Pycr3 (NM_001011993) Rat Untagged Clone

CAT#: RN212963

Pycrl (untagged ORF) - Rat pyrroline-5-carboxylate reductase-like (Pycrl), (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Pycr3
• Synonyms: Pycrl
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN212963 representing NM_001011993
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGATGAGATGTCTGAACCTCGGCGTGTGGGCTTCGTAGGTGCTGGTCGCATGGCGGAAGCCATTG
  CCCAAGGCCTCATCCGAGCAGGCAAAGTAGAAGCAAAGCAAGTGCTGGCCAGTGCACCAACAGACAAGAA
  CCTCTGCCACTTCAGGGCTCTGGGTTGCCAGACTACTCACTCCAACCACGAGGTGCTGCAAAACTGCCCA
  CTCGTCATCTTTGCCACCAAACCCCAAGTCCTGCCAGCTGTTCTGGCAGAAGTGGCCCCTGTAGTCACCA
  CTGAGCACATCATTGTATCCGTGGCTGCTGGGATCTCTCTGAGCTCTATGGAGGAGCTGTTACCCCCGAA
  AACACGAGTACTGCGAGTCTCTCCCAATCTACCCTGCGTAGTTCAGGAGGGAGCTATGGTTATGACACGG
  GGCCACCATGCTGGGAATGAAGACGCCAAGCTCCTACAGAACTTGCTGGAAGCCTGTGGGCAGTGCATAG
  AGGTTCCTGAGTCCTACGTCGATATCCACACTGGCCTCAGCGGCAGTGGTGTGGCCTTTGTGTGTACATT
  TTCGGAGGCCCTGGCTGAAGGCGCCATCAAGATGGGCATGCCCAGTGACCTGGCCCACCGCATTGCCGCT
  CAGACCCTGCTGGGGACAGCCAAAATGCTGCAGCAGGAAGGGAAGCACCCAGCCCAGCTGCGGACGGATG
  TGCTCACACCAGCTGGAACCACCATCCACGGGCTTCATGCCCTAGAGCAGGGCGGTTTTCGAGCAGCTGC
  CATGAGTGCTGTGGAAGCAGCTACCTGCCGGGCTAAAGAGCTCAGCAAGAAGTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001011993
• Insert Size: 825 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001011993.1, NP_001011993.1
• RefSeq Size: 1489 bp
• RefSeq ORF: 825 bp
• Locus ID: 300035
• UniProt ID: Q5PQJ6
• Cytogenetics: 7q34
• Gene Summary: Enzyme that catalyzes the last step in proline biosynthesis. Proline is synthesized from either glutamate or ornithine; both are converted to pyrroline-5-carboxylate (P5C), and then to proline via pyrroline-5-carboxylate reductases (PYCRs). PYCRL is exclusively linked to the conversion of ornithine to proline.[UniProtKB/Swiss-Prot Function]