Kiss1r (NM_023992) Rat Untagged Clone

CAT#: RN214267

Kiss1r (untagged ORF) - Rat KISS1 receptor (Kiss1r), (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Kiss1r
• Synonyms: Gpr54
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN214267 representing NM_023992
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGCAGAGGCGACGTTGGGTCCGAACGTGAGCTGGTGGGCTCCGTCCAACGCTTCGGGATGCCCGG
  GCTGCGGTGTCAATGCCTCGGATGGCCCAGGCTCCGCGCCAAGGCCCCTGGATGCCTGGCTGGTGCCCCT
  GTTTTTCGCTGCCCTAATGTTGCTGGGGCTAGTCGGGAACTCACTGGTCATCTTCGTTATCTGCCGCCAC
  AAGCACATGCAGACCGTCACCAATTTCTACATCGCTAACCTGGCGGCCACAGATGTCACTTTCCTTCTGT
  GCTGCGTACCCTTCACCGCGCTCCTCTATCCGCTGCCCACCTGGGTGCTGGGAGACTTCATGTGCAAATT
  CGTCAACTACATCCAGCAGGTCTCGGTGCAAGCCACATGTGCCACTTTGACAGCCATGAGTGTGGACCGC
  TGGTACGTGACTGTGTTCCCGCTGCGTGCACTTCACCGCCGCACTCCGCGCCTGGCCCTGACTGTCAGCC
  TTAGCATCTGGGTGGGTTCCGCAGCTGTTTCCGCCCCGGTGCTGGCTCTGCACCGCCTGTCGCCCGGGCC
  TCACACCTACTGCAGTGAGGCGTTTCCCAGCCGTGCCCTGGAGCGCGCTTTCGCGCTCTACAACCTGCTG
  GCCCTATACCTGCTGCCGCTGCTCGCCACCTGCGCCTGCTACGGTGCCATGCTGCGCCACCTGGGCCGCG
  CCGCTGTACGCCCCGCACCCACTGATGGCGCCCTGCAGGGGCAGCTGCTAGCACAGCGCGCTGGAGCAGT
  GCGCACCAAGGTCTCCCGGCTGGTGGCCGCTGTCGTCCTGCTCTTCGCCGCCTGCTGGGGCCCGATCCAG
  CTGTTCCTGGTGCTTCAAGCCCTGCCGCTCGGGGGCCTGGCACCCTCGAAGCTATGCGCCTACGCGCTCA
  AGATCTGGGCTCACTGCATGTCCTACAGCAATTCTGCGCTCAACCCGCTGCTCTATGCCTTCCTGGGTTC
  CCACTTCAGACAGGCCTTCTGCCGCGTGTGCCCCTGCGGCCCGCAACGCCAGCGTCGGCCCCACGCGTCA
  GCGCACTCGGACCGAGCCGCACCCCATAGTGTGCCGCACAGCCGGGCTGCGCACCCTGTCCGGGTCAGGA
  CCCCCGAGCCTGGGAACCCTGTGGTGCACTCGCCCTCTGTTCAGGATGAACACACTGCCCCACTCTGA
  
  ACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGA
  TTACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-NotI
• ACCN: NM_023992
• Insert Size: 1188 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_023992.1, NP_076482.1
• RefSeq Size: 1502 bp
• RefSeq ORF: 1191 bp
• Locus ID: 78976
• UniProt ID: Q924U1
• Cytogenetics: 7q11
• Gene Summary: a G protein-coupled receptor; human homolog may bind ligand and metastasis suppressor gene KiSS-1 [RGD, Feb 2006]
  Transcript Variant: This variant (1) encodes the longer isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.