Nfat5 (NM_001286260) Mouse Tagged ORF Clone

CAT#: MR231698

Nfat5 (myc-DDK-tagged) - Mouse nuclear factor of activated T cells 5 (Nfat5), transcript variant c

Product Images

Circular map for MR231698

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Nfat5
• Synonyms: AI225870; B130038B15Rik; CAG-8; CAG80; mKIAA0827; NFATL1; nfatz; OREBP; TonEBP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR231698 representing NM_001286260
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCCTCGGACTTCATCTCATTGCTCAGCGCGGACCTAGACCTGGAATCGCCCAAGTCCCTGTACTCGC
  GAGAATCTGTCTATGATCTTCTCCCAAAGGAGTTACAGTTACCTCCACCTAGAGAAACATCTGTAGCATC
  AATGAGTCAGACAAGCGGTGGTGAGGCAGGCTCGCCTCCTCCAGCTGTAGTTGCTGCTGATGCTTCTTCA
  GCTCCCTCCTCTTCCTCCATGGGCGGTGCTTGCAGCTCCTTTACCACCTCTTCCAGCCCTACCATTTATT
  CTACCTCAGTCACCGACAGCAAGGCTATGCAAGTGGAGAGCTGCTCCTCAGCCGTGGGGGTAAGTAACAG
  AGGGGTAAGTGAAAAGCAGTTAACCGGTAACACAGTTCAGCAGCATCCATCAACCCCGAAGAGGCACACA
  GTTTTGTACATCTCACCACCACCTGAGGACTTGCTGGATAACAGTCGGATGTCCTGCCAGGATGAGGGGT
  GTGGATTGGAATCTGAGCAGAGCTGCAGTATGTGGATGGAGGATTCCCCCTCCAACTTCAGTAACATGAG
  CACCAGTTCCTACAATGATAACACTGAGGTACCTCGTAAATCACGAAAACGAAATCCAAAGCAGAGGCCG
  GGGGTCAAACGACGAGATTGTGAAGAATCTAATATGGATATATTTGATGCCGACAGTGCCAAAGCACCTC
  ACTATGTGCTTTCTCAGCTTACCACGGACAACAAAGGCAACTCAAAAGCTGGAAATGGAACATTGGACAG
  CCAAAAGGGAACTGGAGTAAAGAAGAGCCCTATGTTGTGCGGACAGTATCCGGTTAAAAGTGAGGGGAAG
  GAGCTGAAGATAGTGGTACAGCCTGAAACCCAACACCGAGCCCGGTACCTGACAGAGGGCAGCCGAGGCT
  CTGTAAAAGACAGAACACAGCAAGGCTTTCCTACGGTGAAGCTGGAAGGCCATAATGAACCAGTGGTGTT
  GCAGGTATTTGTGGGCAATGATTCTGGTCGAGTAAAACCACATGGATTCTATCAGGCCTGTAGAGTAACT
  GGGCGAAATACAACTCCCTGCAAAGAAGTGGACATTGAAGGTACCACTGTTATAGAAGTTGGTCTTGATC
  CTAGCAACAACATGACACTGGCGGTGGACTGTGTTGGAATATTGAAGCTGAGGAATGCCGATGTTGAAGC
  CAGAATTGGAATTGCTGGATCTAAGAAAAAGAGCACTCGTGCCAGATTGGTTTTTCGAGTTAATATCACA
  AGGAAAGATGGCTCTACTCTGACATTGCAGACACCTTCTTCCCCCATTTTATGCACTCAGCCAGCAGGAG
  TTCCTGAGATCTTAAAGAAAAGCTTGCATAGCTGTTCAGTGAAAGGAGAGGAAGAAGTATTTTTAATTGG
  GAAAAACTTTCTGAAAGGAACTAAAGTTATTTTCCAGGAAAATGTTTCTGATGAAAACTCTTGGAAATCA
  GAAGCTGAAATTGACATGGAGTTATTCCATCAGAACCATCTTATTGTGAAGGTTCCTCCGTATCATGACC
  AACATATAACTTTGCCTGTATCAGTGGGAATATATGTTGTGACCAATGCTGGAAGATCTCATGATGTTCA
  GCCATTTACGTACACTCCGGATCCAGCAGCTGGTGCTTTGAATGTAAATGTGAAAAAGGAGATATCTAGT
  CCAGCAAGACCTTGCTCTTTTGAAGAAGCCATGAAAGCAATGAAAACGACTGGATGTAACGTAGATAAGG
  TGACTATCCTTCCTAATGCCCTGATCACTCCACTCATATCAAGCAGTATGATTAAGACTGAAGATGTTAC
  TCCAATGGAAGTAACTTCAGAAAAAAGATCTTCCCCAATTTTTCAGACTACAAAGAGCATTGGATCAACC
  CAGCAAACCTTAGAAACTATTTCTAACATAGCGGGGGGTGCACCCTTCTCCTCACCGTCGTCATCTTCCC
  ACTTAACTCCTGAAAGTGAGAATCAGCAGCAGCTTCAGCCCAAGGCATACAACCCAGAGACCCTGACAAC
  TATCCAGACACAGGACATCTCACAGCCCGGGACCTTTCCCGCAGTTTCTGCTGCTAGTCAGCTGCCCAGC
  AGCGATGCACTACTGCAGCAGGCAACACAGTTTCAGACAAGAGAAGCTCAGTCCAGAGACACAATACAGT
  CAGATACAGTGGTTAACTTGTCACAGTTAACTGAAGCATCACAGCAGCAGCAATCCCCACTACAAGAACA
  AGCACAAACGTTACAGCAGCAGATACCATCAAATATTTTTCCATCGCCAAGCAGTGTCAGCCAGCTCCAG
  AGTACTATTCAGCAGCTGCAAGCAGGAAGTTTCACAGGCAGCGCTGCTGGCGGCAGGAGTGGAAGCGTTG
  ATTTGGTCCAGCAGGTTTTAGAGGCACAACAGCAGTTATCGTCTGTTCTGTTTTCTACTCCAGATGGGAA
  TGAGAATGTTCAAGAACAGCTTAATGCAGACATTTTCCAAGTCAGTCAAATTCAAAATAGTGTAAGCCCT
  GGAATGTTTTCTTCAGCGGAGTCTGCAGTTCACACTAGACCAGATAACTTACTACCTGGGAGGGCTGACA
  GCGTCCATCAACAGACTGAAAATACACTGTCTAATCAGCAGCAGCAGCAACAGCAGCAGCAGCAAGTGAT
  GGAGTCATCAGCTGCAATGGTGATGGAGATGCAGCAGAGCATTTGCCAAGCAGCTGCCCAGATCCAGTCA
  GAGCTGTTTCCTTCAGCTGCTTCAGCAAGTGGAAGCCTTCAGCAGTCTCCAGTTTACCAGCAGCCTTCTC
  ACATGATGAGTGCACTGCCTACCAACGAGGACATGCAGATGCAGTGTGAGCTGTTCTCGTCTCCCCCTGC
  AGCTTCTGGAAACGAAACTTCCACAACCACCACGCCACAGGTGGCAACCCCTGGCTCCACCATGTTTCAG
  ACACCAAGTTCAGGAGATGGAGAAGAAACTGGAGCACAAGCAAAACAGATTCAGAACAGCGTCTTTCAGA
  CAATGGTCCAAATGCAGCGCAGTGGTGACAGCCAACCTCAAGTAAACCTCTTTTCATCTACAAAAAATAT
  AATGAGTGTTCAGAATAATGGTACCCAGCAACAAGGGAATAGCTTGTTCCAGCAAGGGAGCGAGATGATG
  TCACTTCAGTCTGGGAACTTTTTGCAGCAGTCCTCTCATTCACAGGCTCAGCTCTTTCATCCTCAAAATC
  CTATTGCTGATGCTCAGAACCTTTCCCAGGAAACTCAAGGTTCAATTTTTCATAGTCCAAATCCTATTGT
  CCACAGTCAAACTTCCACAGCATCCTCTGAACAACTGCAGCCTTCAATGTTTCACTCTCAGAATACCATT
  GCTGTGTTACAGGGCTCTTCAGTTCCTCAAGACCAGCAGTCACCCAACATATTTCTTTCCCAAAGTTCTA
  TCAATAATCTTCAAACTAACACAGTAGCCCAAGAAGAGCAGATTTCATTTTTTGCAGCACAGAACTCAAT
  TTCTCCACTTCAGTCAACATCAAACACTGAGCAGCAAGCTGCTTTCCAACAGCAGCCTCCAATCTCACAC
  ATCCAGACCCCTATTCTTTCCCAGGAACAGGCACAACCCTCTCAGCAAGGGTTATTTCAGCCTCAGGAAA
  GCTTACACTCACACATAACCCCAGACGCTTGCAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR231698 representing NM_001286260
  Red=Cloning site Green=Tags(s)
  
  MPSDFISLLSADLDLESPKSLYSRESVYDLLPKELQLPPPRETSVASMSQTSGGEAGSPPPAVVAADASS
  APSSSSMGGACSSFTTSSSPTIYSTSVTDSKAMQVESCSSAVGVSNRGVSEKQLTGNTVQQHPSTPKRHT
  VLYISPPPEDLLDNSRMSCQDEGCGLESEQSCSMWMEDSPSNFSNMSTSSYNDNTEVPRKSRKRNPKQRP
  GVKRRDCEESNMDIFDADSAKAPHYVLSQLTTDNKGNSKAGNGTLDSQKGTGVKKSPMLCGQYPVKSEGK
  ELKIVVQPETQHRARYLTEGSRGSVKDRTQQGFPTVKLEGHNEPVVLQVFVGNDSGRVKPHGFYQACRVT
  GRNTTPCKEVDIEGTTVIEVGLDPSNNMTLAVDCVGILKLRNADVEARIGIAGSKKKSTRARLVFRVNIT
  RKDGSTLTLQTPSSPILCTQPAGVPEILKKSLHSCSVKGEEEVFLIGKNFLKGTKVIFQENVSDENSWKS
  EAEIDMELFHQNHLIVKVPPYHDQHITLPVSVGIYVVTNAGRSHDVQPFTYTPDPAAGALNVNVKKEISS
  PARPCSFEEAMKAMKTTGCNVDKVTILPNALITPLISSSMIKTEDVTPMEVTSEKRSSPIFQTTKSIGST
  QQTLETISNIAGGAPFSSPSSSSHLTPESENQQQLQPKAYNPETLTTIQTQDISQPGTFPAVSAASQLPS
  SDALLQQATQFQTREAQSRDTIQSDTVVNLSQLTEASQQQQSPLQEQAQTLQQQIPSNIFPSPSSVSQLQ
  STIQQLQAGSFTGSAAGGRSGSVDLVQQVLEAQQQLSSVLFSTPDGNENVQEQLNADIFQVSQIQNSVSP
  GMFSSAESAVHTRPDNLLPGRADSVHQQTENTLSNQQQQQQQQQQVMESSAAMVMEMQQSICQAAAQIQS
  ELFPSAASASGSLQQSPVYQQPSHMMSALPTNEDMQMQCELFSSPPAASGNETSTTTTPQVATPGSTMFQ
  TPSSGDGEETGAQAKQIQNSVFQTMVQMQRSGDSQPQVNLFSSTKNIMSVQNNGTQQQGNSLFQQGSEMM
  SLQSGNFLQQSSHSQAQLFHPQNPIADAQNLSQETQGSIFHSPNPIVHSQTSTASSEQLQPSMFHSQNTI
  AVLQGSSVPQDQQSPNIFLSQSSINNLQTNTVAQEEQISFFAAQNSISPLQSTSNTEQQAAFQQQPPISH
  IQTPILSQEQAQPSQQGLFQPQESLHSHITPDACK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001286260
• ORF Size: 3675 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001286260.1, NP_001273189.1
• RefSeq Size: 4725 bp
• RefSeq ORF: 3678 bp
• Locus ID: 54446
• UniProt ID: Q9WV30
• Cytogenetics: 8 53.93 cM
• MW: 132.6 kDa
• Gene Summary: Transcription factor involved in the transcriptional regulation of osmoprotective and inflammatory genes. Regulates hypertonicity-induced cellular accumulation of osmolytes.[UniProtKB/Swiss-Prot Function]