Cxcr3 (NM_053415) Rat Tagged ORF Clone

CAT#: RR210161

Cxcr3 (Myc-DDK-tagged ORF) - Rat chemokine (C-X-C motif) receptor 3 (Cxcr3), (10 ug)

Product Images

Circular map for RR210161

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Cxcr3
• Synonyms: Gpr9
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR210161 representing NM_053415
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTACCTTGAGGTCAGTGAACGTCAAGTGCTAGATGCCTCGGACATTGCCTTTCTTCTGGAAAACAGCA
  CATCTCCCTACGATTATGGGGAAAACGAAAGCGACTTCTCTGACTCCCCGCCCTGCCCACAGGACTTCAG
  CCTGAACTTTGACAGAACCTTCCTGCCAGTCCTCTACAGCCTCCTCTTTTTGCTGGGGCTGCTAGGCAAT
  GGGGCAGTGGCTGCTGTGCTACTGAGCCAGCGCACTGCCCTGAGCAGCACAGACACCTTCCTGCTCCACC
  TGGCTGTGGCCGATGTACTGCTGGTACTAACCCTCCCACTGTGGGCAGTAGATGCTGCTGCCCAGTGGGT
  TTTCGGCTCTGGTCTCTGCAAAGTGGCAGGTGCCCTGTTCAACATCAACTTCTACGCAGGGGCCTTCCTG
  CTGGCCTGTATAAGCTTTGACCGCTACCTGAGCATAGTGCATGCCACCCAGATCTACCGCAGGGACCCCT
  GGGTACGTGTAGCCCTCACCTGCATTGTTGTGTGGGGTCTCTGTGTGCTCTTTGCCCTCCCAGATTTCAT
  CTTCCTGTCAGCCAGCCATGATCAGCGCCTCAATGCCACCCATTGCCAGTACAACTTCCCACAGGTGGGT
  CGGACTGCTCTGCGTGTACTGCAGCTAGTGGCTGGTTTCCTGATGCCCCTTCTAGTCATGGCCTACTGCT
  ATGCCCATATCCTGGCTGTGCTGCTGGTCTCCAGAGGCCAGAGGCGCTTTCGAGCTATGAGGCTAGTGGT
  GGTGGTGGTGGTGGCCTTTGCCGTCTGCTGGACCCCCTATCACCTGGTGGTGTTAGTGGATATCCTTATG
  GACGTGGGAGTTTTGGCCCGCAACTGTGGTCGAGAAAGCCATGTGGACGTAGCCAAGTCAGTCACCTCAG
  GCATGGGCTACATGCACTGCTGCCTCAATCCACTGCTCTATGCCTTTGTGGGAGTGAAGTTCAAAGAACA
  AATGTGGATGTTGCTCATGCGCCTGGGCCGCTCTGACCAGAGAGGACCGCAGCGGCAGCCTTCATCTTCA
  CGGAGAGAATCATCCTGGTCTGAGACAACTGAGGCCTCCTACTTGGGCTTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR210161 representing NM_053415
  Red=Cloning site Green=Tags(s)
  
  MYLEVSERQVLDASDIAFLLENSTSPYDYGENESDFSDSPPCPQDFSLNFDRTFLPVLYSLLFLLGLLGN
  GAVAAVLLSQRTALSSTDTFLLHLAVADVLLVLTLPLWAVDAAAQWVFGSGLCKVAGALFNINFYAGAFL
  LACISFDRYLSIVHATQIYRRDPWVRVALTCIVVWGLCVLFALPDFIFLSASHDQRLNATHCQYNFPQVG
  RTALRVLQLVAGFLMPLLVMAYCYAHILAVLLVSRGQRRFRAMRLVVVVVVAFAVCWTPYHLVVLVDILM
  DVGVLARNCGRESHVDVAKSVTSGMGYMHCCLNPLLYAFVGVKFKEQMWMLLMRLGRSDQRGPQRQPSSS
  RRESSWSETTEASYLGL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_053415
• ORF Size: 1101 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_053415.1, NP_445867.1
• RefSeq Size: 1678 bp
• RefSeq ORF: 1104 bp
• Locus ID: 84475
• UniProt ID: Q9JII9
• Cytogenetics: Xq22
• MW: 40.9 kDa
• Gene Summary: binds interferon-inducible protein-10 and induces intracellular calcium mobilization; increased expression occurs in response to focal stroke [RGD, Feb 2006]