C10orf30 (BEND7) (NM_001100912) Human Tagged ORF Clone

CAT#: RG215854

BEND7 (tGFP-tagged) - Human BEN domain containing 7 (BEND7), transcript variant 2

Product Images

Circular map for RG215854

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: C10orf30
• Synonyms: C10orf30
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG215854 representing NM_001100912
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAATAAAAAAGCAAATTACAGGGATGAGAAGATTGCTGAACGACAGCACTGGGCGGATCTATCAGC
  GAGTTGGCAAAGAAGGAGAGAAACTAAAAGAAGAGCCCCAGGACCTGGATTTAGTCTGGCCTCCACGTTT
  GAACTCCTCTGCTGAGGCCCCGCAAAGCCTCCACCCGTCTTCACGTGGTGTGTGGAATGAGCTACCGCCC
  CAGAGTGGACAGTTCTCAGGGCAGTATGGCACCCGTTCTAGAACCTTCCAAAGCCAGCCCCACCCTACCA
  CGAGCTCCAATGGTATGGTTGTCAACAAACATTCAGAAGGCTCTCATGGGGGAGAACTTCCAGTGGTGAA
  TTCATCAGCTGGATCAAACTGCTGTACTTGTAACTGCCAGTCAACGTTGCAGGCCATTCTACAAGAACTC
  AAGACCATGAGGAAATTAATGCAAATTCAAGCAGTTGGAACTCAAAACAGACAACAACCTCCAATTTCCC
  TTATATGCTCCCAGCGAACTGCTGTCTCACGAAAGAGAAATAAAAAGAAAAAAGTGCCCCCAAAGACTGT
  GGAACCTCTTACTGTGAAACAGAAGCCCAGTGGGTCAGAGATGGAGAAAAAGTCGGTGGTGGCCTCTGAG
  CTATCTGCTCTCCAGGCAGCCGAGCACACCTCCCCGGAGGAGAGCCGCGTTCTAGGATTCGGCATTGTTC
  TGGAATCACCTTCCTCAGATCCAGAAGTACAACTTGCTGAAGGCTTTGACGTGTTTATGCCTAAATCTCA
  GCTGGACTCTATATTGTCAAACTACACTCGCTCAGGAAGCCTTCTGTTTAGAAAACTGGTGTGTGCGTTT
  TTTGATGACAAGACTTTGGCTAACTCCTTACCCAATGGGAAGAGGAAAAGAGGACTCAATGACAACCGGA
  AAGGACTAGACCAAAATATTGTGGGTGCAATAAAAGTCTTCACAGAAAAGTACTGTACAGCTAACCATGT
  GGATAAGCTTCCTGGCCCAAGAGATTGGGTACAGATTCTACAGGATCAAATTAAACTGGCCAGAAGAAGG
  TTAAAAAGAGGCTCAGAGATCGCGGACAGTGATGAAAGACTGGACGGCATTGCTCTACCACCAACAGTGG
  TC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG215854 representing NM_001100912
  Red=Cloning site Green=Tags(s)
  
  MEIKKQITGMRRLLNDSTGRIYQRVGKEGEKLKEEPQDLDLVWPPRLNSSAEAPQSLHPSSRGVWNELPP
  QSGQFSGQYGTRSRTFQSQPHPTTSSNGMVVNKHSEGSHGGELPVVNSSAGSNCCTCNCQSTLQAILQEL
  KTMRKLMQIQAVGTQNRQQPPISLICSQRTAVSRKRNKKKKVPPKTVEPLTVKQKPSGSEMEKKSVVASE
  LSALQAAEHTSPEESRVLGFGIVLESPSSDPEVQLAEGFDVFMPKSQLDSILSNYTRSGSLLFRKLVCAF
  FDDKTLANSLPNGKRKRGLNDNRKGLDQNIVGAIKVFTEKYCTANHVDKLPGPRDWVQILQDQIKLARRR
  LKRGSEIADSDERLDGIALPPTVV
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001100912
• ORF Size: 1122 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001100912.2
• RefSeq Size: 1924 bp
• RefSeq ORF: 1125 bp
• Locus ID: 222389
• UniProt ID: Q8N7W2
• Cytogenetics: 10p13