Tlr1 (NM_030682) Mouse Tagged ORF Clone

CAT#: MR226857

Tlr1 (Myc-DDK-tagged) - Mouse toll-like receptor 1 (Tlr1)

Product Images

Circular map for MR226857

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Tlr1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR226857 representing NM_030682
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACTAAACCAAATTCCCTCATCTTCTACTGTATCATTGTTTTAGGACTGACACTTATGAAAATCCAAT
  TATCTGAGGAATGTGAGCTTATCATAAAGAGGCCAAACGCAAACCTTACCAGAGTGCCCAAGGACCTACC
  CTTGCAAACAACTACTTTAGATCTATCACAAAACAATATATCTGAGCTTCAGACTTCTGACATCCTCTCA
  TTGTCCAAGCTGAGGGTCCTGATAATGTCCTACAACAGACTCCAGTATCTTAATATCAGTGTTTTCAAAT
  TCAACACAGAGCTGGAATATTTGGATTTGTCCCACAATGAGCTAAAGGTGATCTTGTGCCACCCAACAGT
  CAGCCTCAAGCATTTGGACCTCTCCTTTAATGCCTTTGATGCCCTGCCTATATGCAAAGAATTTGGCAAC
  ATGTCCCAACTACAGTTCCTGGGGTTGAGCGGTTCTCGGGTACAAAGTTCAAGTGTGCAGCTGATTGCTC
  ATTTGAACATCAGTAAGGTTTTGCTGGTGTTAGGAGATGCTTATGGGGAAAAAGAAGACCCCGAATCTCT
  TCGGCACGTTAGCACTGAGACTCTGCATATTGTTTTCCCGTCGAAAAGAGAATTCCGTTTTCTTCTGGAT
  GTGTCCGTCAGCACTACGATCGGTTTGGAACTGTCTAACATCAAGTGTGTGCTTGAAGACCAGGGCTGCT
  CTTATTTCTTACGTGCTTTGTCAAAGCTTGGAAAGAATCTGAAGCTCTCAAATCTTACCCTGAACAATGT
  GGAAACAACGTGGAATTCCTTCATTAATATCCTCCAGATAGTTTGGCATACGCCAGTCAAATATTTCTCA
  ATTTCAAATGTGAAGCTACAAGGTCAACTTGCCTTCAGGATGTTCAATTATTCTGACACTTCTCTGAAGG
  CTTTGTCGATACATCAAGTTGTCACTGATGTCTTCAGCTTCCCCCAAAGTTACATATACAGTATCTTTGC
  CAATATGAACATCCAAAACTTTACAATGTCTGGAACACACATGGTCCACATGCTGTGCCCGTCCCAAGTT
  AGCCCATTTCTGCATGTGGACTTTACAGATAACCTTTTAACAGACATGGTTTTTAAAGACTGTAGAAACT
  TAGTTAGATTGAAAACACTTAGTTTACAAAAGAATCAGTTAAAAAACCTTGAGAATATAATCCTCACATC
  TGCAAAGATGACATCCCTACAAAAACTAGACATTAGCCAGAATTCTCTAAGGTACAGCGATGGGGGAATC
  CCATGCGCCTGGACCCAGAGTTTGTTAGTTTTAAATTTGTCTTCGAATATGCTTACAGGCTCTGTCTTCA
  GATGCTTACCTCCCAAAGTCAAGGTCCTTGACCTTCACAACAACAGGATAATGAGCATCCCTAAAGATGT
  CACCCACCTGCAGGCTTTGCAGGAACTCAATGTAGCATCCAACTCCTTAACTGACCTTCCTGGGTGCGGG
  GCCTTCAGCAGCCTTTCTGTGCTGGTCATCGACCATAACTCAGTTTCCCATCCCTCTGAGGATTTCTTCC
  AGAGCTGTCAGAATATTAGATCCCTAACAGCGGGAAACAACCCATTCCAATGCACATGTGAGCTGAGGGA
  CTTTGTCAAGAACATAGGCTGGGTAGCAAGAGAAGTGGTGGAGGGCTGGCCTGACTCTTACAGGTGTGAC
  TACCCAGAAAGCTCTAGGGGAACTGCACTGAGGGACTTCCACATGTCTCCACTATCCTGTGATACTGTTC
  TGCTGACTGTCACCATCGGGGCCACTATGCTGGTGCTGGCTGTCACTGGGGCTTTCCTCTGTCTCTACTT
  TGACCTGCCCTGGTATGTGAGGATGCTGTGTCAGTGGACACAGACCAGGCACAGGGCCAGGCACATCCCC
  TTAGAGGAACTCCAGAGAAACCTCCAGTTCCATGCTTTTGTCTCATACAGTGGGCATGATTCTGCCTGGG
  TGAAGAACGAATTACTACCCAACCTAGAGAAAGATGACATCCAGATTTGCCTCCATGAGAGGAACTTTGT
  CCCTGGCAAGAGCATTGTGGAGAACATCATCAATTTCATTGAGAAGAGTTACAAGTCCATCTTTGTGCTG
  TCTCCCCACTTCATCCAGAGTGAGTGGTGTCATTATGAACTCTATTTTGCCCATCACAATCTCTTCCATG
  AAGGCTCTGATAACTTAATCCTCATCTTGCTGGCACCCATTCCCCAGTACTCCATCCCTACCAATTACCA
  CAAGCTCAAAACTCTCATGTCACGAAGGACCTATCTGGAATGGCCCACAGAGAAGAACAAGCATGGACTT
  TTTTGGGCAAACCTAAGAGCATCCATTAATGTTAAGCTGGTTAACCAGGCAGAAGGAACGTGTTACACAC
  AGCAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR226857 representing NM_030682
  Red=Cloning site Green=Tags(s)
  
  MTKPNSLIFYCIIVLGLTLMKIQLSEECELIIKRPNANLTRVPKDLPLQTTTLDLSQNNISELQTSDILS
  LSKLRVLIMSYNRLQYLNISVFKFNTELEYLDLSHNELKVILCHPTVSLKHLDLSFNAFDALPICKEFGN
  MSQLQFLGLSGSRVQSSSVQLIAHLNISKVLLVLGDAYGEKEDPESLRHVSTETLHIVFPSKREFRFLLD
  VSVSTTIGLELSNIKCVLEDQGCSYFLRALSKLGKNLKLSNLTLNNVETTWNSFINILQIVWHTPVKYFS
  ISNVKLQGQLAFRMFNYSDTSLKALSIHQVVTDVFSFPQSYIYSIFANMNIQNFTMSGTHMVHMLCPSQV
  SPFLHVDFTDNLLTDMVFKDCRNLVRLKTLSLQKNQLKNLENIILTSAKMTSLQKLDISQNSLRYSDGGI
  PCAWTQSLLVLNLSSNMLTGSVFRCLPPKVKVLDLHNNRIMSIPKDVTHLQALQELNVASNSLTDLPGCG
  AFSSLSVLVIDHNSVSHPSEDFFQSCQNIRSLTAGNNPFQCTCELRDFVKNIGWVAREVVEGWPDSYRCD
  YPESSRGTALRDFHMSPLSCDTVLLTVTIGATMLVLAVTGAFLCLYFDLPWYVRMLCQWTQTRHRARHIP
  LEELQRNLQFHAFVSYSGHDSAWVKNELLPNLEKDDIQICLHERNFVPGKSIVENIINFIEKSYKSIFVL
  SPHFIQSEWCHYELYFAHHNLFHEGSDNLILILLAPIPQYSIPTNYHKLKTLMSRRTYLEWPTEKNKHGL
  FWANLRASINVKLVNQAEGTCYTQQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_030682
• ORF Size: 2385 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_030682.2, NP_109607.1
• RefSeq Size: 2721 bp
• RefSeq ORF: 2388 bp
• Locus ID: 21897
• UniProt ID: Q9EPQ1
• Cytogenetics: 5 33.53 cM
• MW: 91.1 kDa
• Gene Summary: Participates in the innate immune response to microbial agents. Specifically recognizes diacylated and triacylated lipopeptides. Cooperates with TLR2 to mediate the innate immune response to bacterial lipoproteins or lipopeptides. Forms the activation cluster TLR2:TLR1:CD14 in response to triacylated lipopeptides, this cluster triggers signaling from the cell surface and subsequently is targeted to the Golgi in a lipid-raft dependent pathway. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response (By similarity). Acts as a coreceptor for M.tuberculosis lipoproteins LprG, LpqH and PhoS1 (pstS1), in conjunction with TLR2 and for some but not all lipoproteins CD14 and/or CD36. The lipoproteins act as agonists to modulate antigen presenting cell functions in response to the pathogen (PubMed:19362712).[UniProtKB/Swiss-Prot Function]