C3 (NM_016994) Rat Tagged ORF Clone

CAT#: RR208499

C3 (Myc-DDK-tagged ORF) - Rat complement component 3 (C3), (10 ug)

Product Images

Circular map for RR208499

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: C3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR208499 representing NM_016994
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGACCCACGTCAGGGTCCCAGCTACTAGTGCTACTGCTGCTGTTGGCCAGCTCCCTGCTAGCTCTGG
  GGAGCCCCATGTACTCCATCATTACTCCCAATGTCCTGCGGCTGGAGAGTGAAGAGACTTTCATACTAGA
  GGCCCATGATGCTCAGGGTGATGTCCCAGTCACTGTCACTGTGCAAGACTTCCTAAAGAAGCAAGTGCTG
  ACCAGTGAGAAGACAGTGTTGACAGGAGCCACTGGACATCTGAACAGGGTCTCCATCAAGATTCCAGCCA
  GTAAGGAATTCAATGCAGATAAGGGGCACAAGTACGTGACAGTGGTGGCAAACTTCGGGGCAACAGTGGT
  GGAGAAAGCGGTGCTAGTAAGCTTTCAGAGTGGTTACCTCTTCATCCAGACAGACAAGACCATCTACACC
  CCAGGCTCCACTGTTTTCTATCGGATCTTCACTGTGGACAACAACCTATTGCCTGTGGGCAAGACAGTCG
  TCATCGTCATTGAGACCCCGGACGGCGTTCCCATCAAGAGAGACATTCTATCTTCCCACAACCAATATGG
  CATCTTGCCTTTGTCTTGGAACATTCCAGAACTGGTCAACATGGGGCAGTGGAAGATCCGAGCCTTCTAT
  GAACATGCACCAAAGCAGACCTTCTCTGCAGAGTTTGAGGTGAAGGAATACGTGCTGCCCAGTTTCGAAG
  TCCTGGTGGAGCCTACAGAGAAATTTTATTACATCGATGACCCAAAGGGCCTGGAAGTTTCCATCACAGC
  CAGATTCCTGTATGGGAAGAACGTGGACGGGACAGCTTTCGTGATCTTTGGGGTCCAGGATGAGGATAAG
  AAGATTTCTCTGGCCCAGTCCCTCACCCGCGTGCTGATCGAGGATGGTTCAGGGGAGGCAGTGCTCAGCC
  GAAAAGTGCTGATGGACGGGGTACGGCCCTCCAGCCCAGAAGCCCTAGTGGGGAAGTCCCTGTACGTCTC
  TGTCACTGTTATCCTGCACTCAGGTAGCGACATGGTAGAGGCAGAGCGCAGTGGGATCCCAATTGTCACT
  TCCCCGTACCAGATCCACTTCACCAAGACACCCAAATTCTTCAAGCCAGCCATGCCTTTCGACCTCATGG
  TGTTTGTGACCAACCCTGATGGCTCTCCAGCCCGCAGAGTGCCAGTAGTCACTCAGGGATCCGACGCGCA
  GGCTCTCACCCAGGATGATGGTGTGGCCAAGCTGAGCGTCAACACACCCAACAACCGCCAACCCCTGACT
  ATCACGGTCCGCACCAAGAAGGAGGGTATCCCGGACGCGCGGCAGGCCACCAGGACGATGCAGGCCCAGC
  CCTACAGCACTATGCACAATTCCAACAACTACCTGCACTTGTCAGTGTCTCGGGTGGAGCTCAAGCCTGG
  GGACAACCTCAATGTCAACTTCCACCTGCGCACGGACGCTGGCCAAGAGGCCAAGATCCGATACTACACC
  TATCTGGTTATGAACAAGGGGAAGTTACTGAAGGCAGGCCGTCAGGTTCGGGAGCCTGGCCAGGACCTGG
  TGGTCTTGTCACTGCCCATCACTCCAGAATTTATACCTTCCTTCCGCCTGGTGGCTTACTACACCCTGAT
  TGGAGCTAATGGCCAAAGGGAGGTGGTGGCCGACTCAGTGTGGGTGGATGTGAAGGACTCCTGTGTAGGC
  ACGCTGGTGGTGAAAGGTGACCCAAGAGATAACCGACAGCCCGCGCCTGGGCATCAAACGACACTAAGGA
  TCGAGGGGAACCAGGGGGCCCGAGTGGGGCTAGTGGCTGTGGACAAGGGGGTGTTTGTGCTGAACAAGAA
  GAACAAACTCACACAGAGCAAGATCTGGGATGTAGTAGAGAAGGCAGACATTGGCTGCACCCCAGGCAGT
  GGGAAGAACTATGCGGGTGTCTTCATGGATGCTGGCCTGACCTTCAAGACAAACCAAGGCCTGCAGACTG
  ATCAGAGAGAAGATCCTGAGTGCGCCAAGCCAGCTGCCCGCCGCCGTCGCTCAGTGCAGTTGATGGAAAG
  GAGGATGGACAAAGCTGGTCAGTACACCGACAAGGGTCTGCGGAAGTGTTGTGAGGATGGCATGCGTGAT
  ATCCCTATGAAGTACAGCTGCCAGCGCCGGGCTCGCCTCATCACCCAGGGCGAGAGCTGCCTGAAGGCCT
  TCATGGACTGCTGCAACTATATCACCAAGCTTCGTGAGCAGCACAGAAGAGACCATGTGCTGGGCCTGGC
  CAGGAGTGATGTGGATGAAGACATAATCCCAGAAGAAGATATTATCTCTAGAAGCCACTTCCCAGAGAGC
  TGGTTGTGGACCATAGAAGAGTTGAAAGAACCAGAGAAAAATGGAATCTCTACGAAGGTCATGAACATCT
  TTCTCAAAGATTCCATCACCACCTGGGAGATTCTGGCAGTGAGCTTGTCCGACAAGAAAGGGATCTGTGT
  GGCAGACCCCTATGAGATCACAGTGATGCAGGACTTCTTCATTGACCTGCGACTGCCCTACTCTGTGGTG
  CGCAATGAACAGGTGGAGATCAGAGCTGTGCTCTTCAATTACCGTGAACAGGAGAAACTTAAGGTAAGGG
  TGGAACTGTTGCATAACCCAGCCTTCTGCAGCATGGCCACTGCCAAGAAGCGGTACTACCAGACCATCGA
  AATCCCTCCCAAGTCCTCTGTGGCTGTGCCTTATGTCATTGTCCCCTTGAAGATCGGCCTCCAGGAGGTG
  GAGGTCAAGGCCGCCGTCTTCAACCACTTCATCAGTGATGGTGTCAAGAAGATACTGAAGGTCGTGCCAG
  AAGGAATGAGAGTCAACAAAACTGTGGCTGTCCGTACACTGGATCCAGAACACCTCGGTCAAGGGGGAGT
  GCAGAGGGAGGATGTACCTGCAGCAGACCTCAGTGACCAAGTGCCAGACACAGATTCTGAGACCAGAATT
  CTCCTGCAAGGGACCCCGGTGGCTCAGATGGCCGAGGACGCTGTGGACGGGGAGCGGCTGAAACACCTGA
  TCGTGACCCCCTCTGGCTGTGGGGAGCAGAACATGATTGGCATGACACCCACGGTCATTGCAGTACACTA
  TCTGGATCAGACCGAACAGTGGGAGAAATTCGGCCTAGAGAAGAGGCAAGAAGCTCTGGAGCTCATCAAG
  AAAGGGTACACCCAGCAGCTGGCTTTCAAACAGCCCAGCTCTGCCTATGCTGCCTTCAACAACCGGCCTC
  CCAGCACCTGGCTGACAGCCTATGTGGTCAAGGTCTTCTCTCTGGCTGCCAACCTCATCGCCATCGACTC
  TCAGGTCCTGTGTGGGGCTGTCAAATGGCTGATTCTGGAGAAACAGAAGCCAGATGGTGTCTTTCAGGAG
  GACGGACCAGTGATTCACCAAGAAATGATTGGTGGCTTCCGGAACACCAAGGAGGCAGATGTGTCGCTTA
  CAGCCTTTGTCCTCATCGCACTGCAGGAAGCCAGAGATATCTGTGAGGGGCAGGTCAACAGCCTTCCCGG
  GAGCATCAACAAGGCAGGGGAGTATCTTGAAGCCAGTTACCTGAACCTGCAGAGACCATACACAGTAGCC
  ATTGCTGGGTATGCCCTGGCCCTGATGAACAAACTGGAGGAACCTTACCTCACCAAGTTTCTGAACACAG
  CCAAAGATCGGAACCGCTGGGAGGAGCCTGGCCAGCAGCTCTACAATGTGGAGGCCACCTCCTACGCCCT
  CCTGGCCCTGCTGCTGCTGAAAGACTTTGACTCTGTGCCTCCTGTGGTGCGCTGGCTCAACGAGCAAAGA
  TACTACGGAGGTGGCTATGGCTCCACGCAGGCTACCTTCATGGTATTCCAAGCCTTGGCTCAATACCAAA
  CAGATGTCCCTGACCACAAGGACTTGAACATGGATGTGTCCCTCCACCTCCCCAGCCGCAGCTCCCCAAC
  TGTGTTTCGCCTGCTATGGGAAAGTGGCAGTCTCCTGAGATCAGAAGAGACCAAGCAGAATGAGGGCTTT
  TCTCTGACAGCCAAAGGAAAAGGCCAAGGCACACTGTCGGTGGTGACAGTGTATCACGCCAAAGTCAAAG
  GCAAAGCCACCTGCAAGAAGTTTGACCTCAGGGTCACCATAAAACCAGCCCCTGAGACAGCCAAGAAGCC
  CCAGGATGCCAAGAGTTCTATGATCCTTGACATCTGCACCAGGTACTTGGGAGACGTGGATGCTACTATG
  TCCATCCTGGACATCTCCATGATGACTGGCTTTATTCCAGACACAAACGACCTGGAACTGCTGAGCTCTG
  GAGTAGACAGATACATTTCCAAGTATGAGATGGACAAAGCCTTCTCCAACAAGAACACCCTCATCATCTA
  CCTAGAAAAGATCTCACACTCCGAAGAAGACTGCCTGTCCTTCAAAGTCCACCAGTTCTTTAACGTGGGA
  CTTATCCAGCCGGGGTCGGTCAAGGTCTACTCCTACTACAATCTAGAGGAGTCATGCACCCGGTTCTATC
  ATCCGGAGAAGGACGATGGAATGCTGAGCAAGCTGTGCCACAATGAAATGTGCCGCTGTGCAGAGGAGAA
  CTGCTTCATGCATCAGTCACAGGATCAGGTCAGCCTGAATGAACGACTAGACAAGGCTTGTGAGCCTGGA
  GTGGACTACGTGTACAAGACCAAGCTAACGACGATAGAGCTGTCGGATGATTTTGATGAGTACATCATGA
  CCATCGAGCAGGTCATCAAGTCAGGCTCAGATGAGGTGCAGGCAGGTCAGGAACGAAGGTTCATCAGCCA
  CGTCAAGTGCAGAAACGCCCTAAAGCTGCAGAAAGGGAAGCAGTACCTCATGTGGGGCCTCTCCTCCGAC
  CTCTGGGGAGAAAAGCCCAATACCAGCTACATCATTGGGAAGGACACGTGGGTGGAGCACTGGCCCGAGG
  CAGAGGAATGTCAGGATCAGAAGAACCAGAAACAGTGCGAAGACCTCGGGGCATTCACAGAAACAATGGT
  GGTTTTCGGCTGCCCCAAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR208499 representing NM_016994
  Red=Cloning site Green=Tags(s)
  
  MGPTSGSQLLVLLLLLASSLLALGSPMYSIITPNVLRLESEETFILEAHDAQGDVPVTVTVQDFLKKQVL
  TSEKTVLTGATGHLNRVSIKIPASKEFNADKGHKYVTVVANFGATVVEKAVLVSFQSGYLFIQTDKTIYT
  PGSTVFYRIFTVDNNLLPVGKTVVIVIETPDGVPIKRDILSSHNQYGILPLSWNIPELVNMGQWKIRAFY
  EHAPKQTFSAEFEVKEYVLPSFEVLVEPTEKFYYIDDPKGLEVSITARFLYGKNVDGTAFVIFGVQDEDK
  KISLAQSLTRVLIEDGSGEAVLSRKVLMDGVRPSSPEALVGKSLYVSVTVILHSGSDMVEAERSGIPIVT
  SPYQIHFTKTPKFFKPAMPFDLMVFVTNPDGSPARRVPVVTQGSDAQALTQDDGVAKLSVNTPNNRQPLT
  ITVRTKKEGIPDARQATRTMQAQPYSTMHNSNNYLHLSVSRVELKPGDNLNVNFHLRTDAGQEAKIRYYT
  YLVMNKGKLLKAGRQVREPGQDLVVLSLPITPEFIPSFRLVAYYTLIGANGQREVVADSVWVDVKDSCVG
  TLVVKGDPRDNRQPAPGHQTTLRIEGNQGARVGLVAVDKGVFVLNKKNKLTQSKIWDVVEKADIGCTPGS
  GKNYAGVFMDAGLTFKTNQGLQTDQREDPECAKPAARRRRSVQLMERRMDKAGQYTDKGLRKCCEDGMRD
  IPMKYSCQRRARLITQGESCLKAFMDCCNYITKLREQHRRDHVLGLARSDVDEDIIPEEDIISRSHFPES
  WLWTIEELKEPEKNGISTKVMNIFLKDSITTWEILAVSLSDKKGICVADPYEITVMQDFFIDLRLPYSVV
  RNEQVEIRAVLFNYREQEKLKVRVELLHNPAFCSMATAKKRYYQTIEIPPKSSVAVPYVIVPLKIGLQEV
  EVKAAVFNHFISDGVKKILKVVPEGMRVNKTVAVRTLDPEHLGQGGVQREDVPAADLSDQVPDTDSETRI
  LLQGTPVAQMAEDAVDGERLKHLIVTPSGCGEQNMIGMTPTVIAVHYLDQTEQWEKFGLEKRQEALELIK
  KGYTQQLAFKQPSSAYAAFNNRPPSTWLTAYVVKVFSLAANLIAIDSQVLCGAVKWLILEKQKPDGVFQE
  DGPVIHQEMIGGFRNTKEADVSLTAFVLIALQEARDICEGQVNSLPGSINKAGEYLEASYLNLQRPYTVA
  IAGYALALMNKLEEPYLTKFLNTAKDRNRWEEPGQQLYNVEATSYALLALLLLKDFDSVPPVVRWLNEQR
  YYGGGYGSTQATFMVFQALAQYQTDVPDHKDLNMDVSLHLPSRSSPTVFRLLWESGSLLRSEETKQNEGF
  SLTAKGKGQGTLSVVTVYHAKVKGKATCKKFDLRVTIKPAPETAKKPQDAKSSMILDICTRYLGDVDATM
  SILDISMMTGFIPDTNDLELLSSGVDRYISKYEMDKAFSNKNTLIIYLEKISHSEEDCLSFKVHQFFNVG
  LIQPGSVKVYSYYNLEESCTRFYHPEKDDGMLSKLCHNEMCRCAEENCFMHQSQDQVSLNERLDKACEPG
  VDYVYKTKLTTIELSDDFDEYIMTIEQVIKSGSDEVQAGQERRFISHVKCRNALKLQKGKQYLMWGLSSD
  LWGEKPNTSYIIGKDTWVEHWPEAEECQDQKNQKQCEDLGAFTETMVVFGCPN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_016994
• ORF Size: 4989 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_016994.2, NP_058690.2
• RefSeq Size: 5091 bp
• RefSeq ORF: 4992 bp
• Locus ID: 24232
• UniProt ID: P01026
• Cytogenetics: 9q12
• MW: 186.3 kDa
• Gene Summary: putative complement component C3; likely involved in innate immune response [RGD, Feb 2006]