Grik4 (NM_012572) Rat Tagged ORF Clone

CAT#: RR208922

Grik4 (Myc-DDK-tagged ORF) - Rat glutamate receptor, ionotropic, kainate 4 (Grik4), (10 ug)

Product Images

Circular map for RR208922

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Grik4
• Synonyms: GluK4; KA1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR208922 representing NM_012572
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCCCGTGTCTCTGCTCCTCTGGTGCTGCTTCCTGCTTGGCTCTTGATGGTGGCCTGTAGCCCACACT
  CCCTGAGGATCGCTGCTATCTTGGATGACCCCATGGAGTGCAGCAGAGGGGAACGGCTCTCCATCACCCT
  GGCCAAGAACCGCATCAACCGCGCACCAGAGAGGCTGGGCAAGGCAAAAGTCGAAGTGGACATCTTTGAG
  CTTCTCAGAGACAGCGAGTACGAAACTGCAGAAACCATGTGTCAGATCCTCCCCAAGGGAGTGGTCGCTG
  TCCTGGGACCGTCGTCCAGCCCAGCCTCCAGCTCCATCATCAGCAACATCTGTGGAGAGAAGGAGGTCCC
  TCATTTCAAAGTGGCCCCAGAGGAGTTTGTCAGGTTCCAACTCCAGAGATTCACCACCCTGAACCTTCAC
  CCCAGCAACACCGACATCAGCGTGGCTGTGGCTGGGATCCTGAACTTCTTCAACTGCACCACCGCCTGCC
  TCATCTGTGCCAAAGCAGAATGCCTTTTAAACCTGGAGAAGCTGCTCCGGCAATTCCTTATCTCCAAGGA
  CACACTGTCGGTCCGGATGCTGGATGACACACGGGACCCCACTCCGCTCCTCAAGGAGATCCGGGATGAC
  AAGACAGCTACTATCATCATCCATGCCAACGCCTCCATGTCGCACACCATCCTCCTGAAGGCAGCTGAAC
  TGGGGATGGTGTCAGCCTATTACACCTACATCTTCACCAATCTGGAGTTCTCCCTCCAGAGAATGGACAG
  CCTTGTGGATGATAGAGTCAACATCTTAGGATTTTCCATTTTCAACCAATCCCATGCTTTCTTCCAAGAG
  TTCTCCCAGAGCCTCAACCAATCCTGGCAGGAGAACTGTGACCACGTGCCCTTCACCGGCCCTGCGTTGT
  CCTCAGCCCTACTGTTTGACGCTGTCTACGCTGTGGTGACAGCGGTACAGGAACTGAACCGGAGCCAGGA
  AATTGGCGTGAAGCCCCTGTCCTGTGGCTCGGCCCAGATCTGGCAGCATGGCACCAGCCTCATGAACTAC
  CTGCGCATGGTAGAATTGGAAGGTCTTACCGGCCACATTGAATTCAACAGCAAAGGCCAGAGGTCCAACT
  ATGCCTTGAAAATCTTACAGTTCACCAGGAATGGGTTTCGGCAGATTGGCCAGTGGCATGTGGCAGAGGG
  CCTCAGCATGGACAGCCGCCTCTACGCCTCCAACATCTCTGACAGTCTCTTCAACACCACCCTGGTCGTC
  ACCACCATCCTGGAAAACCCATACCTAATGCTGAAGGGGAACCACCAGGACATGGAAGGCAATGACCGGT
  ACGAGGGATTCTGCGTGGACATGCTCAAGGAGTTGGCTGAGATCCTACGGTTCAACTACAAGATCCGCCT
  CGTTGGGGATGGTGTTTACGGCGTACCTGAGGCCAACGGCACCTGGACGGGCATGGTTGGGGAGCTGATC
  GCTCGGAAAGCAGATCTGGCTGTGGCGGGCCTCACCATCACAGCTGAACGTGAGAAAGTGATTGATTTCT
  CCAAGCCGTTCATGACGCTGGGAATTAGTATTCTTTACAGAGTTCATATGGGACGCAGACCGGGCTATTT
  CTCCTTTCTGGACCCCTTTTCTCCAGGAGTCTGGCTCTTCATGCTTCTAGCTTATCTGGCTGTCAGCTGT
  GTCCTCTTCCTGGTGGCTCGGTTAACTCCTTATGAGTGGTACAGTCCACATCCTTGTGCACAGGGCCGGT
  GTAATCTCCTGGTCAACCAGTACTCTCTGGGCAACAGCCTCTGGTTTCCAGTGGGAGGCTTCATGCAGCA
  AGGCTCCACCATCGCCCCGCGGGCTTTGTCCACACGCTGCGTCAGTGGTGTCTGGTGGGCCTTCACGTTG
  ATCATCATCTCATCGTACACAGCCAACCTGGCAGCCTTCCTGACGGTGCAGCGCATGGAAGTGCCCATCG
  AGTCTGTGGATGACCTGGCTGACCAGACCGCCATTGAGTATGGCACGATTCACGGAGGCTCCAGCATGAC
  CTTCTTCCAAAATTCCCGCTACCAGACCTACCAGCGCATGTGGAATTACATGTACTCCAAGCAGCCCAGT
  GTGTTTGTGAAGAGCACAGAGGAGGGAATTGCCAGGGTGTTGAATTCCAACTACGCCTTCCTGCTGGAGT
  CCACCATGAACGAGTACTACCGGCAGAGGAACTGCAACCTCACTCAGATTGGGGGCCTGCTGGACACCAA
  GGGCTATGGGATTGGCATGCCAGTCGGCTCAGTGTTCCGGGATGAGTTCGACCTGGCCATTCTCCAGCTG
  CAGGAGAACAACCGCCTGGAGATTCTGAAGCGGAAGTGGTGGGAAGGGGGAAAATGTCCCAAGGAGGAGG
  ACCACAGAGCCAAAGGCCTGGGAATGGAGAATATTGGTGGAATCTTTGTGGTTCTTATTTGTGGCTTAAT
  AGTGGCCATTTTTATGGCTATGCTGGAGTTTTTATGGACTCTCAGACACTCAGAAGCATCAGAGGTGTCT
  GTGTGTCAGGAGATGATGACTGAGCTGCGCAGCATCATCCTGTGCCAGGACAATATCCATCCCCGGAGGC
  GGCGCTCTGGAGGCCTGCCACCCCAGCCCCCGGTCCTGGAGGAGCGCCGGCCCAGGGGTACAGCGACGCT
  CAGCAATGGCAAGCTATGTGGCGCCGGGGAGCCGGACCAACTGGCGCAGAGACTGGCTCAGGAGGCCGCC
  CTGGTGGCCCGAGGCTGCACGCACATCCGCGTGTGCCCGGAGTGCCGCCGCTTCCAGGGCCTGCGGGCTC
  GACCGTCGCCAGCGCGCAGCGAGGAGAGCCTGGAGTGGGACAAGACCACCAACAGCAGCGAGCCTGAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR208922 representing NM_012572
  Red=Cloning site Green=Tags(s)
  
  MPRVSAPLVLLPAWLLMVACSPHSLRIAAILDDPMECSRGERLSITLAKNRINRAPERLGKAKVEVDIFE
  LLRDSEYETAETMCQILPKGVVAVLGPSSSPASSSIISNICGEKEVPHFKVAPEEFVRFQLQRFTTLNLH
  PSNTDISVAVAGILNFFNCTTACLICAKAECLLNLEKLLRQFLISKDTLSVRMLDDTRDPTPLLKEIRDD
  KTATIIIHANASMSHTILLKAAELGMVSAYYTYIFTNLEFSLQRMDSLVDDRVNILGFSIFNQSHAFFQE
  FSQSLNQSWQENCDHVPFTGPALSSALLFDAVYAVVTAVQELNRSQEIGVKPLSCGSAQIWQHGTSLMNY
  LRMVELEGLTGHIEFNSKGQRSNYALKILQFTRNGFRQIGQWHVAEGLSMDSRLYASNISDSLFNTTLVV
  TTILENPYLMLKGNHQDMEGNDRYEGFCVDMLKELAEILRFNYKIRLVGDGVYGVPEANGTWTGMVGELI
  ARKADLAVAGLTITAEREKVIDFSKPFMTLGISILYRVHMGRRPGYFSFLDPFSPGVWLFMLLAYLAVSC
  VLFLVARLTPYEWYSPHPCAQGRCNLLVNQYSLGNSLWFPVGGFMQQGSTIAPRALSTRCVSGVWWAFTL
  IIISSYTANLAAFLTVQRMEVPIESVDDLADQTAIEYGTIHGGSSMTFFQNSRYQTYQRMWNYMYSKQPS
  VFVKSTEEGIARVLNSNYAFLLESTMNEYYRQRNCNLTQIGGLLDTKGYGIGMPVGSVFRDEFDLAILQL
  QENNRLEILKRKWWEGGKCPKEEDHRAKGLGMENIGGIFVVLICGLIVAIFMAMLEFLWTLRHSEASEVS
  VCQEMMTELRSIILCQDNIHPRRRRSGGLPPQPPVLEERRPRGTATLSNGKLCGAGEPDQLAQRLAQEAA
  LVARGCTHIRVCPECRRFQGLRARPSPARSEESLEWDKTTNSSEPE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_012572
• ORF Size: 2868 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_012572.1, NP_036704.1
• RefSeq Size: 3312 bp
• RefSeq ORF: 2871 bp
• Locus ID: 24406
• UniProt ID: Q01812
• Cytogenetics: 8q22
• MW: 107.3 kDa
• Gene Summary: kainate-preferring glutamate receptor subunit KA1, ligand-gated ion channel [RGD, Feb 2006]