GTPBP9 (OLA1) (NM_013341) Human Untagged Clone

CAT#: SC115293

OLA1 (untagged)-Human Obg-like ATPase 1 (OLA1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GTPBP9
• Synonyms: DOC45; GBP45; GTBP9; GTPBP9; PTD004
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC115293 sequence for NM_013341 edited (data generated by NextGen Sequencing)
  ATGCCCCCTAAAAAGGGAGGTGATGGAATTAAACCACCCCCAATCATTGGAAGATTTGGA
  ACCTCACTGAAAATTGGTATTGTTGGATTGCCAAATGTTGGGAAATCTACTTTCTTCAAT
  GTGTTAACCAATAGTCAGGCTTCAGCAGAAAACTTCCCGTTCTGCACTATTGATCCTAAT
  GAGAGCAGAGTACCTGTGCCAGATGAAAGGTTTGACTTTCTTTGTCAATACCACAAACCA
  GCAAGCAAAATTCCTGCCTTTCTAAATGTGGTGGATATTGCTGGCCTTGTGAAAGGAGCT
  CACAATGGGCAGGGCCTGGGGAATGCTTTTTTATCTCATATTAGTGCCTGTGATGGCATC
  TTTCATCTAACACGTGCTTTTGAAGATGATGATATCACGCACGTTGAAGGAAGTGTAGAT
  CCTATTCGAGATATAGAAATAATACATGAAGAGCTTCAGCTTAAAGATGAGGAAATGATT
  GGGCCCATTATAGATAAACTAGAAAAGGTGGCTGTGAGAGGAGGAGATAAAAAACTAAAA
  CCTGAATATGATATAATGTGCAAAGTAAAATCCTGGGTTATAGATCAAAAGAAACCTGTT
  CGCTTCTATCATGATTGGAATGACAAAGAGATTGAAGTGTTGAATAAACACTTATTTTTG
  ACTTCAAAACCAATGGTCTACTTGGTTAATCTTTCTGAAAAAGACTACATTAGAAAGAAA
  AACAAATGGTTGATAAAAATTAAAGAGTGGGTGGACAAGTATGACCCAGGTGCTTTGGTC
  ATTCCTTTTAGTGGGGCCTTGGAACTCAAGTTGCAAGAATTGAGTGCTGAGGAGAGACAG
  AAGTATCTGGAAGCGAACATGACACAAAGTGCTTTGCCAAAGATCATTAAGGCTGGGTTT
  GCAGCACTCCAACTAGAATACTTTTTCACTGCAGGCCCAGATGAAGTGCGTGCATGGACC
  ATCAGGAAAGGGACTAAGGCTCCTCAGGCTGCAGGAAAGATTCACACAGATTTTGAAAAG
  GGATTCATTATGGCTGAAGTAATGAAATACGAAGATTTTAAAGAGGAAGGTTCTGAAAAT
  GCAGTCAAGGCTGCTGGAAAGTACAGACAACAAGGCAGAAATTATATTGTTGAAGATGGA
  GATATTATCTTCTTCAAATTTAACACACCTCAACAACCGAAGAAGAAATAA
  
  Clone variation with respect to NM_013341.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_013341 unedited
  GGGTGTCAAATTATGTATACGACTCACTATAGGCGGCCGCGATTCGGCACGAGGGCCGCT
  CGGCGNGAGGGACGGGCCTGCGTTCTCTCCTCCTTCCTCCCCGCCTCCAGCTGCCGGCAG
  GACCTTTCTCTCGCTGCCGCTGGGACCCCGTGTCATCGCCCAGGCCGAGCACGATGCCCC
  CTAAAAAGGGAGGTGATGGAATTAAACCACCCCCAATCATTGGAAGATTTGGAACCTCAC
  TGAAAATTGGTATTGTTGGATTGCCAAATGTTGGGAAATCTACTTTCTTCAATGTGTTAA
  CCAATAGTCAGGCTTCAGCAGAAAACTTCCCGTTCTGCACTATTGATCCTAATGAGAGCA
  GAGTACCTGTGCCAGATGAAAGGTTTGACTTTCTTTGTCAATACCACAAACCAGCAAGCA
  AAATTCCTGCCTTTCTAAATGTGGTGGATATTGCTGGCCTTGTGAAAGGAGCTCACAATG
  GGCAGGGCCTGGGGAATGCTTTTTTATCTCATATTAGTGCCTGTGATGGCATCTTTCATC
  TAACACGTGCTTTTGAAGATGATGATATCACGCACGTTGAAGGAAGTGTAGATCCTATTC
  GAGATATAGAAATAATACATGAAGAGCTTCAGCTTANAGATGAAGAAATGATTGNGCCCA
  TTATAGATAAACTAGAAAAGGTGGCTGTGAGAGGAGGAGATAAAAACTAAAACCTGAATA
  TGATATAATGTGCAAAGTAAAATCCTGGGTTATAGATCAAAAGAAACCTGTTCGCTTCTA
  TCATGATTGGGATGACANAGAGATTGAAGTGTTGAAATAACACTTATTTTTTGACTTCAA
  AACCAATGTTCTACTTGGNTAATCTTTCTGAAAAAGACTACTTTAGAAAGAAAACAAATG
  GTTGATTAAAATTAAGAATGGGTGGACAGTATGACCC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_013341 unedited
  CCCCCCCNNCCCCCCNNNCNCNNCCCCCNNCCCNNCCCCCTTGACTTTGGACCGCGGNCC
  GCATNCTAGGATCGAGTTTTTTTTTTTTTTTTTTGGTTTTGTCAAAGTTTTATTGAGTGT
  AGACATCTGGAGTACTGTAAAACATGCATTATCTGTAGATTCAAAAAGGAGCAAGCCACA
  TTGTCCTCACTGTCAAATGTGTCAGGCTTGGCATACATGATGGAGATTAATGAAGTATCA
  TGAGAGTAATATGGTTCCTGAAAAGCTTCTACAATTTGGAGTAGGGTCTTAATCACGTGA
  AAAGCAAAGCTGTTCACATTTAGTGAACCTGCATTTCATGGGGGGGGNGGGGGGTACACA
  GTATTTTAATTTTAAAACAAATAAAAATAATTTGTTTGTCAAAGATTCCCATCTCCCCAA
  CTTTATTTGTCGCATTGGTTTTCAGAAATTTTAATTTTTTAAAAATCAGATGCCTTTTGG
  AAGTTGTATGTTTATCTGAGCAATAACTAAATTTTATTTCTTCTTCGGTTGTTGAGGTGT
  GTTAAATTTGAAGAAGATAATATCTCCATCTTCAACAATATAATTTCTGCCTTGTTGTCT
  GTACTTTCCAGCAGCCTTGACTGCATTTTCAGAACCTTCCTCTTTAAAATCTTCCTATTT
  CATTACTTCAGCCATAATGAATCCCTTTTCAAAAACTGTGTGAATCTTTCCTGCAGCCTG
  GAGAGCCTTAGCCCCTTCCCTGATGGCCATGCACCCACTTCATCTGGGCCTGCCATGAAA
  AATATTCTAGTTGGAATGCTGCAAACCCCCCCCTAATGGTCCTTTGCAAAACACCTTTGT
  GCCATGTTCCGTTCACAAACCTTCTGTCCTCTCCTTAACACTCAAATTTTTGCAACTTGG
  ATTCCCAAGGCCCCCTTAACAAGAAT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_013341
• Insert Size: 1680 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_013341.3, NP_037473.3
• RefSeq Size: 4356 bp
• RefSeq ORF: 1191 bp
• Locus ID: 29789
• UniProt ID: Q9NTK5
• Cytogenetics: 2q31.1
• Gene Summary: This gene encodes a member of the GTPase protein family. The encoded protein interacts with breast cancer-associated gene 1 (BRCA1) and BRCA1-associated RING domain protein (BARD1), and is involved in centrosome regulation. Overexpression of this gene has been observed in multiple types of cancer and may be associated with poor survival. Pseudogenes of this gene have been defined on chromosomes 17 and 22. [provided by RefSeq, Jun 2016]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.