATCTAGGTTATTTCCGTGGGATACTGAGACACCCCCGGTCCAAGCCTCCCC
  TCCACCACTGCGCCCTTCTCCCTGAGGACCTCAGCTTTCCCTCGAGGCCCTCCTACCTTT
  TGCCGGGAGACCCCCAGCCCCTGCAGGGGCGGGGCCTCCCCACCACACCAGCCCTGTTCG
  CGCTCTCGGCAGTGCCGGGGGGCGCCGCCTCCCCCATGCCGCCCTCCGGGCTGCGGCTGC
  TGCCGCTGCTGCTACCGCTGCTGTGGCTACTGGTGCTGACGCCTGGCCGGCCGGCCGCGG
  GACTATCCACCTGCAAGACTATCGACATGGAGCTGGTGAAGCGGAAGCGCATCGAGGCCA
  TCCGCGGCCAGATCCTGTCCAAGCTGCGGCTCGCCAGCCCCCCGAGCCAGGGGGAGGTGC
  CGCCCGGCCCGCTGCCCGAGGCCGTGCTCGCCCTGTACAACAGCACCCGCGACCGGGTGG
  CCGGGGAGAGTGCAGAACCGGAGCCCGAGCCTGAGGCCGACTACTACGCCAAGGAGGTCA
  CCCGCGTGCTAATGGTGGAAACCCACAACGAAATCTATGACAAGTTCAAGCAGAGTACAC
  ACAGCATATATATGTTCTTCAACACATCAGAGCTCCGAGAAGCGGTACCTGAACCCGTGT
  TGCTCTCCCGGGCAGAGCTGCGTCTGCTGAGGCTCAAGTTAAAAGTGGAGCAGCACGTGG
  AGCTGTACCAGAAATACAGCAACAATTCCTGGCGATACCTCAGCAACCGGCTGCTGGCAC
  CCAGCGACTCGCCAGAGTGGTTATCTTTTGATGTCACCGGAGTTGTGCGGCAGTGGTTGA
  GCCGTGGAGGGGAAATTGAGGGCTTTCGCCTTAGCGCCCACTGCTCCTGTGACAGCAGGG
  ATAACACACTGCAAGTGGACATCAACGGGTTCACTACCGGCCGCCGAGGTGACCTGGCCA
  CCATTCATGGCATGAACCGGCCTTTCCTGCTTCTCATGGCCACCCCGCTGGAGAGGGCCC
  AGCATCTGCAAAGCTCCCGGCACCGCCGAGCCCTGGACACCAACTATTGCTTCAGCTCCA
  CGGAGAAGAACTGCTGCGTGCGGCAGCTGTACATTGACTTCCGCAAGGACCTCGGCTGGA
  AGTGGATCCACGAGCCCAAGGGCTACCATGCCAACTTCTGCCTCGGGCCCTGCCCCTACA
  TTTGGAGCCTGGACACGCAGTACAGCAAGGTCCTGGCCCTGTACAACCAGCATAACCCGG
  GCGCCTCGGCGGCGCCGTGCTGCGTGCCGCAGGCGCTGGAGCCGCTGCCCATCGTGTACT
  ACGTGGGCCGCAAGCCCAAGGTGGAGCAGCTGTCCAACATGATCGTGCGCTCCTGCAAGT
  GCAGCTGAGGTCCCGCCCCGCCCCGCCCCGCCCCGGCAGGCCCGGCCCCACCCCGCCCCG
  CCCCCGCTGCCTTGCCCATGGGGGCTGTATTTAAGGACACCCGTGCCCCAAGCCCACCTG
  GGGCCCCATTAAAGATGGAGAGAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
  AAAAAAAAACTCGAC
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000660 unedited
  TAAGATTTGTAATACGACTCACTATAGGGCGGCCGCGNAATTCGCACCAGGCGTCCCTCA
  GGCGCCCCCATTCCGGACCAGCCCTCGGGAGTCGCCGACCCGGCCTCCCGCAAAGACTTT
  TCCCCAGACCTCGGGCGCACCCCCTGCACGCCGCCTTCATCCCCGGCCTGTCTCCTGAGC
  CCCCGCGCATCCTAGACCCTTTCTCCTCCAGGAGACGGATCTCTCTCCGACCTGCCACAG
  ATCCCCTATTCAAGACCACCCACCTTCTGGTACCAGATCGCGCCCATCTAGGTTATTTCC
  GTGGGATACTGAGACACCCCCGGTCCAAGCCTCCCCTCCACCACTGCGCCCTTCTCCCTG
  AGGACCTCAGCTTTCCCTCGAGGCCCTCCTACCTTTTGCCGGGAGACCCCCAGCCCCTGC
  AGGGGCGGGGCCTCCCCACCACACCAGCCCTGTTCGCGCTCTCGGCAGTGCCGGGGGGCG
  CCGCCTCCCCCATGCCGCCCTCCGGGCTGCGGCTGCTGCCGCTGCTGCTACCGCTGCTGT
  GGCTACTGGTGCTGACGCCTGGCCGGCCGGCCGCGGGACTATCCACCTGCAAGACTATCG
  ACATGGAGCTGGTGAAGCGGAAGCGCATCGAGGCCATCCGCGGCCAGATCCTGTCCAAGC
  TGCGGCTCGCCAGCCCCCCGAGCCAGGGGGGAGGTGCCGCCCGGCCCGCTGCCCGAGGCC
  GTGCTCGCCCTGTACAACAGCACCCGCGACCGGGTGGCCGGNGAGAGTGCANAACCGGAG
  CCCGAGCCCTGAGCCGACTACTACGCCAAGGNAGTCACCCGCGTGCTAATGGGTGGAAAC
  CCACANNCGAATCTATGACAGTCTCAGCAGAGTCACACAGCATATTATATGTTCTTCACA
  CATCAGAGCTCCGAGAAGCGGACCTGAACCCCGTGTGCTCTCCCGGCAAAGCTGCGTCTG
  CTGAGGCTCAGT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000660 unedited
  AACCGCGAAACCCACACTTTTNNNNNNGTTTACTTGAACCCGGCCGCAATCTANATCGAG
  TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCTCTCTCCATCTTAA
  AGGGGGCCCCAGGGGGGCTTGGGGCACGGGTGTCCTTAAAACACCCCCCATGGGCAAGGC
  ACCGGGGGCGGGGCGGGGTGGGGCCGGCCCTGCCGGGGCGGGGCGGGGCGGGGCGGAACC
  TCATTTGCACTTGCAGGAGCCCACAATCATGTTGGACAGCTGCTCCACCTTGGGCTTGCG
  GCCCACGTATAACACAATGGGCAGGGGTTCCAGCGCCTGGGGAACCCAAAACGGCCCCCC
  CGAGGCGCCCGGGTTATGCTGGTTGTACAGGGCCAGAACCTTGCTGTACTGCGTGTCCAG
  CCTCCAAATGTAGGGGCAGGGCCCAAGGCAAAAGTTGGCATGGAACCCCTTGGGCTCGGG
  GATCCACTTCCAGCCGAGGTCCTTGCGGAAGTAAATGTACAGCTGCCGAACCCAACAGTT
  TTTTTCCGTGGAGCTAGAACAATATTTGGTGTCCAGGGCTCGGCGGTGCCGGAAGCTTTG
  CAAATGCTGGGCCCCTCTCCAGCGGGGTGGCCATGAAAAACAAGAAAAGGCCGGTTATTG
  CCATGAATGTTGGCCAGGTCACCTCGGCGGCCGGTAGTAAACCCTTTGATGTCCACTTGC
  AGGGTGTATCCCTGCTGCAAAGAAACAGGGGCCCTTAAGGCAAAGCCCTCAATTTCCCCT
  CCAGGGTAACCCATTGCGAAAATTTCGGGGAAATAAAAAAAACCCCTTTGGCAATCCTTG
  GGGTCCACAAACCGGTTGTGAGGTATCCACAGAATTTTTTTTTTTTTTGGACAGCTCCCG
  GCGGTCCCCTTTAACTGGAACCCAAAAAACCCACTTTTGCCGAAAAAACACGGTTTT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000660
• Insert Size: 1870 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: TRueClone
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000660.3, NP_000651.3
• RefSeq Size: 2346 bp
• RefSeq ORF: 1173 bp
• Locus ID: 7040
• UniProt ID: P01137
• Cytogenetics: 19q13.2
• Domains: TGFb_propeptide, TGF-beta
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Secreted Protein, Transcription Factors
• Protein Pathways: Cell cycle, Chronic myeloid leukemia, Colorectal cancer, Cytokine-cytokine receptor interaction, Dilated cardiomyopathy, Hypertrophic cardiomyopathy (HCM), MAPK signaling pathway, Pancreatic cancer, Pathways in cancer, Renal cell carcinoma, TGF-beta signaling pathway
• Gene Summary: This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate a latency-associated peptide (LAP) and a mature peptide, and is found in either a latent form composed of a mature peptide homodimer, a LAP homodimer, and a latent TGF-beta binding protein, or in an active form consisting solely of the mature peptide homodimer. The mature peptide may also form heterodimers with other TGFB family members. This encoded protein regulates cell proliferation, differentiation and growth, and can modulate expression and activation of other growth factors including interferon gamma and tumor necrosis factor alpha. This gene is frequently upregulated in tumor cells, and mutations in this gene result in Camurati-Engelmann disease. [provided by RefSeq, Aug 2016]