DUSP16 (NM_030640) Human Untagged Clone

CAT#: SC111890

DUSP16 (untagged)-Human dual specificity phosphatase 16 (DUSP16)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DUSP16
• Synonyms: MKP-7; MKP7
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC111890 sequence for NM_030640 edited (data generated by NextGen Sequencing)
  ATGGCCCATGAGATGATTGGAACTCAAATTGTTACTGAGAGGTTGGTGGCTCTGCTGGAA
  AGTGGAACGGAAAAAGTGCTGCTAATTGATAGCCGGCCATTTGTGGAATACAATACATCC
  CACATTTTGGAAGCCATTAATATCAACTGCTCCAAGCTTATGAAGCGAAGGTTGCAACAG
  GACAAAGTGTTAATTACAGAGCTCATCCAGCATTCAGCGAAACATAAGGTTGACATTGAT
  TGCAGTCAGAAGGTTGTAGTTTACGATCAAAGCTCCCAAGATGTTGCCTCTCTCTCTTCA
  GACTGTTTTCTCACTGTACTTCTGGGTAAACTGGAGAAGAGCTTCAACTCTGTTCACCTG
  CTTGCAGGTGGGTTTGCTGAGTTCTCTCGTTGTTTCCCTGGCCTCTGTGAAGGAAAATCC
  ACTCTAGTCCCTACCTGCATTTCTCAGCCTTGCTTACCTGTTGCCAACATTGGGCCAACC
  CGAATTCTTCCCAATCTTTATCTTGGCTGCCAGCGAGATGTCCTCAACAAGGAGCTGATG
  CAGCAGAATGGGATTGGTTATGTGTTAAATGCCAGCAATACCTGTCCAAAGCCTGACTTT
  ATCCCCGAGTCTCATTTCCTGCGTGTGCCTGTGAATGACAGCTTTTGTGAGAAAATTTTG
  CCGTGGTTGGACAAATCAGTAGATTTCATTGAGAAAGCAAAAGCCTCCAATGGATGTGTT
  CTAGTGCACTGTTTAGCTGGGATCTCCCGCTCCGCCACCATCGCTATCGCCTACATCATG
  AAGAGGATGGACATGTCTTTAGATGAAGCTTACAGATTTGTGAAAGAAAAAAGACCTACT
  ATATCTCCAAACTTCAATTTTCTGGGCCAACTCCTGGACTATGAGAAGAAGATTAAGAAC
  CAGACTGGAGCATCAGGGCCAAAGAGCAAACTCAAGCTGCTGCACCTGGAGAAGCCAAAT
  GAACCTGTCCCTGCTGTCTCAGAGGGTGGACAGAAAAGCGAGACGCCCCTCAGTCCACCC
  TGTGCCGACTCTGCTACCTCAGAGGCAGCAGGACAAAGGCCCGTGCATCCCGCCAGCGTG
  CCCAGCGTGCCCAGCGTGCAGCCGTCGCTGTTAGAGGACAGCCCGCTGGTACAGGCGCTC
  AGTGGGCTGCACCTGTCCGCAGACAGGCTGGAAGACAGCAATAAGCTCAAGCGTTCCTTC
  TCTCTGGATATCAAATCAGTTTCATATTCAGCCAGCATGGCAGCATCCTTACATGGCTTC
  TCCTCATCAGAAGATGCTTTGGAATACTACAAACCTTCCACTACTCTGGATGGGACCAAC
  AAGCTATGCCAGTTCTCCCCTGTTCAGGAACTATCGGAGCAGACTCCCGAAACCAGTCCT
  GATAAGGAGGAAGCCAGCATCCCCAAGAAGCTGCAGACCGCCAGGCCTTCAGACAGCCAG
  AGCAAGCGATTGCATTCGGTCAGAACCAGCAGCAGTGGCACCGCCCAGAGGTCCCTTTTA
  TCTCCACTGCATCGAAGTGGGAGCGTGGAGGACAATTACCACACCAGCTTCCTTTTCGGC
  CTTTCCACCAGCCAGCAGCACCTCACGAAGTCTGCTGGCCTGGGCCTTAAGGGCTGGCAC
  TCGGATATCTTGGCCCCCCAGACCTCTACCCCTTCCCTGACCAGCAGCTGGTATTTTGCC
  ACAGAGTCCTCACACTTCTACTCTGCCTCAGCCATCTACGGAGGCAGTGCCAGTTACTCT
  GCCTACAGCTGCAGCCAGCTGCCCACTTGCGGAGACCAAGTCTATTCTGTGCGCAGGCGG
  CAGAAGCCAAGTGACAGAGCTGACTCGCGGCGGAGCTGGCATGAAGAGAGCCCCTTTGAA
  AAGCAGTTTAAACGCAGAAGCTGCCAAATGGAATTTGGAGAGAGCATCATGTCAGAGAAC
  AGGTCACGGGAAGAGCTGGGGAAAGTGGGCAGTCAGTCTAGCTTTTCGGGCAGCATGGAA
  ATCATTGAGGTCTCCTGA
  
  Clone variation with respect to NM_030640.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_030640 unedited
  CGCGTGGCGNATTTGTATACGATTCCTATAGGCGGCCCGCGAATTCGCACGAGGGGTCGG
  CGGNCCCCAAAAGCTTTCAGTCCAGTGTAAAGCTGTTGGAGCGCGGGAGCAAAGGTAAAG
  AATGATGTAATGCGCTGGCTGCTCCAAAGCATCTTTTGTTGTGGAATGGTTATTCCAGTC
  ATCTCTTTATGAATCAAATGTGAGGGGCTGCTTTGTGGACGGAGTCCTTTGCAAGAGCAC
  ATCAACGGGAAAGAGAAAGAGACATTCACTTGGAGGGCTCTTGCTGAAAATGGGTTTAAC
  TCTCCTTTTGCCAGTCACCACCAGCCTGACCTCATACACTTTTAGTACAATGGAGTGGCT
  GAGCCTTTGAGCACACCACCATTACATCATCGTGGCAAATTAAAGAAGGAGGTGGGAAAA
  GAGGACTTATTGTTGTCATGGCCCATGAGATGATTGGAACTCAAATTGTTACTGAGAGGT
  TGGTGGCTCTGCTGGAAAGTGGAACGGAAAAAGTGCTGCTAATTGATAGCCGGCCATTTG
  TGGAATACAATACATCCCACATTTTGGAAGCCATTAATATCAACTGCTCCAAGCTTATGA
  AGCGAAGGTTGCAACAGGACAAAGTGTTAATTACAGAGCTCATCCAGCATTCAGCGAAAC
  ATAAGGTTGACATTGATTGCAGTCAGAAGGTTGTAGTTTACGATCAAAGCTCCCAAGATG
  TTGCCTCTCTCTCTTCAGACTGTTTTCTCACTGTACTTCTGGGTAAACTGGAGAAGAGCT
  TCAACTCTGTTCACCTGCTTGCAGGTGGGTTTGCTGAGTTCTCTCNGTGTTTCCCTGGCC
  TCTGTGAAGGAAAATCCACTCTAGTCCCTACCTGCATTTCTCAGCCTTGCTTACCTGNNT
  GCCACATTGNGCCCACCCGNNATCT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_030640 unedited
  NCCTTTAGCTATGNNACCGCGGCCGCTATNCTAGNGATCGAGCTTTTTTTTTTTTTTTTT
  TTTTTTTTTTTTTGAAACCAAGTTCATCTTTATTTAAAGGATTGACAATCCCATTTTAAA
  CAATTCTTTGATTTACAAAGAGGGAGGTAGACTCGTTAGCCTCCCAACCTTAGCTTAAAT
  CGTGATGTTGCCAGGTTCCTGGTGGTTCAGCTGAATCCTAAACAGTTTCCCTTCTCTTGA
  TAAAGCTGAGAAGAAAAAAAAATTATCTCGATCTAGGCCCACGGGAATTTTGTGCATAGA
  CAGCTTGAATTGGCCTGAAAAGTGCGACTACTTTCCTCCCTATCCCCACTGGCTTACATC
  TGGGCCCACCCCACTCTCCGCCGCTGTATTCGCCTGCTATTTCCTTCCTCCTTCTCCCTC
  CTCTCTTCTTTTTCCTCCTCATTTCCTTTCCGTCCTTTTTTTCGTTGCCCTCTCCCTCCC
  TCCCCCAAACCCCCCTTTTCCCCCCTGCTCCCTCCCCTCCCCCCCTTCCTCCCTTCGCGC
  CCTCCCCCTCCCCCCCACCTTTATTCCCCCACCTCTCGCTCCTCCTCCTCCTCTCGCCTC
  TTCCTATTCCTCATGTTCCCCGACTTCCCCCCCCCCCACTCCTTTCCTCTTCCCTCTCGC
  CTGCTCCTCCCGTTTTCTCGTCCTTTGATCCTCCTCCCCATCCGCTCCATCCGCCAACCC
  TTTAACTTGTCCTCGTTTTTTTCCCCCCTCCAACCTTCCTCCTCTCTTCCTCCCCCTCAC
  CCCCCTCTTTACCCCGTTCATCTCCCATCACGCCCCCGTCCTTCCCTCCCTCTCTTCCCC
  TCACCTCGCCCCCCCCCTCCCCCCTCTCCCCCTCACCCGCCCCGCCACCTCCCCTCCCTC
  CCTCCCCCCCTCCCACCCCCCTCTCTCCTCTCTTTCCCCCCCCCCCTCTCTCTCCCGCTC
  TTTCCTCCCCCCACTCCNCAC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_030640
• Insert Size: 3410 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_030640.1, NP_085143.1
• RefSeq Size: 3566 bp
• RefSeq ORF: 1998 bp
• Locus ID: 80824
• UniProt ID: Q9BY84
• Cytogenetics: 12p13.2
• Protein Families: Druggable Genome, Phosphatase
• Protein Pathways: MAPK signaling pathway
• Gene Summary: This gene encodes a mitogen-activated protein kinase phosphatase that is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. The encoded protein specifically regulates the c-Jun amino-terminal kinase (JNK) and extracellular signal-regulated kinase (ERK) pathways.[provided by RefSeq, May 2010]