NAP1L4 (NM_005969) Human Tagged ORF Clone

CAT#: RC201407

NAP1L4 (Myc-DDK-tagged)-Human nucleosome assembly protein 1-like 4 (NAP1L4)

Product Images

Circular map for RC201407

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  Western blot validation of overexpression lysate (Cat# LY416953) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC201407 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified NAP1L4 protein (Cat# TP301407). The protein was produced from HEK293T cells transfected with NAP1L4 cDNA clone (Cat# RC201407) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: NAP1L4
• Synonyms: hNAP2; NAP1L4b; NAP2; NAP2L
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC201407 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGATCACAGTTTTTCAGATGGGGTTCCTTCAGATTCCGTGGAAGCTGCTAAAAATGCAAGTAACA
  CAGAAAAGCTCACAGATCAGGTGATGCAGAATCCTCGAGTTCTGGCAGCTTTACAGGAGCGACTTGACAA
  TGTCCCTCACACCCCTTCCAGCTACATCGAAACTTTACCTAAAGCAGTAAAAAGAAGAATTAATGCATTG
  AAACAACTTCAGGTGAGATGTGCTCACATAGAAGCCAAGTTCTATGAAGAGGTACATGACTTGGAAAGAA
  AGTATGCAGCGCTATACCAGCCTCTCTTTGACAAGAGAAGAGAATTTATCACCGGCGATGTTGAACCAAC
  AGATGCGGAATCGGAATGGCACAGTGAAAATGAAGAGGAAGAGAAATTGGCTGGAGACATGAAAAGTAAA
  GTAGTCGTCACAGAAAAAGCAGCGGCAACGGCTGAAGAGCCAGATCCCAAAGGAATTCCAGAGTTCTGGT
  TTACCATCTTCAGAAATGTGGACATGCTGAGTGAATTAGTCCAGGAATATGATGAACCAATCTTGAAACA
  CCTGCAGGATATTAAAGTGAAATTTTCTGACCCTGGACAGCCTATGTCTTTTGTGTTAGAGTTCCACTTT
  GAACCCAACGACTACTTTACCAACTCAGTCCTGACAAAAACCTACAAGATGAAATCAGAACCAGATAAGG
  CTGATCCCTTTTCCTTTGAAGGTCCTGAGATTGTGGACTGTGACGGGTGTACTATTGACTGGAAGAAAGG
  AAAGAATGTTACTGTCAAAACCATCAAGAAAAAGCAGAAGCATAAGGGTCGAGGCACTGTTAGAACAATT
  ACGAAACAAGTACCCAATGAGTCCTTTTTCAACTTCTTCAATCCATTGAAAGCATCCGGGGATGGAGAAT
  CACTGGATGAAGATTCTGAATTCACATTAGCCTCTGATTTTGAAATTGGACACTTTTTCCGTGAGCGGAT
  AGTCCCGCGGGCTGTGCTGTACTTCACTGGGGAGGCCATAGAAGATGATGACAATTTTGAAGAAGGTGAA
  GAAGGAGAAGAGGAGGAATTAGAAGGTGACGAGGAGGGAGAAGACGAGGATGATGCGGAAATTAACCCCA
  AGGTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC201407 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MADHSFSDGVPSDSVEAAKNASNTEKLTDQVMQNPRVLAALQERLDNVPHTPSSYIETLPKAVKRRINAL
  KQLQVRCAHIEAKFYEEVHDLERKYAALYQPLFDKRREFITGDVEPTDAESEWHSENEEEEKLAGDMKSK
  VVVTEKAAATAEEPDPKGIPEFWFTIFRNVDMLSELVQEYDEPILKHLQDIKVKFSDPGQPMSFVLEFHF
  EPNDYFTNSVLTKTYKMKSEPDKADPFSFEGPEIVDCDGCTIDWKKGKNVTVKTIKKKQKHKGRGTVRTI
  TKQVPNESFFNFFNPLKASGDGESLDEDSEFTLASDFEIGHFFRERIVPRAVLYFTGEAIEDDDNFEEGE
  EGEEEELEGDEEGEDEDDAEINPKV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_005969
• ORF Size: 1125 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005969.4
• RefSeq Size: 2564 bp
• RefSeq ORF: 1128 bp
• Locus ID: 4676
• UniProt ID: Q99733
• Cytogenetics: 11p15.4
• Domains: NAP
• MW: 42.8 kDa
• Gene Summary: This gene encodes a member of the nucleosome assembly protein (NAP) family which can interact with both core and linker histones. It can shuttle between the cytoplasm and nucleus, suggesting a role as a histone chaperone. This gene is one of several located near the imprinted gene domain of 11p15.5, an important tumor-suppressor gene region. Alterations in this region have been associated with the Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocortical carcinoma, and lung, ovarian, and breast cancer. [provided by RefSeq, Jul 2008]