LRRC32 (NM_005512) Human Untagged Clone

CAT#: SC116699

LRRC32 (untagged)-Human leucine rich repeat containing 32 (LRRC32), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: LRRC32
• Synonyms: CPPRDD; D11S833E; GARP
• Vector: pCMV6-XL6
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_005512, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGAGACCCCAGATCCTGCTGCTCCTGGCCCTGCTGACCCTAGGCCTGGCTGCACAACACCAAGACAAAG
  TGCCCTGTAAGATGGTGGACAAGAAGGTCTCGTGCCAGGTTCTGGGCCTGCTCCAGGTCCCCTCGGTGCT
  CCCGCCAGACACTGAGACCCTTGATCTATCTGGGAACCAGCTGCGGAGTATCCTGGCCTCACCCCTGGGC
  TTCTACACAGCACTTCGTCACCTGGACCTGAGCACCAATGAGATCAGCTTCCTCCAGCCAGGAGCCTTCC
  AGGCCCTGACCCACCTGGAGCACCTCAGCCTGGCTCACAACCGGCTGGCGATGGCCACTGCGCTGAGTGC
  TGGTGGCCTGGGCCCCCTGCCACGCGTGACCTCCCTGGACCTGTCTGGGAACAGCCTGTACAGCGGCCTG
  CTGGAGCGGCTGCTGGGGGAGGCACCCAGCCTGCATACCCTCTCACTGGCGGAGAACAGTCTGACTCGCC
  TCACCCGCCACACCTTCCGGGACATGCCTGCGCTGGAGCAGCTTGACCTGCATAGCAACGTGCTGATGGA
  CATCGAGGATGGCGCCTTCGAGGGTCTGCCCCGCCTGACCCATCTCAACCTCTCCAGGAATTCCCTCACC
  TGCATCTCCGACTTCAGCCTCCAGCAGCTGCGGGTGCTAGACCTGAGCTGCAACAGCATCGAGGCCTTTC
  AGACGGCCTCCCAGCCCCAGGCTGAGTTCCAGCTCACCTGGCTTGACCTGCGGGAGAACAAACTGCTCCA
  TTTCCCCGACCTGGCCGCGCTCCCGAGACTCATCTACCTGAACTTGTCCAACAACCTCATCCGGCTCCCC
  ACAGGGCCACCCCAGGACAGCAAGGGCATCCACGCACCTTCCGAGGGCTGGTCAGCCCTGCCCCTCTCAG
  CCCCCAGCGGGAATGCCAGCGGCCGCCCCCTTTCCCAGCTCTTGAATCTGGATTTGAGCTACAATGAGAT
  TGAGCTCATCCCCGACAGCTTTCTTGAGCACCTGACCTCCCTGTGCTTCCTGAACCTCAGCAGAAACTGC
  TTGCGGACCTTTGAGGCCCGGCGCTTAGGCTCCCTGCCCTGCCTGATGCTCCTTGACTTAAGCCACAATG
  CCCTGGAGACACTGGAACTGGGCGCCAGAGCCCTGGGGTCTCTGCGGACGCTGCTCCTACAGGGCAATGC
  CCTGCGGGACCTGCCCCCATACACCTTTGCCAATCTGGCCAGCCTGCAGCGGCTCAACCTGCAGGGGAAC
  CGAGTCAGCCCCTGTGGGGGGCCAGATGAGCCTGGCCCCTCCGGCTGTGTGGCCTTCTCCGGCATCACCT
  CCCTCCGCAGCCTGAGCCTGGTGGATAATGAGATAGAGCTGCTCAGGGCAGGGGCCTTCCTCCACACCCC
  ACTGACTGAGCTGGACCTTTCTTCCAATCCTGGGCTGGAGGTGGCCACGGGGGCCTTGGGAGGCCTGGAG
  GCCTCCTTGGAGGTCCTGGCACTGCAGGGCAACGGGCTGATGGTCCTGCAGGTGGACCTGCCCTGCTTCA
  TCTGCCTCAAGCGGCTCAATCTTGCCGAGAACCGCCTGAGCCACCTTCCCGCCTGGACACAGGCTGTGTC
  ACTGGAGGTGCTGGACCTGCGAAACAACAGCTTCAGCCTCCTGCCAGGCAGTGCCATGGGTGGCCTGGAG
  ACCAGCCTCCGGCGCCTCTACCTGCAGGGGAATCCACTCAGCTGCTGCGGCAATGGCTGGCTGGCAGCCC
  AGCTGCACCAGGGCCGTGTGGACGTGGACGCCACCCAGGACCTGATCTGCCGCTTCAGCTCCCAGGAGGA
  GGTGTCCCTGAGCCACGTGCGTCCCGAGGACTGTGAGAAGGGGGGACTGAAGAACATCAACCTCATCATC
  ATCCTCACCTTCATACTGGTCTCTGCCATCCTCCTCACCACGCTGGCCGCCTGCTGCTGCGTCCGCCGGC
  AGAAGTTTAACCAACAGTATAAAGCCTAA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005512 unedited
  TATCCCCCGCCCGTTGNCGCATAGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGC
  AGAGCTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCCGCG
  AATTCGGCACGAGGAGAGGAGCTGCGGCCGCGCTGAGCCAGAGCCATGAGACCCCAGATC
  CTGCTGCTCCTGGCCCTGCTGACCCTAGGCCTGGCTGCACAACACCAAGACAAAGTGCCC
  TGTAAGATGGTGGACAAGAAGGTCTCGTGCCAGGTTCTGGGCCTGCTCCAGGTCCCCTCG
  GTGCTCCCGCCAGACACTGAGACCCTTGATCTATCTGGGAACCAGCTGCGGAGTATCCTG
  GCCTCACCCCTGGGCTTCTACACGGCACTTCGTCACCTGGACCTGAGCACCAATGAGATC
  AGCTTCCTCCAGCCAGGAGCCTTCCAGGCCCTGACCCACCTGGAGCACCTCAGCCTGGCT
  CACAACCGGCTGGCGATGGCCACTGCGCTGAGTGCTGGTGGCCTGGGCCCCCTGCCACGC
  GTGACCTCCCTGGACCTGTCTGGGAACAGCCTGTACAGCGGCCTGCTGGAGCGGCTGCTG
  GGGGAGGCACCCAGCCTGCATACCCTCTCACTGGCGGAGAACAGTCTGACTCGCCTCACC
  CGCCACACCTTCCGGGACATGCCTGCGCTGGAGCAGCTTGACCTGCATAGCAACGTGCTG
  ATGGACATCGAGGATGGCGCCTTCGAGGGCCTGCCCCGCCTGACCCATCTCAACCTCTCC
  AGGAATTCCCTCACCTGCATCTCCGACTTCAGCCTCCAGCAGCTGCGGGTGCTAGACCTG
  AGCTGCACAGCATCGAGGCCTTTCAGACGGCTCCCAGCCCCAGCTGAGTCCAGCTCACCT
  GGCTTGACCTGCGGNAGAACAACTGCTTCATTCC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005512 unedited
  GGAATACTATGNACGCGGCCGCATTCTAGNATCGAGTTTTTTTTTTTTTTTTTTTCTCCA
  AAATTAGTTTTTAATACATTCAGGTAGGTTATACAAAGAGTTGGTCCCGAGGCCTGGGCT
  GAGGCCTGGGCCCTGGGAAGTGACAGCAGCCCTGTGTAGTGTTTTCAAAGATCAGGCGTG
  GGGACCCACACCTTGGCCTCCAGGCCTAAGGAGGAGTGTTACGTGCAGTAGCACCCGTGG
  GTGTGATCCCAACCAGGACTGGGTCTGCCTGGGTGCTCCACACACCACCAGGGCCTAGCC
  CTGGCTCTGCCCATTGACTCTCCCTTAACTTCCTCTGGGCCTTGGTGTTCTCCTCTGTAA
  AATGGGGTGGAGAGAGTGGCATTTTCACTTTCTCGCACATTACTGAGCCCACATCATGAG
  CCAGACTGTGGGCCAAGCACAGGTAAGATGATGAAGACAGCAAAACAAGGTCTTGAGTTG
  GTCCCAGCACGTCCATTCTTCCCAGACACCTCATGACTCCCTGGTATGAGGGATGGGGGT
  CATCTGGGTGAGGCTTTTTACCTGTTTTTGGAAGAGGAAAAAAGATCCTGCCTGATGAGG
  CACAAAAAGGAAGTTTGGAGCCCCCATTATCTTGTGATGTGATAGCTGAGAAAAAGAATC
  ACTTCTGGTGCAAACCCCCAACTTACCCCCAAAAAAGGGGTCTTTTTTCCCCCGAGCTCT
  GTATAAGCTCTCCCCAATTTTAATTTCTTCCTAACCTCAGTTTCCCCATTTTTGCAAGGG
  GGGAGGTGGTTTTCTATATCTTAAATCCAGGCTCTGGTTCTTTTGGGAAAAGGGATGGGA
  GAATCCCGAACCCCTCAGGGACAGGGGAACCCCCCCCAGGGGGTGAAGGGAG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_005512
• Insert Size: 4500 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005512.1, NP_005503.1
• RefSeq Size: 4163 bp
• RefSeq ORF: 1989 bp
• Locus ID: 2615
• UniProt ID: Q14392
• Cytogenetics: 11q13.5
• Domains: LRRNT, LRR, LRR_RI, LRR_TYP, LRR_PS
• Protein Families: Transmembrane
• Gene Summary: This gene encodes a type I membrane protein which contains 20 leucine-rich repeats. Alterations in the chromosomal region 11q13-11q14 are involved in several pathologies. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a). Variants 1-4 all encode the same isoform (a).