G protein regulated inducer of neurite outgrowth 2 (GPRIN2) (NM_014696) Human Tagged ORF Clone

CAT#: RG220773

GPRIN2 (tGFP-tagged) - Human G protein regulated inducer of neurite outgrowth 2 (GPRIN2)

Product Images

Circular map for RG220773

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: G protein regulated inducer of neurite outgrowth 2
• Synonyms: GRIN2; KIAA0514
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG220773 representing NM_014696
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCTCCAGCCACCCCGAGCCGGGTCCCTGGGCACCCCTGAGCCCCCGCCTTCAGCCCCTGTCCCAGA
  GCTCTTCCAGCCTGCTGGGTGAAGGCCGGGAACAGAGGCCAGAGCTCCACAAGACTGCCAGCAGCACCAT
  GTGGCAGGCCCAGCTGGGCGAGGCCAGCACCAGACCCCAGGCCCCGGAGGAAGAGGGGAACCCGCCTGAG
  AGCATGAAGCCAGCACGGGCCTCTGGCCCCAAGGCGCGACCCAGTGCTGGAGGCCACTGGCGGAGCAGCA
  CTGTGGGCAATGTGTCCACCATGGGCGGTGGTGACCTTTGTCGCCTGCGGGCCCCTAGTGCTGCTGCTAT
  GCAGAGGAGCCATTCAGACCTGGTCCGTAGCACCCAGATGCGGGGACACAGTGGTGCTCGGAAGGCCAGT
  CTCAGCTGCTCAGCCCTTGGCAGCAGCCCTGTCCACAGGGCTCAGCTGCAGCCAGGTGGTACTTCTGGCC
  AGGGTGGCCAGGCCCCTGCAGGCCTGGAAAGGGACCTGGCTCCTGAGGATGAGACTTCTAACTCAGCCTG
  GATGCTGGGGGCGAGTCAGTTGTCGGTGCCACCACTAGACCTGGGGGACACAACTGCCCACAGCAGCAGT
  GCCCAGGCTGAGCCCAAAGCTGCTGAACAGCTGGCTACCACCACCTGCCATGCTCTGCCCCCAGCTGCTC
  TACTCTGTGGCATGAGGGAGATGAGGGAGGTGGGGGCTGGTGGCTGCTGCCATGCCCTACCTGCCACAGG
  GATCCTGGCCTTTCCCAAACTAGTGGCGTCAGTGAGCGAGTCTGGGCTGCAGGCTCAGCATGGGGTGAAG
  ATCCACTGTAGGTTGTCTGGGGGGCTCCCTGGGCACTCCCATTGCTGTGCCCACCTTTGGGGTCCCGCTG
  GGTTAGTCCCAGAGCCTGGCTCTAGGACCAAAGATGTGTGGACCATGACCTCAGCCAATGACTTGGCCCC
  TGCAGAGGCATCCCCGCTGTCAGCCCAGGATGCTGGTGTGCAGGCGGCCCCAGTGGCGGCCTGCAAGGCT
  GTGGCCACCAGTCCGTCCCTGGAAGCGCCTGCAGCCCTGCATGTGTTCCCAGAGGTAACTCTGGGGTCCA
  GCCTGGAGGAGGCGCCGTCCCCTGTGCGGGATGTGCGATGGGATGCTGAGGGCATGACATGGGAGGTGTA
  CGGAGCTGCGGTGGACCCGGAGGTGCTCGGTGTGGCCATCCAGAAGCACCTGGAGATGCAGTTTGAGCAG
  CTGCAGCGGGCGCCTGCCAGCGAGGACAGCCTGTCTGTGGAGGGCCGGAGGGGGCCGCTGCGGGCTGTCA
  TGCAGTCCCTGCGGCGCCCCAGCTGCTGCGGCTGCTCTGGCGCGGCCCCCGAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG220773 representing NM_014696
  Red=Cloning site Green=Tags(s)
  
  MSSSHPEPGPWAPLSPRLQPLSQSSSSLLGEGREQRPELHKTASSTMWQAQLGEASTRPQAPEEEGNPPE
  SMKPARASGPKARPSAGGHWRSSTVGNVSTMGGGDLCRLRAPSAAAMQRSHSDLVRSTQMRGHSGARKAS
  LSCSALGSSPVHRAQLQPGGTSGQGGQAPAGLERDLAPEDETSNSAWMLGASQLSVPPLDLGDTTAHSSS
  AQAEPKAAEQLATTTCHALPPAALLCGMREMREVGAGGCCHALPATGILAFPKLVASVSESGLQAQHGVK
  IHCRLSGGLPGHSHCCAHLWGPAGLVPEPGSRTKDVWTMTSANDLAPAEASPLSAQDAGVQAAPVAACKA
  VATSPSLEAPAALHVFPEVTLGSSLEEAPSPVRDVRWDAEGMTWEVYGAAVDPEVLGVAIQKHLEMQFEQ
  LQRAPASEDSLSVEGRRGPLRAVMQSLRRPSCCGCSGAAPE
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_014696
• ORF Size: 1383 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014696.2, NP_055511.1
• RefSeq Size: 1896 bp
• RefSeq ORF: 1377 bp
• Locus ID: 9721
• UniProt ID: O60269
• Cytogenetics: 10q11.22
• Protein Families: Stem cell - Pluripotency
• Gene Summary: May be involved in neurite outgrowth.[UniProtKB/Swiss-Prot Function]