Gucy2c (NM_013170) Rat Tagged ORF Clone

CAT#: RR204036

Gucy2c (Myc-DDK-tagged ORF) - Rat guanylate cyclase 2C (Gucy2c), (10 ug)

Product Images

Circular map for RR204036

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Gucy2c
• Synonyms: GCC
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR204036 representing NM_013170
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACGTCACTCCTGGGCTTGGCTGTGCGGTTACTGCTCTTCCAACCCACGCTGATGTTCTGGGCCTCCC
  AGGTGAGGCAGAAGTGCCACAATGGCACCTACGAGATCAGTGTCCTGATGATGGATAACTCAGCCTACAA
  AGAACCTTTGCAAAACTTGAGGGATGCTGTGGAGGAAGGACTGGACATCGTGCGAAAGCGCCTGCGCGAA
  GCCGAACTAAATGTGACTGTGAACGCAACCTTCATCTACTCCGATGGTCTGATCCATAAGTCAGGTGACT
  GCCGGAGCAGTACCTGTGAAGGCCTTGACCTCCTCAGGGAAATTACAAGAGATCGTAAGATGGGCTGTGT
  CCTCATGGGGCCCTCGTGCACGTATTCCACCTTCCAGATGTACCTTGACACAGAGTTGAACTATCCCATG
  ATTTCCGCTGGAAGTTTTGGATTGTCCTGTGACTATAAGGAAACCCTAACCAGGATCCTGCCTCCAGCCA
  GGAAGCTGATGTACTTCTTGGTCGATTTCTGGAAAGTCAACAATGCACCTTTCAAAACCTTTTCCTGGAA
  CTCTTCATATGTTTACAAGAACGGATCGGAGCCTGAAGATTGTTTCTGGTACCTCAACGCTCTAGAGGCT
  GGGGTGTCCTATTTTTCTGAGGTGCTCAGCTTCAAGGATGTATTGAGACGCAGTGAACAGTTCCAGGAAA
  TCCTAATGGGCCGTAACAGGAAGAGCAATGTGATTGTTATGTGTGGCACGCCAGAAACCTTCTACAATGT
  GAAAGGTGACCTCAAAGTGGCTGACGACACTGTTGTCATCCTGGTAGATCTGTTCAGTAACCATTACTTT
  GAGGATGACACCAGAGCTCCTGAGTATATGGACAATGTCCTCGTCCTGACACTGCCTCCTGAAAAGTTCA
  TCGCGAACGCCTCTGTCTCTGGGAGGTTTCCATCGGAAAGAAGCGACTTTTCTCTCGCTTACTTGGAGGG
  GACCTTGCTGTTTGGACACATGCTGCAGACGTTTCTTGAAAATGGAGAATCTGTCACCACGCCCAAGTTC
  GCTCGTGCGTTCAGGAATCTCACTTTTCAAGGCTTAGAGGGGCCCGTGACTCTGGATGACAGTGGGGACA
  TTGACAACATTATGTGTCTTCTGTATGTGTCTCTGGATACCAGGAAATACAAGGTTCTTATGGCGTATGA
  CACCCATAAAAACCAAACGATCCCTGTGGCTACGAGCCCCAACTTCATCTGGAAGAACCACAGACTCCCT
  AATGACGTTCCTGGGCTGGGCCCTCAAATCCTGATGATTGCCGTCTTCACGCTCACGGGGATTGTGGTCG
  TTCTGCTGCTGATTGCCCTCCTTGTGCTCAGAAAATACAGAAGAGATCATGAACTTCGACAGAAGAAATG
  GTCCCACATCCCTTCTGAAAATATCTTTCCTCTGGAGACCAACGAGACCAACCATGTCAGCCTGAAGATT
  GACGATGACAGGAGGCGGGATACAATCCAGAGAGTGCGACAGTGCAAATACGACAAGAAGAAAGTGATCC
  TGAAAGACCTCAAGCACTGTGATGGTAACTTCAGTGAGAAGCAGAAGATAGAACTGAACAAGCTACTGCA
  GTCAGACTACTACAACCTGACCAAGTTCTACGGCACCGTGAAGCTAGACACCAGGATCTTTGGGGTGGTC
  GAGTACTGCGAGAGGGGGTCCCTCCGGGAAGTGTTAAATGACACGATTTCCTACCCTGATGGCACGTTCA
  TGGATTGGGAGTTTAAGATCTCTGTCTTAAATGACATTGCTAAGGGGATGTCCTATCTGCACTCCAGTAA
  GATTGAAGTCCACGGGCGTCTGAAGTCCACCAACTGCGTGGTGGACAGTCGCATGGTGGTGAAGATCACT
  GATTTTGGGTGCAATTCCATCCTGCCTCCAAAGAAAGACCTGTGGACTGCCCCCGAGCACCTGCGCCAAG
  CTACTATCTCTCAGAAAGGAGACGTGTACAGCTTCAGCATCATTGCCCAGGAGATCATCCTCCGCAAGGA
  AACTTTCTACACGCTGAGCTGCCGGGATCAGAATGAGAAGATTTTCAGAGTGGAAAATTCCTATGGGACG
  AAACCCTTCCGCCCAGATCTCTTCCTGGAAACCGCAGATGAGAAGGAGCTGGAGGTCTATCTATTGGTCA
  AAAGCTGTTGGGAGGAGGATCCAGAAAAGAGACCAGATTTCAAGAAAATCGAGAGCACACTAGCCAAGAT
  ATTTGGCCTTTTTCATGACCAAAAAAATGAATCTTACATGGACACCTTGATCCGACGTCTACAGCTGTAT
  TCTCGGAACCTGGAGCACCTGGTGGAGGAAAGGACTCAGCTGTACAAGGCCGAGAGGGACAGGGCTGACC
  ACCTTAACTTTATGCTGCTCCCACGGCTGGTGGTAAAGTCCCTGAAGGAGAAAGGCATCGTGGAGCCAGA
  GCTGTACGAAGAAGTCACAATCTATTTCAGTGACATTGTCGGTTTCACGACCATCTGCAAGTACAGCACG
  CCCATGGAGGTGGTGGACATGCTGAATGACATCTACAAGAGTTTTGACCAGATTGTGGATCACCACGACG
  TCTACAAGGTAGAAACCATCGGCGATGCCTACGTGGTGGCCAGCGGCCTGCCTATGAGAAACGGCAACCG
  GCATGCAGTGGACATTTCCAAGATGGCCTTGGACATCCTCAGCTTCATGGGGACCTTTGAGCTGGAGCAT
  CTCCCCGGCCTCCCCGTGTGGATTCGCATTGGGGTTCATTCTGGCCCCTGTGCTGCTGGTGTGGTGGGGA
  TCAAGATGCCTCGTTATTGCCTGTTTGGAGACACTGTCAACACTGCCTCCAGGATGGAGTCCACCGGCCT
  TCCCTTAAGGATTCACATGAGCAGCTCCACCATTGCCATCCTGAGGAGAACGGATTGCCAGTTCCTGTAC
  GAAGTGAGGGGAGAAACGTACTTAAAGGGAAGAGGGACCGAGACCACATACTGGCTGACTGGGATGAAGG
  ACCAAGAGTACAACCTGCCAACCCCACCAACAGTGGAGAACCAACAGCGTCTGCAAACTGAGTTCTCAGA
  CATGATCGTTAGTGCCTTACAGAAAAGACAGGCCTCGGGCGTGAAGAGCCGGAGGCCCACTCGGGTGGCC
  AGCTACAAGAAAGGCTTTTTGGAATACATGCAGCTGAACAACTCAGACCACGATAGCACCTATTTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR204036 representing NM_013170
  Red=Cloning site Green=Tags(s)
  
  MTSLLGLAVRLLLFQPTLMFWASQVRQKCHNGTYEISVLMMDNSAYKEPLQNLRDAVEEGLDIVRKRLRE
  AELNVTVNATFIYSDGLIHKSGDCRSSTCEGLDLLREITRDRKMGCVLMGPSCTYSTFQMYLDTELNYPM
  ISAGSFGLSCDYKETLTRILPPARKLMYFLVDFWKVNNAPFKTFSWNSSYVYKNGSEPEDCFWYLNALEA
  GVSYFSEVLSFKDVLRRSEQFQEILMGRNRKSNVIVMCGTPETFYNVKGDLKVADDTVVILVDLFSNHYF
  EDDTRAPEYMDNVLVLTLPPEKFIANASVSGRFPSERSDFSLAYLEGTLLFGHMLQTFLENGESVTTPKF
  ARAFRNLTFQGLEGPVTLDDSGDIDNIMCLLYVSLDTRKYKVLMAYDTHKNQTIPVATSPNFIWKNHRLP
  NDVPGLGPQILMIAVFTLTGIVVVLLLIALLVLRKYRRDHELRQKKWSHIPSENIFPLETNETNHVSLKI
  DDDRRRDTIQRVRQCKYDKKKVILKDLKHCDGNFSEKQKIELNKLLQSDYYNLTKFYGTVKLDTRIFGVV
  EYCERGSLREVLNDTISYPDGTFMDWEFKISVLNDIAKGMSYLHSSKIEVHGRLKSTNCVVDSRMVVKIT
  DFGCNSILPPKKDLWTAPEHLRQATISQKGDVYSFSIIAQEIILRKETFYTLSCRDQNEKIFRVENSYGT
  KPFRPDLFLETADEKELEVYLLVKSCWEEDPEKRPDFKKIESTLAKIFGLFHDQKNESYMDTLIRRLQLY
  SRNLEHLVEERTQLYKAERDRADHLNFMLLPRLVVKSLKEKGIVEPELYEEVTIYFSDIVGFTTICKYST
  PMEVVDMLNDIYKSFDQIVDHHDVYKVETIGDAYVVASGLPMRNGNRHAVDISKMALDILSFMGTFELEH
  LPGLPVWIRIGVHSGPCAAGVVGIKMPRYCLFGDTVNTASRMESTGLPLRIHMSSSTIAILRRTDCQFLY
  EVRGETYLKGRGTETTYWLTGMKDQEYNLPTPPTVENQQRLQTEFSDMIVSALQKRQASGVKSRRPTRVA
  SYKKGFLEYMQLNNSDHDSTYF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_013170
• ORF Size: 3216 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_013170.1, NP_037302.1
• RefSeq Size: 3775 bp
• RefSeq ORF: 3219 bp
• Locus ID: 25711
• UniProt ID: P23897
• Cytogenetics: 4q43
• MW: 123.5 kDa
• Gene Summary: guanylyl cyclase; functions as receptor for heat-stable enterotoxin responsible for acute diarrhea [RGD, Feb 2006]