SFRS12 (SREK1) (NM_001270493) Human Tagged ORF Clone

CAT#: RG235595

SREK1 (tGFP-tagged) - Human splicing regulatory glutamine/lysine-rich protein 1 (SREK1), transcript variant 4

Product Images

Circular map for RG235595

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: SFRS12
• Synonyms: SFRS12; SRrp86; SRrp508
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG235595 representing NM_001270493.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGAACAGCGGCGGCGGCTTCGGTTTGGGCTTAGGCTTCGGCCTCACCCCCACGTCGGTGATTCAGGTG
  ACGAATCTGTCGTCGGCGGTGACCAGCGAGCAGATGCGGACGCTTTTTTCCTTCCTAGGAGAAATCGAG
  GAGCTGCGGCTCTACCCCCCGGACAACGCACCTCTTGCTTTTTCCTCCAAAGTATGTTATGTTAAGTTT
  CGTGATCCATCAAGTGTTGGCGTGGCCCAGCATCTAACTAACACGGTTTTTATTGACAGAGCTCTGATA
  GTTGTTCCTTGTGCAGAAGATGAC
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC
  
  Protein Sequence

  >Peptide sequence encoded by RG235595
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MNSGGGFGLGLGFGLTPTSVIQVTNLSSAVTSEQMRTLFSFLGEIEELRLYPPDNAPLAFSSKVCYVKF
  RDPSSVGVAQHLTNTVFIDRALIVVPCAEDD
  TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV
  MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED
  SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA
  FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001270493
• ORF Size: 300 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).

Reference Data

• RefSeq: NM_001270493.1, NP_001257422.1
• RefSeq Size: 2143 bp
• RefSeq ORF: 303 bp
• Locus ID: 140890
• Cytogenetics: 5q12.3
• MW: 11.2 kDa
• Gene Summary: This gene encodes a member of a family of serine/arginine-rich (SR) splicing proteins containing RNA recognition motif (RRM) domains. The encoded protein interacts with other SR proteins to modulate splice site selection. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jul 2012]