NCF4 (NM_013416) Human Untagged Clone

CAT#: SC115259

NCF4 (untagged)-Human neutrophil cytosolic factor 4, 40kDa (NCF4), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: NCF4
• Synonyms: CGD3; NCF; P40PHOX; SH3PXD4
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_013416, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCTGTGGCCCAGCAGCTGCGGGCCGAGAGTGACTTTGAACAGCTTCCGGATGATGTTGCCATCTCGG
  CCAACATTGCTGACATCGAGGAGAAGAGAGGCTTCACCAGCCACTTTGTTTTCGTCATCGAGGTGAAGAC
  AAAAGGAGGATCCAAGTACCTCATCTACCGCCGCTACCGCCAGTTCCATGCTTTGCAGAGCAAGCTGGAG
  GAGCGCTTCGGGCCAGACAGCAAGAGCAGTGCCCTGGCCTGTACCCTGCCCACACTCCCAGCCAAAGTCT
  ACGTGGGTGTGAAACAGGAGATCGCCGAGATGCGGATACCTGCCCTCAACGCCTACATGAAGAGCCTGCT
  CAGCCTGCCGGTCTGGGTGCTGATGGATGAGGACGTCCGGATCTTCTTTTACCAGTCGCCCTATGACTCA
  GAGCAGGTGCCCCAGGCACTCCGCCGGCTCCGCCCGCGCACCCGGAAAGTCAAGAGCGTGTCCCCACAGG
  GCAACAGCGTTGACCGCATGGCAGCTCCGAGAGCAGAGGCTCTATTTGACTTCACTGGAAACAGCAAACT
  GGAGCTGAATTTCAAAGCTGGAGATGTGATCTTCCTCCTCAGTCGGATCAACAAAGACTGGCTGGAGGGC
  ACTGTCCGGGGAGCCACGGGCATCTTCCCTCTCTCCTTCGTGAAGATCCTCAAAGACTTCCCTGAGGAGG
  ACGACCCCACCAACTGGCTGCGTTGCTACTACTACGAAGACACCATCAGCACCATCAAGTCTGTGGCCTG
  GGAGGGAGGGGCCTGTCCAGCCTTCCTGCCATCCCTACGACCACTGCCCCTCACATCACCTTCTCATGGG
  TCCCTCTCCCACTCCAAAGCCCCCAGTGGCTCCCAGATGAGCCACAATGCTGTAACAAGCCATCAACGTC
  CAGGGTGGCCTGGCCAGCCTCATTCCCCTTTCCCCCACCCCACACCCCACTTCCAGCCTGATGCCTCCTT
  ACTCCAGCCTGTCACCCCCTTAGGGACATCGCGGTGGAGGAAGATCTCAGCAGCACTCCCCTATTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_013416 unedited
  CCCAATTTGTAATACGAACTCACTATAGGGCGGCCGCGAATTCGCACGAGGGGGACTCCC
  AGACCACAGCTGAGACGAGACGCAGGGTGGCTGGAGGAAGTGAGAGGTGAACTCAGCCTG
  GGACTGGCTGGGCGAGACTCTCCACCTGCTCCCTGGGACCATCGCCCACCATGGCTGTGG
  CCCAGCAGCTGCGGGCCGAGAGTACCTTTGAACAGCTTCCGGATGATGTTGCCATCTCGG
  CCAACATTGCTGACATCGAGGAGAAGAGAGGCTTCACCAGCCACTTTGTTTTCGTCATCG
  AGGTGAAGACAAAAGGAGGATCCAAGTACCTCATCTACCGCCGCTACCGCCAGTTCCATG
  CTTTGCAGAGCAAGCTGGAGGAGCGCTTCGGGCCAGACAGCAAGAGCAGTGCCCTGGCCT
  GTACCCTGCCCACACTCCCAGCCAAAGTCTACGTGGGTGTGAAACAGGAGCTCGCCGAGA
  TGCGGATACCTGCCCTCAACGCCTACATGAAGAGCCTGCTCAGCCTGCCGGTCTGGGTGC
  TGATGGATGAGGACGTCCGGATCTTCTTTTACCAGTCGCCCTATGACTCAGAGCAGGTGC
  CCCAGGCACTCCGCCGGCTCCGCCCGCGCACCCGGAAAGTCAAGAGCGTGTCCCCACAGG
  GCAACAGCGTTGACCGCATGGCAGCTCCGAGAGCAGAGGCTCTATTTGACTTCACTGGAA
  ACAGCAAACTGGAGCTGAATTTCAAAGCTGGAGATGTGATCTTCCTCCTCAGTCCGATCA
  ACAAAGACTGGCTGGAGGGCACTGTTCCGGGAGCCACGGGCATCTTCCCTCTCCCCTTCG
  TGAAGACCTCAAAGACTTCCCCTGAGAGGACGACCCCACCACTGGCTGCGTTGCTCTACA
  CGAAGACACATNGGACCATCAGGACATGCGTGGAGGAGTCTCCCAGCACTCCCTATGAAG
  ACTGCTGGACTACAGG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_013416 unedited
  GGGGGGGCCGGGCCCGNGCCCCCTTCNNNNNGTNNGTACTTGNACGCGGCCGCTTTTNAN
  NGATCGGTTTTTTTTTTTTTTTTTTTTTTTTTCAAATGGATGGTTTATTTAAATCAGATG
  TAAATAGTAGAAAGAAGAAGATTACAAGCCCCACGGGTCCATTAGCCTCAAAAACAGGAA
  GTCTGCCCATCCTCCCAGGTTTTCCTGGTCCCAATCTCCTCTGAGAGCTGAAGGTTTTTG
  CTGGTGTCCCCTCACTGCTCCAGGGACACCGTCAGCTCATGGCATCGTGTTGTAAACCCT
  GTAGTTGTCCTTCTGCGTGATGTGCAGCTTCCAGGGGAAGAGGCGCTTCTGGGAGGGGAG
  GCCACGAGCCTGCCGCACCATGAGCGCTACGTCCTCATCCGACAGCAGCCGAACCAGATC
  CCCCTCAGCGTCCCGGTAATTCAGAGCTATGTCCTCTCTCTGGAACTCCCGCCTTGTGAG
  CTCCAGCAGGTCTTTCAATAGGGGAGTGCTGCTGAGATCTTCCTCCACCGCGATGTCCTT
  GATGGTGCTGATGGTGTCTTCGTAGTAGTAGCAACGCAGCCAGTTGGTGGGGTCGTCCTC
  CTCAGGGAAGTCTTTGAGGATCTTCACGAAGGAGAGAGGGAAGATGCCCGTGGCTCCCCG
  GACAGTGCCCTCCAGCCAGTCTTTGTTGATCCGACTGAGGAGGAAGATCACATTTCCAGC
  TTTGAAATTCAGCTCCAGTTTGCTGTTTCCAGTGAAGCCAAATGAGCCTCTGCTCTCGGC
  CTGCCATGCCGTCAACGCTGTTGCCCTGGGGGGACCCCTTTTTGACTTTCCCGGTGCCNC
  GGCCGGAACCGGCCGAATGCCCTGGGGCACCTGCTTTGGATTATAAGGCGACTGGTAAAA
  AAAGAACCGGACGTCCTCATCCTTAG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_013416
• Insert Size: 1470 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_013416.2, NP_038202.1
• RefSeq Size: 1631 bp
• RefSeq ORF: 1047 bp
• Locus ID: 4689
• UniProt ID: Q15080
• Cytogenetics: 22q12.3
• Domains: SH3, PX
• Protein Pathways: Leukocyte transendothelial migration
• Gene Summary: The protein encoded by this gene is a cytosolic regulatory component of the superoxide-producing phagocyte NADPH-oxidase, a multicomponent enzyme system important for host defense. This protein is preferentially expressed in cells of myeloid lineage. It interacts primarily with neutrophil cytosolic factor 2 (NCF2/p67-phox) to form a complex with neutrophil cytosolic factor 1 (NCF1/p47-phox), which further interacts with the small G protein RAC1 and translocates to the membrane upon cell stimulation. This complex then activates flavocytochrome b, the membrane-integrated catalytic core of the enzyme system. The PX domain of this protein can bind phospholipid products of the PI(3) kinase, which suggests its role in PI(3) kinase-mediated signaling events. The phosphorylation of this protein was found to negatively regulate the enzyme activity. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (2) represents the longer transcript and encodes the longer isoform (2). This isoform (2) lacks the PC (Phox and Cdc24p) motif, which may be important for the interaction with NCF2.