LRIT3 (NM_198506) Human Tagged ORF Clone

CAT#: RG222748

LRIT3 (tGFP-tagged) - Human leucine-rich repeat, immunoglobulin-like and transmembrane domains 3 (LRIT3)

Product Images

Circular map for RG222748

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: LRIT3
• Synonyms: CSNB1F; FIGLER4
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG222748 representing NM_198506
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCATCTCTTTGCATGTCTGTGCATTGTCCTTAGCTTTTTGGAAGGAGTGGGCTGTTTGTGTCCTTCAC
  AGTGCACCTGTGATTATCACGGCAGAAATGACGGCTCAGGATCAAGGTTGGTGCTATGTAATGACATGGA
  TATGAACGAGCTGCCTACGAACCTCCCCGTGGACACTGTGAAGCTTCGCATAGAGAAGACTGTCATCCGC
  AGAATCTCTGCGGAGGCCTTCTATTACCTGGTGGAGCTCCAGTATCTCTGGGTGACTTACAATTCCGTGG
  CCAGCATTGACCCCAGCAGCTTTTACAACCTGAAGCAACTGCATGAGTTGCGCTTGGATGGGAATTCTCT
  GGCTGCTTTCCCTTGGGCATCTCTGCTGGACATGCCCCTTCTGAGGACCCTGGACTTGCACAATAACAAA
  ATAACCAGTGTGCCAAATGAGGCGCTCAGGTATCTGAAGAACCTTGCCTACTTGGATTTATCAAGCAACA
  GACTCACCACATTGCCACCAGATTTCCTGGAGAGCTGGACTCATTTAGTTTCAACACCTTCTGGAGTCCT
  GGACCTTTCCCCAAGCAGGATTATTCTTGGTCTACAGGACAATCCTTGGTTCTGTGACTGTCATATTTCC
  AAAATGATTGAGTTGTCAAAGGTCGTTGACCCTGCTATAGTGCTTCTGGATCCACTGATGACTTGCAGTG
  AACCTGAGCGCCTCACAGGAATTTTGTTTCAGCGGGCTGAATTGGAGCATTGTCTGAAACCATCAGTGAT
  GACCTCAGCCACCAAAATCATGTCTGCTCTGGGCAGTAATGTTCTACTGCGGTGTGATGCCACTGGCTTC
  CCCACCCCACAGATCACATGGACCAGATCTGACAGCTCGCCAGTTAATTATACAGTAATACAAGAATCTC
  CAGAGGAAGGAGTCAGATGGTCCATAATGAGCTTGACAGGCATTTCTTCCAAAGACGCTGGGGATTACAA
  ATGTAAGGCCAAAAATCTGGCTGGGATGTCAGAAGCTGTGGTTACTGTGACAGTGCTTGGCATTACCACA
  ACTCCAATACCACCAGACACTTCTGAAAGAACTGGAGATCATCCTGAGTGGGATGTCCAGCCGGGATCTG
  GAAGATCTACATCTGTATCTAGCGCATCATCATATCTTTGGTCCTCTTCCTTCTCCCCCACATCTTCTTT
  TTCTGCTTCTACTTTGTCTCCTCCCTCTACTGCTTCCTTCTCTTTATCTCCTTTCTCCTCCTCCACTGTT
  TCTTCAACCACAACTCTGAGCACAAGCATCTCAGCAAGTACCACCATGGCCAACAAGCGATCATTCCAGC
  TCCACCAAGGTGGGAAAAGAAATTTAAAGGTGGCAAAGAATGGAAGTAAGCTTCCTCCAGCCAGCACAAG
  TAAGAAAGAAGAGCTGGCATTGTTGGATCAAACAATGCTTACGGAGACAAATGCCGCAATAGAAAACCTC
  AGGGTGGTCAGTGAGACTAAAGAGAGTGTGACATTGACGTGGAATATGATCAACACCACACATAACTCTG
  CAGTGACTGTGTTGTATTCCAAGTATGGTGGGAAGGACCTGCTGCTGTTGAATGCAGACTCCAGCAAGAA
  CCAAGTAACCATAGATGGCTTGGAACCCGGTGGGCAATACATGGCCTGTGTCTGTCCAAAAGGAGTGCCT
  CCCCAGAAAGACCAATGCATCACCTTTTCTACTGAAAGAGTTGAAGGAGATGATTCTCAATGGTCTCTCC
  TTCTCGTGGTGACCAGTACTGCCTGTGTTGTTATCTTACCATTGATTTGTTTCTTGTTGTACAAAGTTTG
  CAAACTGCAATGTAAATCAGAACCTTTTTGGGAAGATGATTTGGCAAAGGAGACTTATATCCAATTTGAG
  ACCCTGTTTCCCAGGTCTCAAAGTGTAGGTGAGCTCTGGACACGAAGCCACAGGGATGACTCAGAGAAAT
  TGCTGCTTTGTTCTAGGTCAAGTGTGGAATCTCAGGTGACTTTTAAAAGTGAAGGTTCCAGACCAGAGTA
  TTATTGC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG222748 representing NM_198506
  Red=Cloning site Green=Tags(s)
  
  MHLFACLCIVLSFLEGVGCLCPSQCTCDYHGRNDGSGSRLVLCNDMDMNELPTNLPVDTVKLRIEKTVIR
  RISAEAFYYLVELQYLWVTYNSVASIDPSSFYNLKQLHELRLDGNSLAAFPWASLLDMPLLRTLDLHNNK
  ITSVPNEALRYLKNLAYLDLSSNRLTTLPPDFLESWTHLVSTPSGVLDLSPSRIILGLQDNPWFCDCHIS
  KMIELSKVVDPAIVLLDPLMTCSEPERLTGILFQRAELEHCLKPSVMTSATKIMSALGSNVLLRCDATGF
  PTPQITWTRSDSSPVNYTVIQESPEEGVRWSIMSLTGISSKDAGDYKCKAKNLAGMSEAVVTVTVLGITT
  TPIPPDTSERTGDHPEWDVQPGSGRSTSVSSASSYLWSSSFSPTSSFSASTLSPPSTASFSLSPFSSSTV
  SSTTTLSTSISASTTMANKRSFQLHQGGKRNLKVAKNGSKLPPASTSKKEELALLDQTMLTETNAAIENL
  RVVSETKESVTLTWNMINTTHNSAVTVLYSKYGGKDLLLLNADSSKNQVTIDGLEPGGQYMACVCPKGVP
  PQKDQCITFSTERVEGDDSQWSLLLVVTSTACVVILPLICFLLYKVCKLQCKSEPFWEDDLAKETYIQFE
  TLFPRSQSVGELWTRSHRDDSEKLLLCSRSSVESQVTFKSEGSRPEYYC
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_198506
• ORF Size: 2037 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_198506.5
• RefSeq Size: 3641 bp
• RefSeq ORF: 2040 bp
• Locus ID: 345193
• UniProt ID: Q3SXY7
• Cytogenetics: 4q25
• Protein Families: Transmembrane
• Gene Summary: This gene encodes a protein that has a fibronectin type III domain and a C-terminal transmembrane domain, as well as a leucine-rich repeat domain and immunoglobulin-like domain near the N-terminus. The encoded protein may regulate fibroblast growth factor receptors and affect the modification of these receptors, which are glycosylated differently in the Golgi and endoplasmic reticulum. Mutations in this gene are associated with congenital stationary night blindness, type 1F. [provided by RefSeq, May 2013]