LRRC71 (NM_144702) Human Tagged ORF Clone

CAT#: RG213168

LRRC71 (tGFP-tagged) - Human leucine rich repeat containing 71 (LRRC71)

Product Images

Circular map for RG213168

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: LRRC71
• Synonyms: C1orf92
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG213168 representing NM_144702
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGGCGCGCCC
  
  ATGTCGAGCGAGCAGAGCGCGCCGGGGGCCTCACCCAGGGCCCCGCGTCCGGGGACCCAGAAGTCTTCTG
  GCGCGGTGACCAAAAAGGGAGAGCGCGCGGCCAAAGAGAAGCCAGCGACCGTTCTGCCTCCCGTGGGGGA
  GGAGGAGCCCAAAAGCCCTGAGGAGTACCAGTGCTCCGGGGTCCTCGAGACCGACTTCGCCGAGCTCTGC
  ACGCGGTGGGGCTACACGGACTTCCCCAAAGTTGTCAACCGGCCCCGCCCCCACCCGCCCTTCGTCCCCT
  CCGCCTCTTTGTCGGAAAAGGCCACCTTAGACGATCCGCGGCTGTCGGGGTCCTGCAGCCTCAATAGCCT
  GGAGAGCAAATACGTGTTCTTCCGGCCCACCATCCAGGTGGAGCTGGAGCAGGAGGACAGCAAGTCAGTG
  AAGGAAATCTACATCCGCGGTTGGAAGGTTGAGGAACGGATTCTGGGTGTCTTCTCTAAATGTCTGCCCC
  CGCTTACCCAGCTACAGGCCATCAACTTGTGGAAGGTGGGGCTGACCGATAAGACCCTGACCACCTTCAT
  CGAGCTCCTGCCTCTCTGTTCATCCACGCTCAGGAAGGTGTCTCTGGAGGGGAACCCACTGCCGGAGCAG
  TCCTATCACAAGCTCATGGCCTTGGACAGCACGATTGCGCACTTGTCTCTGCGGAACAATAACATCGACG
  ACCGCGGGGCGCAACTCCTGGGCCAGGCGCTGTCCACGCTGCACAGCTGCAACCGGACCCTCGTCTCGCT
  CAACCTGGGTTTCAACCACATCGGTGACGAGGGCGCAGGCTACATCGCGGACGGCCTCCGGCTGAACCGT
  TCCCTGCTCTGGCTGTCCCTGGCCCACAACCGCATCCAGGACAAGGGCGCCCTGAAGCTGGCTGAGGTCC
  TGCGCGCCTTCGAGCTGACACACACCGAAGTGGTGGAGCGCCGACGCCTCCTGCTGGAAAAAGGGACACA
  GGAGCGCTCGCGATCGCCCTCCTCCTCTCGACACGGGGACTCCAAAACGGACCGTGAGAAGAGTCAGATG
  GTAGGGATCAGCAATAGTGCATTGGTGGACAAGACAGACAAGACGCAGACAATGAAAACCCCTAAGGGCC
  TGGGCAAGAAAAAGGAGAAATCATGGGAATTGGCCAAGAAAGAGGAGAAGTTGGGGTCTGGGCAGTCACC
  CACACAAGGAACCCCTAAGAAGGAAGATGCCACAAAGGCAGGCAAGGGGAAGGTAACCATCCCTGAACAG
  AAGCCAAGCAGGGCAAAAGGGATCAAGATCGGGAGCAGAGAGAAGCGCAGCATCCTCCTGGAGTCCGAGC
  TGGTTGTTGAGGCTACTGAGGTGGTCAACCCTCTCCTGGAGCCTGTGGAGCACCGAGATGGGAAAGTTTT
  CATGCCTGGGAACAAGGTCCTTTTGCACCTCAACCTCATCCGGAACCGCATCACAGAGGTGGGGCTGGAG
  GGCTTCCTCGCCACAGTGCAGTATCAGATGCAGTTCTCCAAGGCCAAGAGTGCATCCAAGGGTCCAGTGG
  GGCTGCTGTGGCTGTCCCTGGCTAAAAATTGCTTCGCCCCACAATGTCCTGCGTACGCCATAATCCAGGA
  GCTGATGTTGCCAAGGGATCCCATCAAGGCCAAACTCAGGGAGGATGAGGCCATGGCATTCTTCCCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG213168 representing NM_144702
  Red=Cloning site Green=Tags(s)
  
  MSSEQSAPGASPRAPRPGTQKSSGAVTKKGERAAKEKPATVLPPVGEEEPKSPEEYQCSGVLETDFAELC
  TRWGYTDFPKVVNRPRPHPPFVPSASLSEKATLDDPRLSGSCSLNSLESKYVFFRPTIQVELEQEDSKSV
  KEIYIRGWKVEERILGVFSKCLPPLTQLQAINLWKVGLTDKTLTTFIELLPLCSSTLRKVSLEGNPLPEQ
  SYHKLMALDSTIAHLSLRNNNIDDRGAQLLGQALSTLHSCNRTLVSLNLGFNHIGDEGAGYIADGLRLNR
  SLLWLSLAHNRIQDKGALKLAEVLRAFELTHTEVVERRRLLLEKGTQERSRSPSSSRHGDSKTDREKSQM
  VGISNSALVDKTDKTQTMKTPKGLGKKKEKSWELAKKEEKLGSGQSPTQGTPKKEDATKAGKGKVTIPEQ
  KPSRAKGIKIGSREKRSILLESELVVEATEVVNPLLEPVEHRDGKVFMPGNKVLLHLNLIRNRITEVGLE
  GFLATVQYQMQFSKAKSASKGPVGLLWLSLAKNCFAPQCPAYAIIQELMLPRDPIKAKLREDEAMAFFP
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: AscI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_144702
• ORF Size: 1677 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_144702.3
• RefSeq Size: 1989 bp
• RefSeq ORF: 1680 bp
• Locus ID: 149499
• UniProt ID: Q8N4P6
• Cytogenetics: 1q23.1