WDFY2 (NM_052950) Human Tagged ORF Clone

CAT#: RC204225

WDFY2 (Myc-DDK-tagged)-Human WD repeat and FYVE domain containing 2 (WDFY2)

Product Images

Circular map for RC204225

• 

  Coomassie blue staining of purified WDFY2 protein (Cat# TP304225). The protein was produced from HEK293T cells transfected with WDFY2 cDNA clone (Cat# RC204225) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: WDFY2
• Synonyms: PROF; WDF2; ZFYVE22
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC204225 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCGGAGATCCAGCCCAAGCCTCTGACCCGCAAGCCGATCCTGCTGCAGCGGATGGAGGGGTCCC
  AGGAGGTGGTGAATATGGCCGTGATCGTGCCCAAAGAGGAGGGCGTCATCAGCGTCTCCGAGGACAGGAC
  AGTTCGTGTTTGGTTAAAGAGAGACAGTGGACAGTATTGGCCAAGCGTATACCATGCAATGCCTTCTCCA
  TGTTCATGCATGTCTTTTAACCCGGAAACAAGAAGACTGTCCATAGGTCTAGACAATGGTACAATCTCAG
  AGTTTATATTGTCAGAAGATTATAACAAGATGACTCCTGTGAAAAACTATCAAGCGCATCAGAGCAGAGT
  GACGATGATCCTGTTTGTCCTGGAGCTGGAGTGGGTGCTGAGCACAGGACAGGACAAGCAATTTGCCTGG
  CACTGCTCTGAGAGTGGGCAGCGCCTGGGAGGTTATCGGACCAGTGCTGTGGCCTCAGGCCTGCAATTTG
  ATGTTGAAACCCGGCATGTGTTTATCGGTGACCACTCAGGCCAAGTAACAATCCTCAAACTGGAGCAAGA
  AAACTGCACCCTGGTCACAACATTCAGAGGACACACAGGTGGGGTGACCGCTCTCTGTTGGGACCCAGTC
  CAGCGGGTGTTGTTCTCAGGCAGTTCAGATCACTCTGTCATCATGTGGGACATCGGTGGGAGAAAAGGAA
  CAGCCATCGAGCTCCAAGGACACAACGACAGAGTCCAGGCCCTCTCCTATGCACAGCACACGCGACAATT
  GATCTCCTGTGGCGGTGATGGTGGGATTGTCGTCTGGAACATGGACGTGGAGAGGCAGGAGACCCCTGAA
  TGGTTGGACAGTGATTCCTGCCAAAAGTGTGATCAGCCTTTCTTCTGGAACTTCAAGCAAATGTGGGACA
  GTAAGAAAATTGGTCTAAGACAGCACCACTGCCGCAAGTGTGGGAAGGCCGTCTGTGGCAAGTGCAGCTC
  CAAGCGCTCCTCCATCCCCCTGATGGGCTTCGAGTTTGAAGTGAGGGTCTGTGACAGCTGCCACGAGGCC
  ATCACAGATGAAGAACGTGCACCCACAGCCACCTTCCATGACAGTAAACATAACATTGTGCATGTGCATT
  TCGATGCAACCAGAGGATGGTTACTGACTTCTGGAACTGACAAGGTTATTAAGTTGTGGGATATGACCCC
  AGTCGTGTCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC204225 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAAEIQPKPLTRKPILLQRMEGSQEVVNMAVIVPKEEGVISVSEDRTVRVWLKRDSGQYWPSVYHAMPSP
  CSCMSFNPETRRLSIGLDNGTISEFILSEDYNKMTPVKNYQAHQSRVTMILFVLELEWVLSTGQDKQFAW
  HCSESGQRLGGYRTSAVASGLQFDVETRHVFIGDHSGQVTILKLEQENCTLVTTFRGHTGGVTALCWDPV
  QRVLFSGSSDHSVIMWDIGGRKGTAIELQGHNDRVQALSYAQHTRQLISCGGDGGIVVWNMDVERQETPE
  WLDSDSCQKCDQPFFWNFKQMWDSKKIGLRQHHCRKCGKAVCGKCSSKRSSIPLMGFEFEVRVCDSCHEA
  ITDEERAPTATFHDSKHNIVHVHFDATRGWLLTSGTDKVIKLWDMTPVVS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_052950
• ORF Size: 1200 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_052950.4
• RefSeq Size: 3809 bp
• RefSeq ORF: 1203 bp
• Locus ID: 115825
• UniProt ID: Q96P53
• Cytogenetics: 13q14.3
• Domains: FYVE, WD40
• MW: 45.2 kDa
• Gene Summary: This gene encodes a protein that contains two WD domains and an FYVE zinc finger region. The function of this gene is unknown. An alternatively spliced transcript variant of this gene may exist. [provided by RefSeq, Jul 2008]