Ncam1 (NM_010875) Mouse Tagged ORF Clone

CAT#: MR226649

Ncam1 (Myc-DDK-tagged) - Mouse neural cell adhesion molecule 1 (Ncam1), transcript variant 2

Product Images

Circular map for MR226649

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ncam1
• Synonyms: CD56; E-NCAM; Ncam; NCAM-1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR226649 representing NM_010875
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGCGAACTAAGGATCTCATCTGGACTTTGTTTTTCCTGGGAACTGCAGTTTCCCTGCAGGTAGATA
  TTGTTCCCAGCCAAGGAGAAATCAGCGTTGGAGAGTCCAAATTCTTCCTGTGTCAAGTGGCAGGAGATGC
  CAAAGATAAGGACATCTCCTGGTTCTCCCCCAATGGGGAGAAGCTGAGCCCAAACCAGCAGCGGATCTCA
  GTGGTGTGGAATGATGACGACTCCTCTACCCTCACCATCTACAACGCCAACATCGACGATGCCGGCATAT
  ACAAGTGTGTGGTCACGGCTGAGGACGGCACCCAGTCTGAGGCCACTGTCAACGTGAAGATCTTCCAGAA
  GCTCATGTTCAAGAATGCACCAACCCCACAGGAGTTTAAGGAAGGGGAGGATGCTGTGATTGTCTGTGAT
  GTGGTCAGCTCCCTGCCTCCAACCATCATCTGGAAACACAAAGGCCGAGATGTCATTCTGAAAAAAGACG
  TCCGGTTCATAGTCCTGTCCAACAACTACCTGCAGATCAGGGGCATCAAGAAAACAGATGAGGGTACTTA
  CCGCTGTGAGGGCAGGATCCTGGCCCGCGGGGAAATCAACTTCAAGGACATTCAGGTCATTGTGAATGTA
  CCACCCACTGTCCAGGCCAGACAGAGCATCGTGAATGCCACTGCCAACCTGGGCCAGTCTGTCACCCTGG
  TGTGTGATGCCGATGGCTTCCCAGAGCCCACCATGAGCTGGACAAAGGATGGGGAACCCATAGAGAACGA
  GGAGGAAGATGACGAGAAGCACATCTTCAGTGATGACAGCTCCGAGCTGACCATCAGGAATGTGGATAAA
  AACGACGAGGCCGAATACGTCTGCATCGCAGAGAACAAGGCTGGCGAGCAGGATGCCTCCATCCACCTCA
  AGGTCTTTGCAAAGCCCAAAATCACCTATGTAGAGAATCAGACGGCCATGGAACTAGAGGAGCAAGTCAC
  TCTGACCTGTGAAGCCTCCGGAGACCCCATCCCCTCCATCACCTGGAGAACATCCACCCGGAACATCAGC
  AGTGAAGAAAAGACTCTGGATGGGCACATGGTGGTTCGCAGCCATGCTCGTGTGTCCTCCTTGACCCTGA
  AGAGCATCCAGTACACAGATGCTGGAGAATACATCTGCACTGCCAGCAACACCATCGGCCAGGATTCCCA
  GTCCATGTACCTCGAAGTTCAATATGCTCCCAAGCTCCAGGGCCCTGTGGCTGTGTACACTTGGGAAGGG
  AACCAGGTGAACATCACCTGTGAGGTCTTCGCCTACCCAAGTGCCACAATCTCCTGGTTCCGAGATGGTC
  AGTTGCTGCCAAGCTCCAACTACAGCAATATCAAGATCTACAACACCCCATCTGCTAGCTATCTGGAGGT
  GACCCCTGATTCAGAAAATGACTTTGGAAACTACAACTGTACAGCAGTGAACCGTATTGGACAGGAGTCC
  TTGGAATTCATCCTTGTTCAAGCAGACACACCGTCTTCTCCATCCATTGACCGGGTGGAACCATACTCCA
  GCACAGCGCAGGTGCAGTTTGATGAGCCAGAGGCCACAGGGGGCGTGCCCATTCTCAAGTACAAGGCTGA
  GTGGAAGTCGCTGGGCGAAGAATCCTGGCATTTCAAGTGGTATGATGCCAAAGAAGCCAACATGGAAGGC
  ATTGTCACCATCATGGGCCTGAAACCTGAGACGAGGTACTCGGTACGACTGGCGGCCCTCAACGGCAAGG
  GGCTGGGCGAGATCAGTGCAGCCACTGAGTTCAAGACACAGCCAGTCCGGGAACCCAGTGCACCCAAGCT
  GGAAGGGCAGATGGGAGAGGACGGGAACTCCATCAAGGTGAACCTGATCAAGCAGGATGACGGAGGCTCC
  CCCATCAGACACTATCTGGTCAAGTACAGAGCGCTCGCCTCTGAGTGGAAACCGGAAATCAGGCTCCCAT
  CCGGCAGTGACCACGTCATGCTCAAGTCCCTGGACTGGAACGCAGAGTATGAAGTCTATGTGGTAGCTGA
  AAACCAGCAAGGAAAATCCAAGGCAGCTCACTTTGTGTTCAGGACCTCAGCCCAGCCCACGGCCATCCCA
  GCCACCCTGGGCGGAAGCTCCACCTCCTACACCTTGGTCTCATTGCTTTTCTCTGCGGTGACTCTTCTTC
  TGCTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR226649 representing NM_010875
  Red=Cloning site Green=Tags(s)
  
  MLRTKDLIWTLFFLGTAVSLQVDIVPSQGEISVGESKFFLCQVAGDAKDKDISWFSPNGEKLSPNQQRIS
  VVWNDDDSSTLTIYNANIDDAGIYKCVVTAEDGTQSEATVNVKIFQKLMFKNAPTPQEFKEGEDAVIVCD
  VVSSLPPTIIWKHKGRDVILKKDVRFIVLSNNYLQIRGIKKTDEGTYRCEGRILARGEINFKDIQVIVNV
  PPTVQARQSIVNATANLGQSVTLVCDADGFPEPTMSWTKDGEPIENEEEDDEKHIFSDDSSELTIRNVDK
  NDEAEYVCIAENKAGEQDASIHLKVFAKPKITYVENQTAMELEEQVTLTCEASGDPIPSITWRTSTRNIS
  SEEKTLDGHMVVRSHARVSSLTLKSIQYTDAGEYICTASNTIGQDSQSMYLEVQYAPKLQGPVAVYTWEG
  NQVNITCEVFAYPSATISWFRDGQLLPSSNYSNIKIYNTPSASYLEVTPDSENDFGNYNCTAVNRIGQES
  LEFILVQADTPSSPSIDRVEPYSSTAQVQFDEPEATGGVPILKYKAEWKSLGEESWHFKWYDAKEANMEG
  IVTIMGLKPETRYSVRLAALNGKGLGEISAATEFKTQPVREPSAPKLEGQMGEDGNSIKVNLIKQDDGGS
  PIRHYLVKYRALASEWKPEIRLPSGSDHVMLKSLDWNAEYEVYVVAENQQGKSKAAHFVFRTSAQPTAIP
  ATLGGSSTSYTLVSLLFSAVTLLLL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_010875
• ORF Size: 2175 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_010875.3, NP_035005.2
• RefSeq Size: 4885 bp
• RefSeq ORF: 2178 bp
• Locus ID: 17967
• UniProt ID: P13595
• Cytogenetics: 9 26.83 cM
• MW: 80.8 kDa
• Gene Summary: This protein is a cell adhesion molecule involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc.[UniProtKB/Swiss-Prot Function]