GPR172B (SLC52A1) (NM_001104577) Human Tagged ORF Clone

CAT#: RG225737

SLC52A1 (tGFP-tagged) - Human G protein-coupled receptor 172B (GPR172B), transcript variant 1

Product Images

Circular map for RG225737

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: GPR172B
• Synonyms: GPCR42; GPR172B; hRFT1; huPAR-2; PAR2; RBFVD; RFT1; RFVT1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG225737 representing NM_001104577
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGCACCCACGCTGGGCCGTCTGGTGCTGACCCACCTGCTGGTGGCCCTTTTTGGCATGGGCTCCT
  GGGCTGCTGTGAACGGGATCTGGGTGGAGCTGCCTGTGGTGGTAAAAGACCTTCCAGAGGGTTGGAGCCT
  CCCCTCATACCTCTCTGTGGTTGTGGCGCTGGGAAACCTGGGTCTGCTGGTGGTGACCCTGTGGAGGCAG
  CTGGCCCCGGGCAAGGGCGAGCAGGTCCCCATCCAGGTGGTACAGGTGCTGAGTGTAGTGGGCACAGCCC
  TGCTGGCCCCTCTGTGGCACCACGTGGCCCCAGTGGCAGGGCAGCTCCACTCTGTGGCCTTCCTAACTCT
  GGCCTTGGTGTTGGCAATGGCCTGTTGTACCTCTAATGTCACTTTCCTGCCCTTCCTGAGCCACCTGCCA
  CCTCCTTTCTTACGGTCTTTCTTCCTGGGTCAGGGTCTCAGTGCCCTACTCCCCTGTGTGCTGGCCCTAG
  TGCAAGGTGTGGGCCGCCTCGAGTGCCCACCAGCGCCCACCAATGGCACCTCTGGGCCTCCCCTCGACTT
  CCCTGAGCGTTTTCCTGCCAGCACCTTCTTCTGGGCACTGACTGCCCTTCTGGTCACTTCAGCTGCCGCC
  TTCCGGGGTCTCCTGTTGCTGTTGCCATCACTACCCTCTGTAACCACAGGGGGCTCAGGGCCTGAACTTC
  AACTGGGATCCCCAGGAGCAGAGGAGGAAGAGAAGGAGGAAGAAGAGGCTTTGCCATTGCAGGAGCCACC
  GAGCCAGGCAGCAGGCACCATCCCTGGCCCAGACCCTGAGGCCCATCAGCTGTTCTCAGCCCATGGTGCC
  TTCCTGCTGGGCCTGATGGCCTTCACCAGTGCCGTGACCAATGGCGTGCTGCCTTCTGTGCAGAGCTTTT
  CCTGTTTGCCCTATGGGCGCCTGGCCTACCACCTGGCTGTGGTGCTGGGCAGTGCCGCCAACCCCCTTGC
  CTGCTTCCTGGCCATGGGCGTGCTGTGCAGGTCCCTGGCAGGGCTGGTTGGTCTTTCTCTGCTGGGCATG
  CTCTTTGGGGCCTACCTGATGGCACTGGCAATCCTGAGCCCCTGCCCACCCCTGGTGGGCACCACTGCAG
  GGGTGGTCCTTGTGGTGCTGTCGTGGGTGCTGTGTCTGTGTGTGTTCTCATATGTGAAGGTGGCTGCAAG
  CTCCCTGCTGCATGGTGGGGGTCGGCCGGCATTGCTGGCAGCTGGTGTGGCCATCCAAGTGGGCTCCCTG
  CTTGGTGCCGGTGCCATGTTCCCTCCCACCAGCATCTACCACGTGTTTCAAAGCAGAAAGGACTGTGTAG
  ACCCCTGTGGCCCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG225737 representing NM_001104577
  Red=Cloning site Green=Tags(s)
  
  MAAPTLGRLVLTHLLVALFGMGSWAAVNGIWVELPVVVKDLPEGWSLPSYLSVVVALGNLGLLVVTLWRQ
  LAPGKGEQVPIQVVQVLSVVGTALLAPLWHHVAPVAGQLHSVAFLTLALVLAMACCTSNVTFLPFLSHLP
  PPFLRSFFLGQGLSALLPCVLALVQGVGRLECPPAPTNGTSGPPLDFPERFPASTFFWALTALLVTSAAA
  FRGLLLLLPSLPSVTTGGSGPELQLGSPGAEEEEKEEEEALPLQEPPSQAAGTIPGPDPEAHQLFSAHGA
  FLLGLMAFTSAVTNGVLPSVQSFSCLPYGRLAYHLAVVLGSAANPLACFLAMGVLCRSLAGLVGLSLLGM
  LFGAYLMALAILSPCPPLVGTTAGVVLVVLSWVLCLCVFSYVKVAASSLLHGGGRPALLAAGVAIQVGSL
  LGAGAMFPPTSIYHVFQSRKDCVDPCGP
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001104577
• ORF Size: 1344 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001104577.1, NP_001098047.1
• RefSeq Size: 2429 bp
• RefSeq ORF: 1347 bp
• Locus ID: 55065
• UniProt ID: Q9NWF4
• Cytogenetics: 17p13.2
• Protein Families: Druggable Genome, GPCR, Transmembrane
• Gene Summary: Biological redox reactions require electron donors and acceptor. Vitamin B2 is the source for the flavin in flavin adenine dinucleotide (FAD) and flavin mononucleotide (FMN) which are common redox reagents. This gene encodes a member of the riboflavin (vitamin B2) transporter family. Haploinsufficiency of this protein can cause maternal riboflavin deficiency. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Jan 2013]