Klc3 (NM_138520) Rat Tagged ORF Clone

CAT#: RR208035

Klc3 (Myc-DDK-tagged ORF) - Rat kinesin light chain 3 (Klc3), (10 ug)

Product Images

Circular map for RR208035

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Klc3
• Synonyms: Klct
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR208035 representing NM_138520
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTGTGCAGGTGGCAGCCCCTGGAAGCACAGGGCTGGGCCCAGAACGCCTGAACCCGGAGGAGCTGG
  TGCGGCAGACGCGACAGGTTGTACAGGGACTGGAGGCCCTGCGGGCCGAGCACCATAGTCTGGCTGGGCA
  CTTGGCGGAGGCCCTGGCTGGACCAGGCCCTGTAGCGGGTGTAGAACTGCTGGAAGAGAAGCAACAGGTG
  GTGAACCACTCCCTGGAGGCCATTGAGCTGGGACTGGGTGAGGCCCAGGTGCTGCTGGCCCTGTCTGCAC
  ACGTGGGTGTGTTGGAGGCTGAGAAGCAGAGGCTACGGGCACAGGCACGGAGGCTAGCCCAAGAGAACAC
  ATGGCTTCGAGAGGAGCTGGAGGAGACACAGAGGCGACTGCGGGCCAGTGAGGAGGCTGTGGCCCAGCTG
  GAAGAGGAGAAAAGCCACCTTCAGTTCCTGGGGCAGCTGAGGCAGTATGACCCGCCCGAGGAGAGCCAGA
  GGCCGGATTCCCCGCCTCGCCGAGACAGCCTGGCTTCTCTCTTCCCCAGTGAAGAGGAGGAAAAGAAAGG
  TCCTGAGGCAGCAGGAGCAGCAGCAGCGCAGCAGGGTGGCTATGAGATCCCAGATCGTCTACGGACTCTG
  CACAACGTGGTGATCCAGTATGCTAGCCACGTCCGCTATGAGGTGGCAATTCCGCTGTGTCGCCAGGCAT
  TGGAGGACCTGGTGCGGAGCTCAGGCCACTTCCACCCTGACGTACCCACCATGGTCGACTTCTTGGCACT
  TGTGTACAGGGACCAGAACAAGTACAAGGAGGCCACGGAGTTTCTTCACGACGCCCTGCAGATCCGGGAA
  CAGACGCTGGGTCCTGAACACCCTGCAGTGGCCGCCACCCTCAACAACCTGGCAGTCCTTTACGGAAAAC
  GTGGCCGTTACCGGGAGGCAGAACCCCTCTGCCAGCGTGCTCTGGAGATCCGTGAGAAGGTCCTGGGTGC
  TGACCACCCTGATGTGGCCAAGCAGCTCAACAACCTGGCCCTGCTGTGCCAGAACCAGGGCAAGTTTCAG
  GATGTGGAGCGGCACTATGCAAGGGCCCTGAGTATCTACGAGGCCCTGGGGGGACCACAAGACCCAAATG
  TGGCTAAGATCAAGAACAACCTGGCCTCGGCCTACCTGAAGCAGAACAAGTATCAGCAGGCAGAGGAGCT
  ATACAAGGAGATCCTCAGCCAGGAGGCCCTGCCTGCCCCGCTTGGAGCCCCCCAGGGAGGCACAGCAGGT
  GAAGCACAACAGCAGGTCCTACGCAGGAGCAGTTCCTTCTCTAAGCTCAGAGAGTCCATCCGGCGTGGAA
  GTGAGAAGCTGGTCTCCCGCCTCCGAGGGGAGAGCATGGCAGGGGCTGCAGGGATGAAGAGAGCCATGTC
  ACTCAATATGCTGAACGTCGATGGTCCAAGGGCTGCCAGGATGCAGCTCTCAACCCAGCACCTGAATGAG
  GCCTCTCGGACCCTCAGCGCCAGCACGCAGGATTTGAGCCCGCGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR208035 representing NM_138520
  Red=Cloning site Green=Tags(s)
  
  MSVQVAAPGSTGLGPERLNPEELVRQTRQVVQGLEALRAEHHSLAGHLAEALAGPGPVAGVELLEEKQQV
  VNHSLEAIELGLGEAQVLLALSAHVGVLEAEKQRLRAQARRLAQENTWLREELEETQRRLRASEEAVAQL
  EEEKSHLQFLGQLRQYDPPEESQRPDSPPRRDSLASLFPSEEEEKKGPEAAGAAAAQQGGYEIPDRLRTL
  HNVVIQYASHVRYEVAIPLCRQALEDLVRSSGHFHPDVPTMVDFLALVYRDQNKYKEATEFLHDALQIRE
  QTLGPEHPAVAATLNNLAVLYGKRGRYREAEPLCQRALEIREKVLGADHPDVAKQLNNLALLCQNQGKFQ
  DVERHYARALSIYEALGGPQDPNVAKIKNNLASAYLKQNKYQQAEELYKEILSQEALPAPLGAPQGGTAG
  EAQQQVLRRSSSFSKLRESIRRGSEKLVSRLRGESMAGAAGMKRAMSLNMLNVDGPRAARMQLSTQHLNE
  ASRTLSASTQDLSPR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_138520
• ORF Size: 1515 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_138520.1, NP_612529.1
• RefSeq Size: 1697 bp
• RefSeq ORF: 1518 bp
• Locus ID: 171549
• UniProt ID: Q68G30
• Cytogenetics: 1q21
• MW: 55.9 kDa
• Gene Summary: light chain of kinesin; may be involved in cargo binding and regulation of kinesin activity [RGD, Feb 2006]