Pit1 (POU1F1) (NM_001122757) Human Tagged ORF Clone
CAT#: RC225455
POU1F1 (Myc-DDK-tagged)-Human POU class 1 homeobox 1 (POU1F1), transcript variant beta
ORF Plasmid: tGFP
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
AAV Particle: DDK
"NM_001122757" in other vectors (4)
USD 198.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Pit1 |
Synonyms | CPHD1; GHF-1; Pit-1; PIT1; POU1F1a |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC225455 representing NM_001122757
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGTTGCCAAGCTTTTACTTCGGCTGATACCTTTATACCTCTGAATTCTGACGCCTCTGCAACTCTGC CTCTGATAATGCATCACAGTGCTGCCGAGTGTCTACCAGTCTCCAACCATGCCACCAATGTGATGTCTAC AGTCCCATCTATTTTGTCTTTGATCCAAACTCCTAAATGTTTGTGCACACATTTCTCGGTGACAACGTTG GGAAACACAGCAACAGGACTTCATTATTCTGTTCCTTCCTGTCATTATGGAAACCAGCCATCAACCTATG GAGTGATGGCAGGTAGTTTAACCCCTTGTCTTTATAAATTTCCTGACCACACCTTGAGTCATGGATTTCC TCCTATACACCAGCCTCTTCTGGCAGAGGACCCCACAGCTGCTGATTTCAAGCAGGAACTCAGGCGGAAA AGTAAATTGGTGGAAGAGCCAATAGACATGGATTCTCCAGAAATCAGAGAACTTGAAAAGTTTGCCAATG AATTTAAAGTGAGACGAATTAAATTAGGATACACCCAGACAAATGTTGGGGAGGCCCTGGCAGCTGTGCA TGGCTCTGAATTCAGTCAAACAACAATCTGCCGATTTGAAAATCTGCAGCTCAGCTTTAAAAATGCATGC AAACTGAAAGCAATATTATCCAAATGGCTGGAGGAAGCTGAGCAAGTAGGAGCTTTGTACAATGAAAAAG TGGGAGCAAATGAAAGGAAAAGAAAACGAAGAACAACTATAAGCATTGCTGCTAAAGATGCTCTGGAGAG ACACTTTGGAGAACAGAATAAACCTTCTTCTCAAGAGATCATGAGGATGGCTGAAGAACTGAATCTGGAG AAAGAAGTAGTAAGAGTTTGGTTTTGCAACCGGAGGCAGAGAGAAAAACGGGTGAAAACAAGTCTGAATC AGAGTTTATTTTCTATTTCTAAGGAACATCTTGAGTGCAGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC225455 representing NM_001122757
Red=Cloning site Green=Tags(s) MSCQAFTSADTFIPLNSDASATLPLIMHHSAAECLPVSNHATNVMSTVPSILSLIQTPKCLCTHFSVTTL GNTATGLHYSVPSCHYGNQPSTYGVMAGSLTPCLYKFPDHTLSHGFPPIHQPLLAEDPTAADFKQELRRK SKLVEEPIDMDSPEIRELEKFANEFKVRRIKLGYTQTNVGEALAAVHGSEFSQTTICRFENLQLSFKNAC KLKAILSKWLEEAEQVGALYNEKVGANERKRKRRTTISIAAKDALERHFGEQNKPSSQEIMRMAEELNLE KEVVRVWFCNRRQREKRVKTSLNQSLFSISKEHLECR TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_001122757 |
ORF Size | 951 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001122757.3 |
RefSeq ORF | 954 bp |
Locus ID | 5449 |
UniProt ID | P28069 |
Cytogenetics | 3p11.2 |
Protein Families | Druggable Genome, Transcription Factors |
MW | 35.5 kDa |
Gene Summary | This gene encodes a member of the POU family of transcription factors that regulate mammalian development. The protein regulates expression of several genes involved in pituitary development and hormone expression. Mutations in this genes result in combined pituitary hormone deficiency. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC225455L3 | Lenti ORF clone of Human POU class 1 homeobox 1 (POU1F1), transcript variant beta, Myc-DDK-tagged |
USD 600.00 |
|
RC225455L4 | Lenti ORF clone of Human POU class 1 homeobox 1 (POU1F1), transcript variant beta, mGFP tagged |
USD 600.00 |
|
RG225455 | POU1F1 (tGFP-tagged) - Human POU class 1 homeobox 1 (POU1F1), transcript variant beta |
USD 500.00 |
|
SC318829 | POU1F1 (untagged)-Human POU class 1 homeobox 1 (POU1F1), transcript variant beta |
USD 330.00 |
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