ZUP1 (NM_145062) Human Untagged Clone

CAT#: SC100069

ZUFSP (untagged)-Human zinc finger with UFM1-specific peptidase domain (ZUFSP)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ZUP1
• Synonyms: C6orf113; DUB; ZUFSP
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC100069 sequence for NM_145062 edited (data generated by NextGen Sequencing)
  ATGCTTTCCTGTAATATTTGTGGTGAAACAGTAACCTCAGAACCAGACATGAAAGCTCAC
  CTAATTGTTCACATGGAAAGTGAAATTATATGTCCATTTTGCAAGTTGTCAGGTGTGAAT
  TATGATGAAATGTGTTTTCATATCGAAACAGCTCATTTTGAGCAGAATACACTTGAAAGA
  AACTTTGAGAGGATAAATACAGTACAATATGGAACTTCAGATAACAAGAAAGACAACACC
  CTACAGTGTGGAATGGAAGTTAATTCAAGTATTCTTTCAGGTTGTGCATCTAATCATCCA
  AAAAATTCAGCTCAAAACCTGACTAAAGATAGTACTTTAAAACATGAAGGCTTCTATTCA
  GAGAACTTAACTGAATCTAGAAAATTCCTGAAAAGTAGGGAAAAACAGTCCAGCCTGACC
  GAAATAAAAGGATCTGTTTATGAAACAACATACAGTCCTCCTGAATGTCCATTCTGTGGA
  AAAATAGAGGAGCACAGTGAAGATATGGAAACTCATGTGAAAACAAAGCATGCCAATCTT
  TTAGACATTCCATTGGAAGACTGTGATCAACCACTCTATGATTGTCCTATGTGTGGGCTC
  ATATGTACAAATTACCATATTCTTCAGGAACATGTTGACTTGCATTTGGAAGAAAACAGC
  TTTCAGCAAGGCATGGATAGAGTCCAGTGTTCTGGTGATCTACAATTGGCTCACCAGCTT
  CAGCAAGAAGAAGACAGAAAGAGGAGATCTGAAGAATCAAGACAAGAAATAGAAGAATTT
  CAGAAGCTGCAGAGACAATATGGTTTAGATAATTCTGGAGGATACAAACAACAACAACTA
  CGAAATATGGAGATAGAAGTAAATAGGGGAAGAATGCCTCCATCTGAATTTCATAGGAGA
  AAAGCTGATATGATGGAATCATTAGCTCTTGGTTTTGACGATGGAAAAACAAAAACTTCC
  GGAATTATTGAAGCACTTCATAGGTATTATCAGAATGCTGCCACAGATGTGAGACGGGTG
  TGGCTTTCTTCAGTGGTGGATCACTTTCATTCATCTTTAGGCGACAAAGGTTGGGGTTGT
  GGTTACAGAAATTTCCAAATGCTACTTTCATCATTATTACAAAATGATGCTTACGACGAT
  TGCTTAAAAGGTATGTTGATTCCTTGCATTCCAAAAATTCAATCTATGATTGAAGATGCA
  TGGAAGGAAGGTTTTGATCCTCAGGGGGCCTCTCAACTTAATAACAGGTTACAGGGAACA
  AAGGCCTGGATTGGAGCATGTGAAGTATATATACTCCTGACCTCCCTAAGGGTAAAGTGT
  CATATTGTTGATTTTCACAAATCAACTGGTCCTTTGGGTACACACCCTCGCTTATTTGAA
  TGGATATTGAACTATTATTCTTCAGAGGGAGAAGGGAGTCCAAAGGTAGTGTGTACATCT
  AAACCTCCTATCTATCTTCAGCATCAAGGTCACAGTCGAACTGTTATTGGAATTGAAGAG
  AAAAAAAACCGAACATTATGCTTACTAATACTTGATCCTGGATGTCCTTCTCGAGAAATG
  CAGAAATTATTAAAGCAAGACATAGAGGCTAGCAGTCTCAAGCAACTTCGGAAATCTATG
  GGAAATTTAAAACATAAGCAATACCAGATATTGGCAGTAGAGGGTGCTCTTTCTCTAGAG
  GAGAAACTTGCCAGGAGACAAGCTTCTCAAGTCTTTACAGCCGAGAAGATTCCTTGA
  
  Clone variation with respect to NM_145062.2
  1135 a=>g
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_145062 unedited
  GGATTTTGTAATACGACTTACTATAGGGCGGCCGCGAATTCGGCACGAGGGCCAAGTGTT
  GGAAGTTCCTAATTCTTTCCTCGGTTAACTGTGAAACTCTGCGTATTGGGAAGGCCTGGC
  CTCAGTCATCAGGCCAGGAGAGGTACTGGACGCCGCGCACGCACTCGTCTGCCAGCGAGG
  CCCAAAGGGGAAGCCTAGCGGAGCTCAGTGTGGCAGCTGCTGGCCTCTGGGCCGCTACTT
  GTCAATACCATGCTTTCCTGTAATATTTGTGGTGAAACAGTAACCTCAGAACCAGACATG
  AAAGCTCACCTAATTGTTCACATGGAAAGTGAAATTATATGTCCATTTTGCAAGTTGTCA
  GGTGTGAATTATGATGAAATGTGTTTTCATATCGAAACAGCTCATTTTGAGCAGAATACA
  CTTGAAAGAAACTTTGAGAGGATAAATACAGTACAATATGGAACTTCAGATAACAAGAAA
  GACAACACCCTACAGTGTGGAATGGAAGTTAATTCAAGTATTCTTTCAGGTTGTGCATCT
  AATCATCCAAAAAATTCAGCTCAAAACCTGACTAAAGATAGTACTTTAAAACATGAAGGC
  TTCTATTCAGAGAACTTAACTGAATCTAGAAAATTCCTGAAAAGTAGGGAAAAACAGTCC
  AGCCTGACCGAAATAAAAGGATCTGTTTATGAAACAACATACAGTCCTCCTGAATGTCCA
  TTCTGTGGAAAAATAGAGGAGCACAGTGAAGATATGGAAACTCATGTGAAACAAAGCATG
  CCAATCTTTTAGACATTCCATTGGAAGACTGTGATCAACCACTCTATGATTGNCCTATGT
  GTGGGCTCATATGTACAAAATACATATTCTTCAGAACATGTTGACTTGCATTTGGAAGAA
  ACAGCTTCANCAGGCTGGATAGAGTCATGGTCCTGTGATCTACATGGGCTACAGCTCACA
  GAGAGACGANGGAGGGTCTGAGATCAGAAGAATGAGAA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_145062 unedited
  GTAAATCTATGNNACCGCGGCCCGCATTTTANGATCGAGTTTTTTTTTTTTTTTTTTTGC
  AAAAACAAGACATTTTATTGAAATAATTTACCAAACAATGAAGTATGATAGGTATAATTC
  AATTAACACAGGCATTTTAGAAATTAAATTATATGTTAAGACTTAAGAGAATTCACAGAT
  TCATAATTGTATTAAGGAGTTCAATATCTACATTTAGAAAACAAAGTATTGTTCTCAATC
  ACTGAAATGCTTAAATGCTTGATTCTTCAAGGAATCTTCTCGGCTGTAAAGACTTGAGAA
  GCTTGTCTCCTGGCAAGTTTCTCCTCTAGAGAAAGAGCACCCTCTACTGCCAATATCTGG
  TATTGCTTATGTTTTAAATTTCCCATAGATTTCCGAAGTTGCTTGAGACTGCTAGCCTCT
  ATGTCTTGCTTTAATAATTTCTGCATTTCTCGAGAAGGACATCCAGGATCAAGTATTAGT
  AAGCATAATGTTCGGTTTTTTTTCTCTTCAATTCCAATAACAGTTCGACTGTGACCTTGA
  TGCTGAAGATAGATAGGAGGTTTAGATGTACACACTACCTTTGGACTCCCTTCTCCCTCT
  GAAGAATAATAGTTCAATATCCATTCAAATAAGCGAGGGTGTGTACCCAAAGGACCAGTT
  GATTTGTGAAAATCAACAATATGACACTTTACCCTTAGGGAGGTCAGGAGTATATATACT
  TCACATGCTCCAATCCAGGCCTTTGATTCCCTGTAACCTGTTATTAAGTTGAGAGGCCCC
  CCTGAGATCAAAACCTTCCTTCCATGCATCTTTCATCATAGATTGAATTTTTGGAATGCA
  AGGAATCAACATACCTTTTAAAGCATCGTCGTAGCATCATTTTTGAATATGATGAAAGTG
  GCATTTGGGAAATTTTGTACCACAACCC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_145062
• Insert Size: 2200 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_145062.1, NP_659499.1
• RefSeq Size: 2197 bp
• RefSeq ORF: 1737 bp
• Locus ID: 221302
• UniProt ID: Q96AP4
• Cytogenetics: 6q22.1
• Domains: zf-C2H2
• Gene Summary: This gene encodes a protein containing zinc finger motifs and a cysteine peptidase domain. The encoded protein functions as a K63-specific de-ubiquitinating enzyme that specifically cleaves long K63-linked polyubiquitin chains in the middle of a chain (i.e. "endo cleavage) rather than by removing the terminal ubiquitin from a chain. This enzyme is thought to be involved in the regulation of DNA repair by cleaving K63-linked ubiquitin chains at repair foci. This protein is related to proteases for the ubiquitin-like modifiers Ufm1 (ubiquitin fold modifier 1) and Atg8/Gabarapl2, but does not have any activity on these modifiers. [provided by RefSeq, Mar 2018]