Chfr (NM_001289580) Mouse Tagged ORF Clone

CAT#: MR230363

Chfr (myc-DDK-tagged) - Mouse checkpoint with forkhead and ring finger domains (Chfr), transcript variant 5

Product Images

Circular map for MR230363

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Chfr
• Synonyms: 5730484M20Rik; C230082M18; RNF116
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR230363 representing NM_001289580
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTCCATGTGACCAAAGATTGCTCAGGTCCAGGGCAGGGTGATGATCCCCAGGTTCCACTATTGTCAC
  CCATGGCTCAGACATGCTTAGAGGAACCACAGCCATCAACATCGACATCAGACCTCCTCCCCACGGCCTC
  TACCTCTTCTACGGAGCCAGAGCTGACCTCTGCAGGGCAAAAGCATTCTTCTAGCTCTGGACCTGGGAAC
  ACAAGCATCTCCCCAAAAGGACGCAGTTCACTTGTTGCAAATGGCGAACTCTCTAGCCTTTCTCCAGTTT
  TCCAAGACAAAGAAGCATCCTTTTCTTTGCTGGAAAGTAAAGACCATGAGGAATTGGAGCCTGCCAAAAA
  AAAGATGAAAGGAGATGGGGAACTTGACACGAACCTCCAGTTATTAGTTTCAGGCCAGCGTGGAAATGCC
  CAAACCTCAAGTGAAGATGTCAAAGATGCCTCTGTGAAGCCAGACAAGATGGAGGAGACACTAACCTGTA
  TCATCTGCCAGGACCTTCTGCACGATTGTGTGAGTTTGCAGCCTTGTATGCACACATTTTGTGCGGCTTG
  CTACTCTGGTTGGATGGAGCGTTCATCTCTGTGCCCTACCTGCCGATGTCCAGTGGAGCGGATTTGCAAA
  AACCACATCCTGAACAACCTAGTGGAAGCATACCTTATCCAGCACCCAGATAAAAGTCGCAGTGAAGAAG
  ATGTGAGAAGTATGGATGCAAGGAATAAAATCACTCAAGATATGCTGCAACCCAAAGTCAGGAGGTCTTT
  CTCTGATGAAGAGGGGAGTTCAGAGGACCTGCTAGAGCTGTCTGATGTCGACAGTGAATCCTCAGATATC
  AGTCAGCCATACATTGTCTGCAGACAGTGTCCTGAATACAGAAGGCAAGCGGTGCAGTCTCTTCCTTGCC
  CAGTCCCAGAGAGTGAGCTGGGAGCTACACTGGCCCTTGGTGGGGAGGCACCTTCAACATCTGCCAGCTT
  GCCAACAGCCCCGGATTACATGTGCCCTCTTCAAGGAAGCCATGCCATATGCACCTGCTGCTTCCAGCCT
  ATGCCTGACCGGAGAGCTGAACGGGAGCAGGATCCCCGCGTCGCCCCTCAGCAGTGTGCGGTGTGCCTGC
  AGCCCTTCTGCCACCTGTACTGGGGCTGCACGAGGACTGGCTGCTTTGGCTGCTTGGCCCCATTCTGTGA
  GCTCAACCTGGGGGACAAGTGCTTGGATGGAGTGCTGAACAATAACAATTATGAATCGGACATCCTGAAG
  AATTACCTGGCAACCAGGGGTCTGACATGGAAAAGTGTGTTGACAGAGAGTCTCCTGGCTCTGCAGCGAG
  GTGTATTTATGCTGTCTGATTACAGAATCACTGGAAATACTGTGCTGTGTTACTGCTGTGGTCTGCGTAG
  CTTCCGAGAGCTGACCTACCAGTATCGTCAGAACATTCCTGCTTCTGAGTTGCCAGTGACTGTAACATCC
  CGTCCTGACTGCTACTGGGGCCGTAACTGTCGCACTCAGGTGAAGGCTCACCATGCAATGAAATTCAATC
  ACATCTGTGAGCAAACAAGGTTCAAGAAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR230363 representing NM_001289580
  Red=Cloning site Green=Tags(s)
  
  MFHVTKDCSGPGQGDDPQVPLLSPMAQTCLEEPQPSTSTSDLLPTASTSSTEPELTSAGQKHSSSSGPGN
  TSISPKGRSSLVANGELSSLSPVFQDKEASFSLLESKDHEELEPAKKKMKGDGELDTNLQLLVSGQRGNA
  QTSSEDVKDASVKPDKMEETLTCIICQDLLHDCVSLQPCMHTFCAACYSGWMERSSLCPTCRCPVERICK
  NHILNNLVEAYLIQHPDKSRSEEDVRSMDARNKITQDMLQPKVRRSFSDEEGSSEDLLELSDVDSESSDI
  SQPYIVCRQCPEYRRQAVQSLPCPVPESELGATLALGGEAPSTSASLPTAPDYMCPLQGSHAICTCCFQP
  MPDRRAEREQDPRVAPQQCAVCLQPFCHLYWGCTRTGCFGCLAPFCELNLGDKCLDGVLNNNNYESDILK
  NYLATRGLTWKSVLTESLLALQRGVFMLSDYRITGNTVLCYCCGLRSFRELTYQYRQNIPASELPVTVTS
  RPDCYWGRNCRTQVKAHHAMKFNHICEQTRFKN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001289580
• ORF Size: 1569 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001289580.1, NP_001276509.1
• RefSeq Size: 3347 bp
• RefSeq ORF: 1572 bp
• Locus ID: 231600
• UniProt ID: Q810L3
• Cytogenetics: 5 F
• MW: 58.3 kDa
• Gene Summary: E3 ubiquitin-protein ligase that functions in the antephase checkpoint by actively delaying passage into mitosis in response to microtubule poisons. Acts in early prophase before chromosome condensation, when the centrosome move apart from each other along the periphery of the nucleus. Probably involved in signaling the presence of mitotic stress caused by microtubule poisons by mediating the 'Lys-48'-linked ubiquitination of target proteins, leading to their degradation by the proteasome. Promotes the ubiquitination and subsequent degradation of AURKA and PLK1. Probably acts as a tumor suppressor, possibly by mediating the polyubiquitination of HDAC1, leading to its degradation. May also promote the formation of 'Lys-63'-linked polyubiquitin chains and functions with the specific ubiquitin-conjugating UBC13-MMS2 (UBE2N-UBE2V2) heterodimer. Substrates that are polyubiquitinated at 'Lys-63' are usually not targeted for degradation, but are rather involved in signaling cellular stress (By similarity).[UniProtKB/Swiss-Prot Function]