IL16 (NM_172217) Human Tagged ORF Clone

CAT#: RG212217

IL16 (tGFP-tagged) - Human interleukin 16 (lymphocyte chemoattractant factor) (IL16), transcript variant 2

Product Images

Circular map for RG212217

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: IL16
• Synonyms: LCF; NIL16; prIL-16; PRIL16
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG212217 representing NM_172217
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGTCGCACAGCCGCGCTGGAAAGAGCAGAAAATCTGCAAAATTTCGGTCCATCTCCAGGTCCCTGA
  TGCTCTGTAATGCTAAGACCAGTGATGATGGCTCTAGCCCTGATGAGAAATATCCTGATCCCTTTGAGAT
  TTCCTTGGCCCAGGGCAAGGAGGGAATTTTCCACTCATCTGTGCAGCTGGCAGACACATCGGAGGCTGGG
  CCCAGCAGTGTTCCTGATCTAGCACTGGCCTCGGAGGCTGCTCAACTCCAAGCAGCTGGGAATGATCGAG
  GCAAGACCTGTAGGAGGATATTCTTCATGAAGGAATCTTCCACAGCTTCCTCTCGAGAAAAGCCTGGAAA
  ACTAGAAGCACAAAGTAGTAACTTCCTGTTTCCTAAAGCCTGCCACCAAAGGGCACGCAGCAACTCAACC
  AGTGTTAATCCCTATTGCACAAGAGAAATTGATTTTCCAATGACCAAGAAATCTGCAGCGCCCACGGACA
  GGCAGCCTTACTCTCTCTGCAGTAACAGGAAGTCCCTCTCTCAACAATTGGACTGTCCAGCAGGAAAGGC
  TGCGGGAACTTCGAGACCAACACGGTCCCTGAGCACAGCTCAGCTCGTGCAGCCATCTGGGGGCCTCCAG
  GCTTCAGTCATCTCCAACATCGTGCTGATGAAGGGCCAGGCTAAGGGTCTGGGCTTCAGCATCGTTGGGG
  GAAAAGACAGCATTTATGGCCCCATTGGGATTTACGTCAAAACCATTTTTGCAGGGGGAGCAGCAGCAGC
  CGATGGAAGGCTACAGGAAGGTGATGAAATTCTGGAGCTCAATGGTGAATCAATGGCTGGACTAACACAT
  CAGGATGCTTTGCAGAAGTTCAAGCAAGCCAAAAAGGGGCTCCTCACCCTCACCGTGAGAACCCGCCTGA
  CGGCGCCTCCTTCCCTGTGCAGCCACCTGTCTCCCCCACTGTGCCGCTCCCTGAGCTCCAGCACTTGTAT
  CACCAAGGACAGCAGCTCCTTCGCCTTGGAAAGCCCCTCGGCTCCCATCAGCACCGCCAAGCCCAATTAC
  AGAATCATGGTGGAGGTTTCTCTGCAGAAAGAGGCCGGCGTGGGCCTGGGCATCGGCCTGTGCAGCGTTC
  CCTACTTCCAATGCATCTCTGGCATTTTCGTCCACACGCTGTCACCAGGATCCGTGGCGCACCTGGACGG
  ACGTCTCCGGTGTGGGGACGAGATTGTGGAAATCAGTGATTCCCCTGTGCACTGCCTGACGCTCAATGAA
  GTCTACACGATCCTGAGTCACTGTGATCCCGGTCCAGTCCCCATCATTGTTAGCCGACATCCAGACCCAC
  AGGTCTCTGAACAGCAACTCAAAGAAGCTGTGGCCCAGGCTGTGGAAAACACCAAGTTTGGAAAGGAGAG
  GCATCAGTGGAGTCTGGAAGGTGTCAAAAGGCTGGAAAGCAGTTGGCACGGGCGGCCCACCTTGGAGAAG
  GAACGAGAGAAGAACTCAGCACCCCCGCATCGCAGGGCTCAGAAGGTCATGATCCGCTCCAGCAGTGACA
  GCAGCTACATGTCTGGGTCCCCAGGGGGAAGTCCTGGGAGTGGCAGTGCTGAGAAGCCGTCCTCTGACGT
  GGACATCAGCACACACAGCCCCAGCTTGCCTCTGGCACGGGAGCCAGTGGTGCTTTCTATAGCATCCTCC
  AGGCTGCCCCAGGAGAGCCCACCCCTCCCAGAGAGCCGGGACAGCCACCCGCCGCTGAGACTGAAGAAAT
  CCTTTGAGATTTTGGTGAGAAAGCCTATGTCCTCCAAGCCCAAGCCTCCACCCAGAAAATACTTTAAAAG
  TGACAGTGACCCTCAGAAGAGTCTGGAAGAGAGAGAGAACTCCTCATGCTCTTCTGGGCACACCCCACCC
  ACCTGTGGCCAGGAAGCGAGAGAGCTGCTGCCACTGCTGCTACCACAGGAAGACACAGCAGGGAGAAGCC
  CTAGTGCCTCTGCCGGCTGCCCAGGACCTGGTATCGGCCCACAGACCAAGTCCTCCACAGAGGGCGAGCC
  AGGGTGGAGAAGAGCCAGCCCAGTGACCCAAACATCCCCGATAAAACACCCACTGCTTAAGAGGCAGGCT
  CGGATGGACTATAGCTTTGATACCACAGCCGAAGACCCTTGGGTTAGGATTTCTGACTGCATCAAAAACT
  TATTTAGCCCCATCATGAGTGAGAACCATGGCCACATGCCTCTACAGCCCAATGCCAGCCTGAATGAAGA
  AGAAGGGACACAGGGCCACCCAGATGGGACCCCACCAAAGCTGGACACCGCCAATGGCACTCCCAAAGTT
  TACAAGTCAGCAGACAGCAGCACTGTGAAGAAAGGTCCTCCTGTGGCTCCCAAGCCAGCCTGGTTTCGCC
  AAAGCTTGAAAGGTTTGAGGAATCGTGCTTCAGACCCAAGAGGGCTCCCTGATCCTGCCTTGTCCACCCA
  GCCAGCACCTGCTTCCAGGGAGCACCTAGGATCACACATCCGGGCCTCCTCCTCCTCCTCCTCCATCAGG
  CAGAGAATCAGCTCCTTTGAAACCTTTGGCTCCTCTCAACTGCCTGACAAAGGAGCCCAGAGACTGAGCC
  TCCAGCCCTCCTCTGGGGAGGCAGCAAAACCTCTTGGGAAGCATGAGGAAGGACGGTTTTCTGGACTCTT
  GGGGCGAGGGGCTGCACCCACTCTTGTGCCCCAGCAGCCTGAGCAAGTACTGTCCTCGGGGTCCCCTGCA
  GCCTCCGAGGCCAGAGACCCAGGTGTGTCTGAGTCCCCTCCCCCAGGGCGGCAGCCCAATCAGAAAACTC
  TCCCCCCTGGCCCGGACCCGCTCCTAAGGCTGCTGTCAACACAGGCTGAGGAATCTCAAGGCCCAGTGCT
  CAAGATGCCTAGCCAGCGAGCACGGAGCTTCCCCCTGACCAGGTCCCAGTCCTGTGAGACGAAGCTACTT
  GACGAAAAGACCAGCAAACTCTATTCTATCAGCAGCCAAGTGTCATCGGCTGTCATGAAATCCTTGCTGT
  GCCTTCCATCTTCTATCTCCTGTGCCCAGACTCCCTGCATCCCCAAGGAAGGGGCATCTCCAACATCATC
  ATCCAACGAAGACTCAGCTGCAAATGGTTCTGCTGAAACATCTGCCTTGGACACAGGGTTCTCGCTCAAC
  CTTTCAGAGCTGAGAGAATATACAGAGGGTCTCACGGAAGCCAAGGAAGACGATGATGGGGACCACAGTT
  CCCTTCAGTCTGGTCAGTCCGTTATCTCCCTGCTGAGCTCAGAAGAATTAAAAAAACTCATCGAGGAGGT
  GAAGGTTCTGGATGAAGCAACATTAAAGCAATTAGACGGCATCCATGTCACCATCTTACACAAGGAGGAA
  GGTGCTGGTCTTGGGTTCAGCTTGGCAGGAGGAGCAGATCTAGAAAACAAGGTGATTACGGTTCACAGAG
  TGTTTCCAAATGGGCTGGCCTCCCAGGAAGGGACTATTCAGAAGGGCAATGAGGTTCTTTCCATCAACGG
  CAAGTCTCTCAAGGGGACCACGCACCATGATGCCTTGGCCATCCTCCGCCAAGCTCGAGAGCCCAGGCAA
  GCTGTGATTGTCACAAGGAAGCTGACTCCAGAGGCCATGCCCGACCTCAACTCCTCCACTGACTCTGCAG
  CCTCAGCCTCTGCAGCCAGTGATGTTTCTGTAGAATCTACAGCAGAGGCCACAGTCTGCACGGTGACACT
  GGAGAAGATGTCGGCAGGGCTGGGCTTCAGCCTGGAAGGAGGGAAGGGCTCCCTACACGGAGACAAGCCT
  CTCACCATTAACAGGATTTTCAAAGGAGCAGCCTCAGAACAAAGTGAGACAGTCCAGCCTGGAGATGAAA
  TCTTGCAGCTGGGTGGCACTGCCATGCAGGGCCTCACACGGTTTGAAGCCTGGAACATCATCAAGGCACT
  GCCTGATGGACCTGTCACGATTGTCATCAGGAGAAAAAGCCTCCAGTCCAAGGAAACCACAGCTGCTGGA
  GACTCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG212217 representing NM_172217
  Red=Cloning site Green=Tags(s)
  
  MESHSRAGKSRKSAKFRSISRSLMLCNAKTSDDGSSPDEKYPDPFEISLAQGKEGIFHSSVQLADTSEAG
  PSSVPDLALASEAAQLQAAGNDRGKTCRRIFFMKESSTASSREKPGKLEAQSSNFLFPKACHQRARSNST
  SVNPYCTREIDFPMTKKSAAPTDRQPYSLCSNRKSLSQQLDCPAGKAAGTSRPTRSLSTAQLVQPSGGLQ
  ASVISNIVLMKGQAKGLGFSIVGGKDSIYGPIGIYVKTIFAGGAAAADGRLQEGDEILELNGESMAGLTH
  QDALQKFKQAKKGLLTLTVRTRLTAPPSLCSHLSPPLCRSLSSSTCITKDSSSFALESPSAPISTAKPNY
  RIMVEVSLQKEAGVGLGIGLCSVPYFQCISGIFVHTLSPGSVAHLDGRLRCGDEIVEISDSPVHCLTLNE
  VYTILSHCDPGPVPIIVSRHPDPQVSEQQLKEAVAQAVENTKFGKERHQWSLEGVKRLESSWHGRPTLEK
  EREKNSAPPHRRAQKVMIRSSSDSSYMSGSPGGSPGSGSAEKPSSDVDISTHSPSLPLAREPVVLSIASS
  RLPQESPPLPESRDSHPPLRLKKSFEILVRKPMSSKPKPPPRKYFKSDSDPQKSLEERENSSCSSGHTPP
  TCGQEARELLPLLLPQEDTAGRSPSASAGCPGPGIGPQTKSSTEGEPGWRRASPVTQTSPIKHPLLKRQA
  RMDYSFDTTAEDPWVRISDCIKNLFSPIMSENHGHMPLQPNASLNEEEGTQGHPDGTPPKLDTANGTPKV
  YKSADSSTVKKGPPVAPKPAWFRQSLKGLRNRASDPRGLPDPALSTQPAPASREHLGSHIRASSSSSSIR
  QRISSFETFGSSQLPDKGAQRLSLQPSSGEAAKPLGKHEEGRFSGLLGRGAAPTLVPQQPEQVLSSGSPA
  ASEARDPGVSESPPPGRQPNQKTLPPGPDPLLRLLSTQAEESQGPVLKMPSQRARSFPLTRSQSCETKLL
  DEKTSKLYSISSQVSSAVMKSLLCLPSSISCAQTPCIPKEGASPTSSSNEDSAANGSAETSALDTGFSLN
  LSELREYTEGLTEAKEDDDGDHSSLQSGQSVISLLSSEELKKLIEEVKVLDEATLKQLDGIHVTILHKEE
  GAGLGFSLAGGADLENKVITVHRVFPNGLASQEGTIQKGNEVLSINGKSLKGTTHHDALAILRQAREPRQ
  AVIVTRKLTPEAMPDLNSSTDSAASASAASDVSVESTAEATVCTVTLEKMSAGLGFSLEGGKGSLHGDKP
  LTINRIFKGAASEQSETVQPGDEILQLGGTAMQGLTRFEAWNIIKALPDGPVTIVIRRKSLQSKETTAAG
  DS
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_172217
• ORF Size: 3996 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_172217.4
• RefSeq Size: 8338 bp
• RefSeq ORF: 3999 bp
• Locus ID: 3603
• UniProt ID: Q14005
• Cytogenetics: 15q25.1
• Protein Families: Druggable Genome, Secreted Protein
• Gene Summary: The protein encoded by this gene is a pleiotropic cytokine that functions as a chemoattractant, a modulator of T cell activation, and an inhibitor of HIV replication. The signaling process of this cytokine is mediated by CD4. The product of this gene undergoes proteolytic processing, which is found to yield two functional proteins. The cytokine function is exclusively attributed to the secreted C-terminal peptide, while the N-terminal product may play a role in cell cycle control. Caspase 3 is reported to be involved in the proteolytic processing of this protein. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Feb 2010]