MAGEB18 (NM_173699) Human Tagged ORF Clone
CAT#: RG206329
- TrueORF®
MAGEB18 (tGFP-tagged) - Human melanoma antigen family B, 18 (MAGEB18)
ORF Plasmid: DDK
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
AAV Particle: DDK
"NM_173699" in other vectors (4)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Symbol | MAGEB18 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG206329 representing NM_173699
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCTCGAGGTCAGAAGAGTAAGCTCCGTGCCCGTGAGAAACGCCACCAGGCTCGTTGTGAGAATCAGG ATCTGGGAGCTACGCAGGCCACTGTGGCAGAAGGAGAGTCACCCTCCTCTGCCTATCTTCTCTTTGGTGA CAGACCCCAGAATTTGCCTGCTGCTGAGACACCTAGCATCCCTGAAGCGCTTCAGGGAGCCCCATCCACC ACCAATGCTATTGCACCTGTTTCATGCAGTTCAAATGAAGGTGCCAGCAGCCAAGATGAGAAAAGTCTAG GTTCCTCAAGGGAAGCTGAGGGCTGGAAAGAAGATCCTTTAAACAAGAAAGTAGTGTCGCTGGTGCATTT CTTGCTTCAGAAGTATGAAACGAAAGAGCCAATTACAAAGGGAGATATGATAAAGTTTGTTATCAGGAAG GATAAGTGTCACTTCAATGAGATCCTCAAGAGAGCCTCTGAGCACATGGAGCTGGCACTTGGTGTTGATT TGAAGGAAGTGGATCCCATCAGGCACTACTATGCCTTTTTCAGCAAATTAGACCTCACCTATGATGAAAC AACCAGTGATGAAGAAAAAATTCCCAAGACTGGCCTCCTGATGATTGCACTGGGTGTGATCTTTCTGAAT GGCAACCGTGCCCCAGAAGAGGCAGTCTGGGAAATTATGAATATGATGGGTGTATATGCTGATAGGAAGC ACTTCCTCTATGGGGATCCCAGGAAGGTCATGACCAAAGATTTGGTGCAGCTAAAGTACCTGGAGTACCA GCAAGTGCCCAACAGTGATCCTCCACGCTATGAATTCCTGTGGGGTCCAAGAGCTCACGCTGAAACTAGC AAGATGAAAGTCCTGGAGTTTGTAGCCAAGATACATGATACCGTCCCTAGTGCCTTCCCATCCTGCTATG AAGAGGCTTTGAGGGATGAGGAACAGAGAACCCAAGCCAGAGCTGCAGCCAGGGCTCATACTGCTGCCAT GGCAAATGCACGTTCCAGAACCACGTCTAGCAGCTTCTCCCATGCTAAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG206329 representing NM_173699
Red=Cloning site Green=Tags(s) MPRGQKSKLRAREKRHQARCENQDLGATQATVAEGESPSSAYLLFGDRPQNLPAAETPSIPEALQGAPST TNAIAPVSCSSNEGASSQDEKSLGSSREAEGWKEDPLNKKVVSLVHFLLQKYETKEPITKGDMIKFVIRK DKCHFNEILKRASEHMELALGVDLKEVDPIRHYYAFFSKLDLTYDETTSDEEKIPKTGLLMIALGVIFLN GNRAPEEAVWEIMNMMGVYADRKHFLYGDPRKVMTKDLVQLKYLEYQQVPNSDPPRYEFLWGPRAHAETS KMKVLEFVAKIHDTVPSAFPSCYEEALRDEEQRTQARAAARAHTAAMANARSRTTSSSFSHAK TRTRPLE - GFP Tag - V |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_173699 |
ORF Size | 1029 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_173699.2 |
RefSeq Size | 1827 bp |
RefSeq ORF | 1032 bp |
Locus ID | 286514 |
UniProt ID | Q96M61 |
Cytogenetics | Xp21.3 |
Gene Summary | May enhance ubiquitin ligase activity of RING-type zinc finger-containing E3 ubiquitin-protein ligases. Proposed to act through recruitment and/or stabilization of the Ubl-conjugating enzyme (E2) at the E3:substrate complex.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC206329 | MAGEB18 (Myc-DDK-tagged)-Human melanoma antigen family B, 18 (MAGEB18) |
USD 457.00 |
|
RC206329L3 | Lenti ORF clone of Human melanoma antigen family B, 18 (MAGEB18), Myc-DDK-tagged |
USD 757.00 |
|
RC206329L4 | Lenti ORF clone of Human melanoma antigen family B, 18 (MAGEB18), mGFP tagged |
USD 757.00 |
|
SC123333 | MAGEB18 (untagged)-Human melanoma antigen family B, 18 (MAGEB18) |
USD 457.00 |
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