Taf7 (NM_001107394) Rat Tagged ORF Clone

CAT#: RR202402

Taf7 (Myc-DDK-tagged ORF) - Rat TAF7 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 55kDa (Taf7), (10 ug)

Product Images

Circular map for RR202402

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Taf7
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR202402 representing NM_001107394
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTAAGAGCAAAGATGATGCACCTCATGAGCTAGAGAGCCAGTTTATCTTACGGTTGCCCCCAGAAT
  ATGCCGCTACTGTGAGGAGGGCAGTGCAGTCTGGGCACGTCAACCTGAAAGATAGACTGAGCATTGAGTT
  ACACCCTGATGGGCGTCATGGGATTGTCAGAGTGGATAGAGTGCCCCTGGCTGCAAAATTGGTAGATTTG
  CCGTGTGTTACGGAGAGCTTGAAAACCATTGATAAAAAAACCTTTTACAAGACAGCTGATATCTCTCAGA
  TGCTTGTAGCCACAGTAGATGGTGATCTATACCCTCCTGTGGAGGAGCCAGCTGCTACTGCTGATCCCAA
  AGCAAACAAGAAAAAAGATAAGGACAAAGAGAAAAAATTTGTTTGGAACCATGGAATTACTCTACCTCTA
  AAAAATGTCAGAAAGAGAAGGTTCCGGAAGACAGCAAAGAAGAAGTACATTGAATCTCCAGATGTGGAAA
  AAGAAGTAAAGCGGCTGCTGAGTACAGATGCAGAAGCTGTCAGTACTCGTTGGGAGATAATTGCTGAAGA
  TGAAACAAAGGAGGCAGAAAATCAAGGCCTTGATATCTCCTCTCCTGGAATGTCTGGCCACAGGCAGGGC
  CATGACTCACTAGAGCATGATGAGCTTCGAGAGATATTCAATGACCTCAGCAGCAGCAGCGAAGATGAAG
  AAGATGTAAACATCATTGACACTGAAGAGGATCTGGAGAGACAGCTACAGGACAAGCTAAATGAGTCAGA
  TGAACAACACCAAGAAAATGAAGGAACCAATCAGTTGGTTATGGGGATTCAGAAGCAGATTGATAATATG
  AAAGGCAAGCTTCAAGAAACCCAAGACAGAGCAAAGCGCCAGGAGGATCTCATCATGAAGGTAGAAAACC
  TGGCTCTGAAGAACAGGTTTCAAGCTGTTCTGGATGAGCTCAAACAGAAGGAAGACCGGGAAAAGGAGCA
  GCTTAGCTCTTTGCAAGAAGAGCTGGAATCACTTCTGGAGAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR202402 representing NM_001107394
  Red=Cloning site Green=Tags(s)
  
  MSKSKDDAPHELESQFILRLPPEYAATVRRAVQSGHVNLKDRLSIELHPDGRHGIVRVDRVPLAAKLVDL
  PCVTESLKTIDKKTFYKTADISQMLVATVDGDLYPPVEEPAATADPKANKKKDKDKEKKFVWNHGITLPL
  KNVRKRRFRKTAKKKYIESPDVEKEVKRLLSTDAEAVSTRWEIIAEDETKEAENQGLDISSPGMSGHRQG
  HDSLEHDELREIFNDLSSSSEDEEDVNIIDTEEDLERQLQDKLNESDEQHQENEGTNQLVMGIQKQIDNM
  KGKLQETQDRAKRQEDLIMKVENLALKNRFQAVLDELKQKEDREKEQLSSLQEELESLLEK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001107394
• ORF Size: 1023 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001107394.2, NP_001100864.1
• RefSeq Size: 1796 bp
• RefSeq ORF: 1026 bp
• Locus ID: 307485
• Cytogenetics: 18p11
• MW: 39.1 kDa