GUCY2C (NM_004963) Human Untagged Clone

CAT#: SC117052

GUCY2C (untagged)-Human guanylate cyclase 2C (heat stable enterotoxin receptor) (GUCY2C)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GUCY2C
• Synonyms: DIAR6; GC-C; GUC2C; MECIL; MUCIL; STAR
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_004963 edited
  GCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAACCGTCAGA
  ATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACCAGCTGGATTCCCTGC
  CTCCCTAGGAGCCCAACAGAGCAAAGCAAGTGGGCACAAGGAGTATGGTTCTAACGTGAT
  TGGGGTCATGAAGACGTTGCTGTTGGACTTGGCTTTGTGGTCACTGCTCTTCCAGCCCGG
  GTGGCTGTCCTTTAGTTCCCAGGTGAGTCAGAACTGCCACAATGGCAGCTATGAAATCAG
  CGTCCTGATGATGGGCAACTCAGCCTTTGCAGAGCCCCTGAAAAACTTGGAAGATGCGGT
  GAATGAGGGGCTGGAAATAGTGAGAGGACGTCTGCAAAATGCTGGCCTAAATGTGACTGT
  GAACGCTACTTTCATGTATTCGGATGGTCTGATTCATAACTCAGGCGACTGCCGGAGTAG
  CACCTGTGAAGGCCTCGACCTACTCAGGAAAATTTCAAATGCACAACGGATGGGCTGTGT
  CCTCATAGGGCCCTCATGTACATACTCCACCTTCCAGATGTACCTTGACACAGAATTGAG
  CTACCCCATGATCTCAGCTGGAAGTTTTGGATTGTCATGTGACTATAAAGAAACCTTAAC
  CAGGCTGATGTCTCCAGCTAGAAAGTTGATGTACTTCTTGGTTAACTTTTGGAAAACCAA
  CGATCTGCCCTTCAAAACTTATTCCTGGAGCACTTCGTATGTTTACAAGAATGGTACAGA
  AACTGAGGACTGTTTCTGGTACCTTAATGCTCTGGAGGCTAGCGTTTCCTATTTCTCCCA
  CGAACTCGGCTTTAAGGTGGTGTTAAGACAAGATAAGGAGTTTCAGGATATCTTAATGGA
  CCACAACAGGAAAAGCAATGTGATTATTATGTGTGGTGGTCCAGAGTTCCTCTACAAGCT
  GAAGGGTGACCGAGCAGTGGCTGAAGACATTGTCATTATTCTAGTGGATCTTTTCAATGA
  CCAGTACTTGGAGGACAATGTCACAGCCCCTGACTATATGAAAAATGTCCTTGTTCTGAC
  GCTGTCTCCTGGGAATTCCCTTCTAAATAGCTCTTTCTCCAGGAATCTATCACCAACAAA
  ACGAGACTTTGCTCTTGCCTATTTGAATGGAATCCTGCTCTTTGGACATATGCTGAAGAT
  ATTTCTTGAAAATGGAGAAAATATTACCACCCCCAAATTTGCTCATGCTTTCAGGAATCT
  CACTTTTGAAGGGTATGACGGTCCAGTGACCTTGGATGACTGGGGGGATGTTGACAGTAC
  CATGGTGCTTCTGTATACCTCTGTGGACACCAAGAAATACAAGGTTCTTTTGACCTATGA
  TACCCACGTAAATAAGACCTATCCTGTGGATATGAGCCCCACATTCACTTGGAAGAACTC
  TAAACTTCCTAATGATATTACAGGCCGGGGCCCTCAGATCCTGATGATTGCAGTCTTCAC
  CCTCACTGGAGCTGTGGTGCTGCTCCTGCTCGTCGCTCTCCTGATGCTCAGAAAATATAG
  AAAAGATTATGAACTTCGTCAGAAAAAATGGTCCCACATTCCTCCTGAAAATATCTTTCC
  TCTGGAGACCAATGAGACCAATCATGTTAGCCTCAAGATCGATGATGACAAAAGACGAGA
  TACAATCCAGAGACTACGACAGTGCAAATACGACAAAAAGCGAGTGATTCTCAAAGATCT
  CAAGCACAATGATGGTAATTTCACTGAAAAACAGAAGATAGAATTGAACAAGTTGCTTCA
  GATTGACTATTACAACCTGACCAAGTTCTACGGCACAGTGAAACTTGATACCATGATCTT
  CGGGGTGATAGAATACTGTGAGAGAGGATCCCTCCGGGAAGTTTTAAATGACACAATTTC
  CTACCCTGATGGCACATTCATGGATTGGGAGTTTAAGATCTCTGTCTTGTATGACATTGC
  TAAGGGAATGTCATATCTGCACTCCAGTAAGACAGAAGTCCATGGTCGTCTGAAATCTAC
  CAACTGCGTAGTGGACAGTAGAATGGTGGTGAAGATCACTGATTTTGGCTGCAATTCCAT
  TTTACCTCCAAAAAAGGACCTGTGGACAGCTCCAGAGCACCTCCGCCAAGCCAACATCTC
  TCAGAAAGGAGATGTGTACAGCTATGGGATCATCGCACAGGAGATCATTCTGCGGAAAGA
  AACCTTCTACACTTTGAGCTGTCGGGACCGGAATGAGAAGATTTTCAGAGTGGAAAATTC
  CAATGGAATGAAACCCTTCCGCCCAGATTTATTCTTGGAAACAGCAGAGGAAAAAGAGCT
  AGAAGTGTACCTACTTGTAAAAAACTGTTGGGAGGAAGATCCAGAAAAGAGACCAGATTT
  CAAAAAAATTGAGACTACACTTGCCAAGATATTTGGACTTTTTCATGACCAAAAAAATGA
  AAGCTATATGGATACCTTGATCCGACGTCTACAGCTATATTCTCGAAACCTGGAACATCT
  GGTAGAGGAAAGGACACAGCTGTACAAGGCAGAGAGGGACAGGGCTGACAGACTTAACTT
  TATGTTGCTTCCAAGGCTAGTGGTAAAGTCTCTGAAGGAGAAAGGCTTTGTGGAGCCGGA
  ACTATATGAGGAAGTTACAATCTACTTCAGTGACATTGTAGGTTTCACTACTATCTGCAA
  ATACAGCACCCCCATGGAAGTGGTGGACATGCTTAATGACATCTATAAGAGTTTTGACCA
  CATTGTTGATCATCATGATGTCTACAAGGTGGAAACCATCGGTGATGCGTACATGGTGGC
  TAGTGGTTTGCCTAAGAGAAATGGCAATCGGCATGCAATAGACATTGCCAAGATGGCCTT
  GGAAATCCTCAGCTTCATGGGGACCTTTGAGCTGGAGCATCTTCCTGGCCTCCCAATATG
  GATTCGCATTGGAGTTCACTCTGGTCCCTGTGCTGCTGGAGTTGTGGGAATCAAGATGCC
  TCGTTATTGTCTATTTGGAGATACGGTCAACACAGCCTCTAGGATGGAATCCACTGGCCT
  CCCTTTGAGAATTCACGTGAGTGGCTCCACCATAGCCATCCTGAAGAGAACTGAGTGCCA
  GTTCCTTTATGAAGTGAGAGGAGAAACATACTTAAAGGGAAGAGGAAATGAGACTACCTA
  CTGGCTGACTGGGATGAAGGACCAGAAATTCAACCTGCCAACCCCTCCTACTGTGGAGAA
  TCAACAGCGTTTGCAAGCAGAATTTTCAGACATGATTGCCAACTCTTTACAGAAAAGACA
  GGCAGCAGGGATAAGAAGCCAAAAACCCAGACGGGTAGCCAGCTATAAAAAAGGCACTCT
  GGAATACTTGCAGCTGAATACCACAGACAAGGAGAGCACCTATTTTTAAACCTAAATGAG
  GTATAAGGACTCACACAAATTAAAATACAGCTGCACTGAGGCAGCGACCTCAAGTGTCCT
  GAAAGCTTACATTTTCCTGAGACCTCAATGAAGCAGAAATGTACTTAGGCTTGGCTGCCC
  TGTCTGGAACATGGACTTTCTTGCATGAATCAGATGTGTGTTCTCAGTGAAATAACTACC
  TTCCACTCTGGAACCTTATTCCAGCAGTTGTTCCAGGGAGCTTCTACCTGGAAAAGAAAA
  GAAATGAATAGACTATCTAGAACTTGAGAAGATTTTATTCTTATTTCATTTATTTTTTGT
  TTGTTTATTTTTATCGTTTTTGTTTACTGGCTTTCCTTCTGTATTCATAAGATTTTTTAA
  ATTGTCATAATTATATTTTAAATACCCATCTTCATTAAAGTATATTTAACTCATAATTTT
  TGCAGAAAATATGCTATATATTAGGCAAGAATAAAAGCTAAAGGTTTCCCAAAAAAAAAA
  AAAAAAAAACTCGACTCTAGATTGCGGCCGCGGTCATAGCTGTTTCCTGAACAGATCCCG
  GGTGGCATCCCTGTGACCCCTCCCCAGTGCCT
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004963 unedited
  AATGTTCTNAAGTGNATGGGGTCAGNAANACTTGCTGTTGGNCTTGGCTTTGTGGTCACT
  GCTCTTCCAGCCCGGGTGGCTGTCCTTTATTCCCAGTGAGTCAGAACTGCCACAATGGCA
  GCTATGAAATCAGCGTCCTGATGATGGGCAACTCAGCCTTTGCAGAGCCCCTGAAAAACT
  TGGAAGATGCGGTGAATGAGGGGCTGGAAATAGTGAGAGGACGTCTGCAAAATGCTGGCC
  TAAATGTGACTGTGAACGCTACTTTCATGTATTCGGATGGTCTGATTCATAACTCAGGCG
  ACTGCCGGAGTAGCACCTGTGAAGGCCTCGACCTACTCAGGAAAATTTCAAATGCACAAC
  GGATGGGCTGTGTCCTCATAGGGCCCTCATGTACATACTCCACCTTCCAGATGTACCTTG
  ACACAGAATTGAGCTACCCCATGATCTCAGCTGGAAGTTTTGGATTGTCATGTGACTATA
  AAGAAACCTTAACCAGGCTGATGTCTCCAGCTAGAAAGTTGATGTACTTCTTGGTTAACT
  TTTGGAAAACCAACGATCTGCCCTTCAAAACTTATTCCTGGAGCACTTCGTATGTTTACA
  AGAATGGTACAGAAACTGAGGACTGTTTCTGGTACCTTAATGCTCTGGAGGCTAGCGTTT
  CCTATTTCTCCCACGAACTCGGCTTTAAGGTGGTGTTAAGACAAGATAAGGAGTTTCAGG
  ATATCTTAATGGACCACAACAGGAAAAGNCATGTGATTATTATGTNGTGGTGGGTCCCAG
  AGTTCCTNNCTACAGCTGAAGGTGACCGAGCAGTGGCTTGAGACATTGTCATTATTCTAG
  TGGATCTTTTCATGACCAGTACTTGGAGGACATGTCACAGCCCCTGACTATATGAAAAAT
  GTCCCTTGTTCTGACGCTGTCTCCTTGGAAATTCCTTCTAATAGCTCTTT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004963 unedited
  NNAATTCTTGTACGCGCCGCTTTCTANATCGAGTTTTTTTTTTTTTTTTTTTGGGAAACC
  TTTAGCTTTTATTCTTGCCTAATATATAGCATATTTTCTGCAAAAATTATGAGTTAAATA
  TACTTTAATGAAGATGGGTATTTAAAATATAATTATGACAATTTAAAAAATCTTATGAAT
  ACAGAAGGAAAGCCAGTAAACAAAAACGATAAATATAAACAAACAAAAAATAAATGAAAT
  AAGAATAAAATCTTCTCAAGTTCTAGATAGTCTATTCATTTCTTTTCTTTTCCAGGTAGA
  AGCTCCCTGGAACAACTGCTGGAATAAGGTTCCAGAGTGGAAGGTAGTTATTTCACTGAG
  AACACACATCTGATTCATGCAAGAAAGTCCATGTTCCAGACAGGGCAGCCAAGCCTAAGT
  ACATTTCTGCTTCATTGAGGTCTCAGGAAAATGTAAGCTTTCAGGACACTTGAGGTCGCT
  GCCTCAGTGCAGCTGTATTTTAATTTGTGTGAGTCCTTATACCTCATTTAGGTTTAAAAA
  TAGGTGCTCTCCTTGTCTGTGGTATTCAGCTGCAAGTATTCCAGAGTGCCTTTTTTATAG
  CTGGCTACCCGTCTGGGTTTTTGGCTTCTTATCCCTGCTGCCTGTCTTTTCTGTAAAGAG
  TTGGCAATCATGTCTGAAAATTCTGCTTGCCAACGCTGTTGATTCTCCACAGTAGGAGGG
  GTTGGCAGGTTGAAATTCTGGTGCTTTCATCCCATCACCCCGTAGGTGATGCCAGTCCCC
  TTCCCTTAAATATGTTCCTCCACTCAATTCGTAAAGGACCGGCACTCAGTTCTCTCAGGA
  GGGTATGGCGGAACCCCTACACTGAATTTCAAAGGGAGGCCAATGGATTCCATCCAAAGG
  CGAGATAACATGT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_004963
• Insert Size: 3900 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004963.1, NP_004954.1
• RefSeq Size: 3745 bp
• RefSeq ORF: 3222 bp
• Locus ID: 2984
• UniProt ID: P25092
• Cytogenetics: 12p12.3
• Protein Families: Druggable Genome, Protein Kinase, Transmembrane
• Protein Pathways: Purine metabolism
• Gene Summary: This gene encodes a transmembrane protein that functions as a receptor for endogenous peptides guanylin and uroguanylin, and the heat-stable E. coli enterotoxin. The encoded protein activates the cystic fibrosis transmembrane conductance regulator. Mutations in this gene are associated with familial diarrhea (autosomal dominant) and meconium ileus (autosomal recessive). [provided by RefSeq, Nov 2016]