EDG7 (LPAR3) (NM_012152) Human Untagged Clone

CAT#: SC115507

LPAR3 (untagged)-Human lysophosphatidic acid receptor 3 (LPAR3)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: EDG7
• Synonyms: Edg-7; EDG7; GPCR; HOFNH30; LP-A3; LPA3; RP4-678I3
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC115507 sequence for NM_012152 edited (data generated by NextGen Sequencing)
  ATGAATGAGTGTCACTATGACAAGCACATGGACTTTTTTTATAATAGGAGCAACACTGAT
  ACTGTCGATGACTGGACAGGAACAAAGCTTGTGATTGTTTTGTGTGTTGGGACGTTTTTC
  TGCCTGTTTATTTTTTTTTCTAATTCTCTGGTCATCGCGGCAGTGATCAAAAACAGAAAA
  TTTCATTTCCCCTTCTACTACCTGTTGGCTAATTTAGCTGCTGCCGATTTCTTCGCTGGA
  ATTGCCTATGTATTCCTGATGTTTAACACAGGCCCAGTTTCAAAAACTTTGACTGTCAAC
  CGCTGGTTTCTCCGTCAGGGGCTTCTGGACAGTAGCTTGACTGCTTCCCTCACCAACTTG
  CTGGTTATCGCCGTGGAGAGGCACATGTCAATCATGAGGATGCGGGTCCATAGCAACCTG
  ACCAAAAAGAGGGTGACACTGCTCATTTTGCTTGTCTGGGCCATCGCCATTTTTATGGGG
  GCGGTCCCCACACTGGGCTGGAATTGCCTCTGCAACATCTCTGCCTGCTCTTCCCTGGCC
  CCCATTTACAGCAGGAGTTACCTTGTTTTCTGGACAGTGTCCAACCTCATGGCCTTCCTC
  ATCATGGTTGTGGTGTACCTGCGGATCTACGTGTACGTCAAGAGGAAAACCAACGTCTTG
  TCTCCGCATACAAGTGGGTCCATCAGCCGCCGGAGGACACCCATGAAGCTAATGAAGACG
  GTGATGACTGTCTTAGGGGCGTTTGTGGTATGCTGGACCCCGGGCCTGGTGGTTCTGCTC
  CTCGACGGCCTGAACTGCAGGCAGTGTGGCGTGCAGCATGTGAAAAGGTGGTTCCTGCTG
  CTGGCGCTGCTCAACTCCGTCGTGAACCCCATCATCTACTCCTACAAGGACGAGGACATG
  TATGGCACCATGAAGAAGATGATCTGCTGCTTCTCTCAGGAGAACCCAGAGAGGCGTCCC
  TCTCGCATCCCCTCCACAGTCCTCAGCAGGAGTGACACAGGCAGCCAGTACATAGAGGAT
  AGTATTAGCCAAGGTGCAGTCTGCAATAAAAGCACTTCCTAA
  
  Clone variation with respect to NM_012152.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_012152 unedited
  GAATTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACCAGAAGAGAGAGAGA
  GCTGGAGAGGAGGCGCTCCGCCCGCCAGCCGCGTCCTCCGCTGCTTCTCCGCGCCCGGCT
  GGAGCCCGGCGCCCGGTCGCCCCGTCGCGCTCGACCCCGAGGGCATGCGGCAGCCGCAGG
  GGCCCCCGCTCCCGGGCTCGGCGGCGCGGGTGAACGTGAGCGGATGTTCACTTCTCCACA
  ATGAATGAGTGTCACTATGACAAGCACATGGACTTTTTTTATAATAGGAGCAACACTGAT
  ACTGTCGATGACTGGACAGGAACAAAGCTTGTGATTGTTTTGTGTGTTGGGACGTTTTTC
  TGCCTGTTTATTTTTTTTTCTAATTCTCTGGTCATCGCGGCAGTGATCAAAAACAGAAAA
  TTTCATTTCCCCTTCTACTACCTGTTGGCTAATTTAGCTGCTGCCGATTTCTTCGCTGGA
  ATTGCCTATGTATTCCTGATGTTTAACACAGGCCCAGTTTCAAAAACTTTGACTGTCAAC
  CGCTGGTTTCTCCGTCAGGGGCTTCTGGACAGTAGCTTGACTGCTTCCCTCACCAACTTG
  CTGGTTATCGCCGTGGAGAGGCACATGTCAATCATGAGGATGCGGGTCCATAGCAACCTG
  ACCAAAAAGAGGGTGACACTGCTCATTTTGCTTGTCTGGGCCATCGCCATTTTTATGGGG
  GCGGTCCCCACACTGGGCTGGAATTGCCTCTGCAACATCTCTGCCTGCTCTTCCCTGGCC
  CNCATTTACAGCANNGAGTACCTTTGNTTCTGGACAGTGTNCACCTCATGCNCTTCTCAT
  CATGGNTGTGGTGACCNTGCGATCTACNTGTACGTCAGAAGAAAACCAAACGTCTGTCTN
  CGCATACAAGTGGGNTCATCAGNNCGCGNAGGACACCATGAGCTATGAANAACGTGATGA
  CTGTCTTAGGGGCGTTTGTGGGATGCTGGACCCNGNCCTGGGGGGTTTGCTCCTCACGGC
  CTAA
  
  3' Read Nucleotide Sequence

  >OriGene 3' genomic read for NM_012152 unedited
  NTTTCTAGGTATACTAGGACCGCGCCGCAATCTAAGATCGCATTTTTTTTTTTTTTTTTT
  TGAAACAAAATCTTGCTCTGTCACCCAAACTAAAGTGCAGTGCTGCGATCTTGGCTCACT
  CTGCAACCTCCACCTCCCGGGTTCAAGTGATTCTTATGCCTCAACTTCCCAAGTACCTGG
  TATTACAGGCATGTGCCACCATGCCTGGCTAGTTTTTGTATTTTTAGTAAAGATGCAATT
  TCACCACATTGCCCAGGCTGGTCTCATACTCCTGGCCTCAAGTGATCCACCTGCCTCAGC
  CTCCCAAAGTGCTGGGATTACAGGCATGAACCAGCGTGCCTGACCTCTCTCCTTGATTCT
  TAACATTGTTCTTTGGTCCTCTTATTCCTCTGTCCTTTTGCTGGGTTTTCTCTACCTGCT
  CCTTAAACGTTGGTGTTCTCTTGGCTTCTTTTGCTCTTACTAGGCAAGATTATCAGCTGG
  ATGTTCCATAGGCACTTCAAACTTCACATTCTCTTCTCCTTGTTTTCTGCCTAAACCCTT
  TACTAACACTCCACTGTTTTTCACCCTCCTGAAGGAAAAATGAAACTTAATGTAGGCTTG
  GCCGAAAGCCCATCTGAGACGGTACCCCCATATCAGAAGTAATCCAAGGGATCCCCGGTT
  TCTTCCCACAGATGCGATAACAACGTCTCCAGATGCCATCACCCAAAATATCTATTAACC
  AACATCCAGTAGACATAGCGTTACTCCAGATACACAATAGCGCTGCCAGAGAACAACCAA
  CAAAGATAAATGAAGAAGAGAGAGGATACCACTCACATATGAACAACCAGAACATGCGGA
  AACGATATAAGGCGGACCGCGGTGCCACCTAAGTTTCACATCGGCACGAAATACCACTAG
  GCCAGCGCGCGGGAGCACGAAGAACGGATGAAAACAG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_012152
• Insert Size: 2750 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_012152.1, NP_036284.1
• RefSeq Size: 1148 bp
• RefSeq ORF: 1062 bp
• Locus ID: 23566
• UniProt ID: Q9UBY5
• Cytogenetics: 1p22.3
• Domains: 7tm_1
• Protein Families: Druggable Genome, GPCR, Transmembrane
• Protein Pathways: Neuroactive ligand-receptor interaction
• Gene Summary: This gene encodes a member of the G protein-coupled receptor family, as well as the EDG family of proteins. This protein functions as a cellular receptor for lysophosphatidic acid and mediates lysophosphatidic acid-evoked calcium mobilization. This receptor couples predominantly to G(q/11) alpha proteins. [provided by RefSeq, Jul 2008]