KSR2 (NM_173598) Human Tagged ORF Clone

CAT#: RC211665

KSR2 (Myc-DDK-tagged)-Human kinase suppressor of ras 2 (KSR2)

Product Images

Circular map for RC211665

• 

  Western blot validation of overexpression lysate (Cat# LY406499) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC211665 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: KSR2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC211665 representing NM_173598
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATAGACTTGAGCATCTCCAACCTGGAAGGGCTTAGGACCAAATGTGCTACCTCCAACGACCTCACAC
  AAAAAGAAATCCGGACCCTGGAGAGCAAGCTGGTGAAGTACTTCAGCCGGCAGCTGTCCTGCAAAAAGAA
  GGTAGCCTTGCAGGAGCGCAACGCGGAGCTGGACGGCTTCCCCCAGCTACGGCACTGGTTCCGAATCGTC
  GATGTGCGCAAGGAGGTCCTGGAGGAAATCTCCCCCGGCCAGCTGAGCCTGGAGGACCTCTTGGAGATGA
  CGGATGAACAGGTGTGCGAGACTGTGGAGAAATACGGAGCCAACCGGGAGGAGTGTGCCCGCCTCAACGC
  CTCCCTCTCCTGCCTCAGGAATGTCCACATGTCAGGAGGCAACCTTTCCAAACAAGACTGGACCATCCAG
  TGGCCCACGACAGAGACGGGGAAGGAGAACAATCCCGTGTGCCCCCCGGAGCCCACCCCGTGGATCCGCA
  CCCATCTCTCCCAGAGCCCCAGGGTCCCGTCCAAGTGCGTCCAGCACTATTGTCACACCAGCCCCACTCC
  CGGGGCCCCTGTGTACACCCACGTGGACAGGCTTACCGTGGACGCCTACCCGGGCTTGTGCCCGCCCCCG
  CCACTGGAGTCGGGCCACCGTTCCCTGCCCCCATCGCCCCGGCAGCGGCACGCGGTCCGCACCCCGCCGC
  GCACCCCCAACATCGTCACCACCGTGACCCCGCCGGGCACGCCGCCCATGAGGAAGAAGAACAAGCTGAA
  GCCCCCGGGGACCCCACCGCCCTCCTCCCGAAAACTGATACACTTGATCCCGGGATTCACCGCGCTGCAT
  CGGAGCAAATCCCACGAGTTCCAGCTGGGGCACCGCGTGGACGAGGCCCACACGCCCAAAGCCAAGAAGA
  AGAGCAAACCCTTGAACCTCAAGATCCACAGCAGCGTAGGCAGCTGCGAGAACATCCCCTCTCAGCAGCG
  CTCCCCGCTGCTGTCCGAGCGCTCCCTCCGCTCCTTCTTTGTGGGACACGCACCTTTCCTGCCTTCCACC
  CCTCCTGTTCACACTGAGGCCAACTTCTCTGCAAACACACTGTCAGTGCCACGCTGGTCCCCGCAGATCC
  CTCGCAGAGATCTCGGCAACTCCATCAAGCACAGGTTTTCCACCAAGTACTGGATGTCTCAGACGTGCAC
  AGTCTGTGGGAAAGGGATGCTTTTTGGCCTCAAGTGTAAAAACTGCAAGTTAAAGTGCCACAACAAATGC
  ACCAAAGAAGCCCCACCCTGTCATCTTCTGATCATCCACCGAGGAGATCCAGCAAGGTTAGTCCGGACAG
  AGTCCGTTCCGTGTGACATCAACAACCCTCTACGGAAGCCACCTCGCTATTCAGACCTGCACATCAGTCA
  GACGCTCCCCAAAACCAACAAAATCAACAAGGACCACATCCCTGTCCCTTACCAGCCAGACTCCAGCAGC
  AACCCCTCCTCCACGACGTCCTCCACGCCCTCCTCGCCAGCACCCCCCCTCCCTCCTAGTGCCACGCCGC
  CTTCTCCCCTACACCCTTCCCCACAGTGCACACGGCAGCAGAAGAACTTCAACCTGCCAGCATCCCACTA
  CTACAAATACAAGCAGCAGTTCATCTTCCCAGATGTGGTGCCGGTGCCGGAGACGCCGACCCGGGCGCCC
  CAGGTCATCCTGCATCCGGTGACCTCGAATCCAATCTTGGAAGGAAATCCATTACTTCAAATTGAAGTGG
  AGCCAACGTCGGAGAATGAAGAGGTCCATGATGAGGCCGAAGAGTCAGAGGATGACTTCGAGGAGATGAA
  CCTGTCCCTCCTCTCGGCCCGGAGCTTCCCACGCAAGGCCAGCCAGACCAGCATCTTCCTTCAGGAGTGG
  GACATCCCCTTTGAGCAGCTGGAGATCGGCGAGCTCATTGGAAAGGGCCGCTTTGGGCAAGTGTACCACG
  GCCGCTGGCATGGCGAGGTGGCCATCCGGCTGATTGACATTGAGAGGGACAACGAGGACCAGCTCAAGGC
  CTTCAAGCGGGAGGTGATGGCCTACAGGCAGACACGGCATGAGAACGTGGTGCTTTTCATGGGTGCCTGC
  ATGAGCCCGCCTCACCTGGCCATCATCACCAGCCTCTGTAAGGGACGGACGCTCTATTCCGTTGTGAGGG
  ATGCCAAAATCGTTTTGGATGTCAACAAAACCAGGCAGATTGCTCAAGAAATTGTGAAGGGCATGGGCTA
  CCTCCACGCCAAGGGAATCCTACACAAGGACCTCAAGTCAAAGAACGTCTTCTATGACAACGGCAAAGTG
  GTCATCACGGACTTTGGACTCTTCAGCATTTCTGGGGTGCTGCAGGCTGGCAGGCGGGAGGACAAACTGC
  GCATCCAGAATGGCTGGCTATGCCACCTGGCACCAGAGATCATCCGCCAGCTGTCCCCCGACACAGAGGA
  GGATAAGCTCCCCTTCTCCAAGCACTCTGACGTCTTTGCCCTTGGCACAATCTGGTATGAACTCCACGCC
  AGGGAATGGCCTTTCAAGACCCAACCAGCAGAGGCAATAATCTGGCAAATGGGCACAGGCATGAAACCCA
  ACCTCAGCCAGATTGGCATGGGAAAAGAAATCTCGGACATTCTTCTCTTCTGCTGGGCCTTTGAACAAGA
  AGAGAGACCTACCTTCACCAAGCTCATGGACATGCTGGAGAAACTGCCAAAGCGAAACCGTCGCCTGTCT
  CACCCTGGACATTTCTGGAAGTCTGCAGAGCTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC211665 representing NM_173598
  Red=Cloning site Green=Tags(s)
  
  MIDLSISNLEGLRTKCATSNDLTQKEIRTLESKLVKYFSRQLSCKKKVALQERNAELDGFPQLRHWFRIV
  DVRKEVLEEISPGQLSLEDLLEMTDEQVCETVEKYGANREECARLNASLSCLRNVHMSGGNLSKQDWTIQ
  WPTTETGKENNPVCPPEPTPWIRTHLSQSPRVPSKCVQHYCHTSPTPGAPVYTHVDRLTVDAYPGLCPPP
  PLESGHRSLPPSPRQRHAVRTPPRTPNIVTTVTPPGTPPMRKKNKLKPPGTPPPSSRKLIHLIPGFTALH
  RSKSHEFQLGHRVDEAHTPKAKKKSKPLNLKIHSSVGSCENIPSQQRSPLLSERSLRSFFVGHAPFLPST
  PPVHTEANFSANTLSVPRWSPQIPRRDLGNSIKHRFSTKYWMSQTCTVCGKGMLFGLKCKNCKLKCHNKC
  TKEAPPCHLLIIHRGDPARLVRTESVPCDINNPLRKPPRYSDLHISQTLPKTNKINKDHIPVPYQPDSSS
  NPSSTTSSTPSSPAPPLPPSATPPSPLHPSPQCTRQQKNFNLPASHYYKYKQQFIFPDVVPVPETPTRAP
  QVILHPVTSNPILEGNPLLQIEVEPTSENEEVHDEAEESEDDFEEMNLSLLSARSFPRKASQTSIFLQEW
  DIPFEQLEIGELIGKGRFGQVYHGRWHGEVAIRLIDIERDNEDQLKAFKREVMAYRQTRHENVVLFMGAC
  MSPPHLAIITSLCKGRTLYSVVRDAKIVLDVNKTRQIAQEIVKGMGYLHAKGILHKDLKSKNVFYDNGKV
  VITDFGLFSISGVLQAGRREDKLRIQNGWLCHLAPEIIRQLSPDTEEDKLPFSKHSDVFALGTIWYELHA
  REWPFKTQPAEAIIWQMGTGMKPNLSQIGMGKEISDILLFCWAFEQEERPTFTKLMDMLEKLPKRNRRLS
  HPGHFWKSAEL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_173598
• ORF Size: 2763 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_173598.4, NP_775869.3
• RefSeq Size: 17008 bp
• RefSeq ORF: 2853 bp
• Locus ID: 283455
• UniProt ID: Q6VAB6
• Cytogenetics: 12q24.22-q24.23
• Protein Families: Druggable Genome, Protein Kinase
• MW: 104.1 kDa
• Gene Summary: Location-regulated scaffold connecting MEK to RAF. Has very low protein kinase activity and can phosphorylate MAP2K1 at several Ser and Thr residues with very low efficiency (in vitro). Interaction with BRAF enhances KSR2-mediated phosphorylation of MAP2K1 (in vitro). Blocks MAP3K8 kinase activity and MAP3K8-mediated signaling. Acts as a negative regulator of MAP3K3-mediated activation of ERK, JNK and NF-kappa-B pathways, inhibiting MAP3K3-mediated interleukin-8 production.[UniProtKB/Swiss-Prot Function]