Factor D (CFD) (NM_001928) Human Untagged Clone

CAT#: SC118950

CFD (untagged)-Human complement factor D (adipsin) (CFD)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Factor D
• Synonyms: ADIPSIN; ADN; DF; PFD
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118950 sequence for NM_001928 edited (data generated by NextGen Sequencing)
  ATGCACAGCTGGGAGCGCCTGGCAGTTCTGGTCCTCCTAGGAGCGGCCGCCTGCGCGGCG
  CCGCCCCGTGGTCGGATCCTGGGCGGCAGAGAGGCCGAGGCGCACGCGCGGCCCTACATG
  GCGTCGGTGCAGCTGAACGGCGCGCACCTGTGCGGCGGCGTCCTGGTGGCGGAGCAGTGG
  GTGCTGAGCGCGGCGCACTGCCTGGAGGACGCGGCCGACGGGAAGGTGCAGGTTCTCCTG
  GGCGCGCACTCCCTGTCGCAGCCGGAGCCCTCCAAGCGCCTGTACGACGTGCTCCGCGCA
  GTGCCCCACCCGGACAGCCAGCCCGACACCATCGACCACGACCTCCTGCTGCTACAGCTG
  TCGGAGAAGGCCACACTGGGCCCTGCTGTGCGCCCCCTGCCCTGGCAGCGCGTGGACCGC
  GACGTGGCACCGGGAACTCTCTGCGACGTGGCCGGCTGGGGCATAGTCAACCACGCGGGC
  CGCCGCCCGGACAGCCTGCAGCACGTGCTCTTGCCAGTGCTGGACCGCGCCACCTGCAAC
  CGGCGCACGCACCACGACGGCGCCATCACCGAGCGCTTGATGTGCGCGGAGAGCAATCGC
  CGGGACAGCTGCAAGGGTGACTCCGGGGGCCCGCTGGTGTGCGGGGGCGTGCTCGAGGGC
  GTGGTCACCTCGGGCTCGCGCGTTTGCGGCAACCGCAAGAAGCCCGGGATCTACACCCGC
  GTGGCGAGCTATGCGGCCTGGATCGACAGCGTCCTGGCCTAG
  
  Clone variation with respect to NM_001928.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001928 unedited
  NTTGTTCGAATATTTGTATACGACTCACTTATAGGGCGGCCGCGATTCGGCACCAGCTTC
  ACCATGCACAGCTGGGAGCGCCTGGCAGTTCTGGTCCTCCTAGGAGCGGCCGCCTGCGCG
  GCGCCGCCCCGTGGTCGGATCCTGGGCGGCAGAGAGGCCGAGGCGCACGCGCGGCCCTAC
  ATGGCGTCGGTGCAGCTGAACGGCGCGCACCTGTGCGGCGGCGTCCTGGTGGCGGAGCAG
  TGGGTGCTGAGCGCGGCGCACTGCCTGGAGGACGCGGCCGACGGGAAGGTGCAGGTTCTC
  CTGGGCGCGCACTCCCTGTCGCAGCCGGAGCCCTCCAAGCGCCTGTACGACGTGCTCCGC
  GCAGTGCCCCACCCGGACAGCCAGCCCGACACCATCGACCACGACCTCCTGCTGCTACAG
  CTGTCGGAGAAGGCCACACTGGGCCCTGCTGTGCGCCCCCTGCCCTGGCAGCGCGTGGAC
  CGCGACGTGGCACCGGGAACTCTCTGCGACGTGGCCGGCTGGGGCATAGTCAACCACGCG
  GGCCGCCGCCCGGACAGCCTGCAGCACGTGCTCTTGCCAGTGCTGGACCGCGCCACCTGC
  AACCGGCGCACGCACCACGACGGCGCCATCACCGAGCGCTTGATGTGCGCGGAGAGCAAT
  CGCCGTGACAGCTGCAAGGGTGACTCCGGNGGCCCGCTGGTGTGCGGNGGCGTGCTCGAG
  GGCGTGGTCCCCTCGGCTCGCGCGTTTGCGGCAACCGCAAGAAGCCCGGGATCTACACCC
  GCGTGGCGAGCTATGCGGCCTGGATCGACAGCGTTCTGGCCTAGGGTGCCGGGGCCTGAA
  GGTCAGGGTCACCCAAGCAACAAAGTCCCGAGCAATGAAGTCATCCACTCCTGCATCTGG
  TTGGGCTTTTATGAGCACCTACTTATGCAGAGGNGGAGG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001928 unedited
  NACCCCAAAAAAACAAACACAAACCACCCCCAAAACCCTTACTTGAACGGGCCGCTTTCT
  ANATCGAGANTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCCCAGCTAATTTTTTAT
  TTATTTGTAAAGATGGAGTCGCGCTACGTGGCCCATGCTGATCTCGAACTCCTGAGATCC
  AATGATCCTCCCACCTTGGGCTCCCCTTCTGCATATAGTAGGGGCTTAATAAAGACCAAC
  CAGATGCCGGAGCGGACGACTTCATTGCTCGGGACTTTGTTGCTTGGGTGACCCTGACCT
  TCAAGCCCCGGCACCCTAGGCCAGGACCCTGTCGATCCAGGCCGGATAGTTTGTCCCGCG
  GGTGTACATCCCCGGCTTCTTGGGGTTGGCGTACACGCGCGAGCCCGAGGTGACCACACC
  CTCAATCACGCCCCCGCAAACCCCCCGGCCCCCCTGGCATCACCCTTCCACCCTGTCCGC
  CCACTGGTTTTCGCGCACATCACAGCCTCCTCGATGTGCCCCCCCTTTCGTGCCCACCCC
  CTCACGCCCGAGGCGCCCCACCCCCACTCGCCATTAACCCTATCCCAACCCATCCCCGTG
  TGTCACCCCACGCCGCCCACAATTCCTATTCCCCCCATTACCCTAGACTTATTCCCCCAA
  GCCCACCTCATCTCCCCACCTAACCCTTGCCCCCTCCCCTCCCTCTTCCCCCCCTCCCCC
  CTTTCCCCCTCTCACCCCTTTTGCCCCGGCCTCCCTTCCCTTCCTTCCCATTTTCCCTTT
  TGTCTCTTCTCTACTCCTCTCACTGCCTCCTCTGCCCTCTCGTTCCCCCTCTTCTTCTCC
  TCCCCCCCCTCCTCCTTCCCTTCCCTCATCCCATTGCTTCCCCCCTTATTCTCCTATCCC
  TTTCTCACCCTCCCCTCCCCCCTTTTTTN
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001928
• Insert Size: 1020 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001928.2, NP_001919.2
• RefSeq Size: 1173 bp
• RefSeq ORF: 762 bp
• Locus ID: 1675
• UniProt ID: P00746
• Cytogenetics: 19p13.3
• Domains: Tryp_SPc
• Protein Families: Druggable Genome, Protease, Secreted Protein
• Protein Pathways: Complement and coagulation cascades
• Gene Summary: This gene encodes a member of the S1, or chymotrypsin, family of serine peptidases. This protease catalyzes the cleavage of factor B, the rate-limiting step of the alternative pathway of complement activation. This protein also functions as an adipokine, a cell signaling protein secreted by adipocytes, which regulates insulin secretion in mice. Mutations in this gene underlie complement factor D deficiency, which is associated with recurrent bacterial meningitis infections in human patients. Alternative splicing of this gene results in multiple transcript variants. At least one of these variants encodes a preproprotein that is proteolytically processed to generate the mature protease. [provided by RefSeq, Nov 2015]
  Transcript Variant: This variant (1) represents the shorter transcript and encodes the shorter isoform (1).