Grik4 (NM_175481) Mouse Tagged ORF Clone

CAT#: MR226146

Grik4 (Myc-DDK-tagged) - Mouse glutamate receptor, ionotropic, kainate 4 (Grik4)

Product Images

Circular map for MR226146

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Grik4
• Synonyms: 6330551K01Rik; GluK4; GluRgamma1; KA-1; KA1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR226146 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCCCGTGTCTCCGCTCCTCTGGTGCTGCTTCCTGCTTGGCTCTTGATGGTGGCCTGTAGCCCACACT
  CCCTGAGGATTGCTGCTATCTTGGATGACCCCATGGAGTGCAGCAGAGGGGAACGGCTCTCCATCACCCT
  GGCCAAGAACCGCATCAACCGCGCACCAGAGAGGCTGGGCAAGGCCAAGGTCGAAGTGGACATCTTTGAG
  CTTCTCAGAGACAGCGAGTACGAAACTGCAGAAACCATGTGTCAGATCCTCCCCAAGGGTGTGGTCGCTG
  TCCTGGGACCATCGTCCAGCCCAGCCTCCAGCTCCATCATCAGCAACATCTGTGGAGAGAAGGAGGTCCC
  TCATTTCAAGGTGGCCCCAGAGGAATTTGTCAGGTTCCAACTCCAGAGATTCACCACCCTGAACCTTCAC
  CCCAGCAACACCGACATCAGCGTGGCTGTGGCTGGGATCCTGAACTTCTTCAACTGCACCACCGCCTGCC
  TCATCTGTGCCAAAGCAGAATGCCTTTTAAACCTGGAGAAGCTGCTCCGACAATTCCTTATCTCCAAGGA
  CACACTCTCGGTCCGGATGTTGGACGACACACGGGACTCCACTCCACTCCTCAAGGAGATCCGGGATGAC
  AAGACAGCTACTATCATCATCCACGCCAACGCCTCCATGTCGCACACCATCCTCCTGAAGGCAGCTGAAC
  TGGGGATGGTGTCAGCCTATTACACATACATCTTCACCAATCTGGAGTTCTCCCTCCAGAGAATGGACAG
  CCTTGTGGATGATAGAGTCAACATCTTAGGATTTTCCATTTTCAACCAATCCCATGCATTCTTCCAAGAG
  TTCTCCCAGAGCCTCAACCAATCCTGGCAGGAGAACTGTGACCATGTGCCCTTCACCGGCCCTGCGTTGT
  CCTCAGCCCTGCTGTTTGACGCTGTCTATGCTGTGGTGACAGCGGTACAGGAACTGAACCGGAGCCAAGA
  GATTGGTGTGAAGCCCCTGTCCTGTGGCTCAGCCCAGATCTGGCAGCATGGCACCAGCCTCATGAACTAC
  CTTCGCATGGTAGAATTGGAAGGTCTTACCGGCCACATTGAATTCAACAGCAAAGGCCAGAGGTCCAACT
  ATGCCTTGAAAATCTTACAGTTCACCAGGAATGGGTTTCAGCAGATTGGCCAGTGGCATGTGGCAGAGGG
  CCTCAGCATGGACAGCCGCCTCTACGCCTCCAACATCTCTGACAGTCTCTTCAACACCACCCTGGTCGTC
  ACCACCATCCTGGAAAACCCATACTTAATGCTGAAGGGGAACCACCAGGAGATGGAAGGCAATGACCGAT
  ATGAGGGATTCTGTGTGGACATGCTCAAGGAGTTGGCTGAGATCCTACGGTTCAACTACAAGATCCGCCT
  TGTTGGGGATGGTGTTTACGGCGTACCTGAGGCCAACGGCACCTGGACAGGCATGGTTGGGGAGCTGATC
  GCTCGGAAAGCGGATCTGGCTGTGGCGGGCCTCACCATCACAGCGGAACGTGAGAAAGTGATCGATTTCT
  CCAAGCCATTCATGACGCTGGGAATTAGTATTCTTTACAGAGTTCATATGGGACGCAGACCGGGCTATTT
  CTCCTTCCTGGATCCCTTTTCTCCAGGAGTCTGGCTCTTCATGCTTCTAGCTTATCTGGCGGTCAGCTGT
  GTCCTCTTCCTGGTGGCTCGGTTGACTCCTTATGAGTGGTACAGTCCACATCCTTGTGCGCAGGGCCGGT
  GTAATCTCCTGGTCAACCAGTACTCCCTGGGCAACAGCCTCTGGTTTCCGGTTGGAGGCTTCATGCAGCA
  AGGCTCCACTATTGCCCCTCGAGCTTTGTCTACCCGCTGTGTCAGTGGTGTCTGGTGGGCATTCACGTTG
  ATCATCATCTCCTCCTACACGGCCAACCTGGCAGCCTTCCTGACTGTGCAGCGCATGGAGGTGCCCATTG
  AGTCTGTGGATGACCTGGCTGACCAGACTGCCATTGAGTATGGCACAATTCATGGAGGCTCCAGCATGAC
  CTTCTTCCAAAATTCCCGTTACCAGACCTACCAACGCATGTGGAACTACATGTACTCCAAGCAGCCGAGT
  GTGTTCGTGAAGAGCACGGAGGAGGGGATTGCCAGAGTGTTGAATTCCAATTACGCCTTCCTGCTGGAGT
  CTACCATGAATGAGTACTACCGGCAGCGAAACTGCAACCTCACTCAGATTGGGGGCCTGCTGGACACCAA
  GGGCTATGGGATTGGCATGCCAGTCGGCTCAGTGTTCCGGGATGAGTTCGACCTGGCCATTCTCCAGCTG
  CAGGAGAACAACCGCCTGGAAATTCTGAAGCGGAAGTGGTGGGAAGGGGGAAAATGTCCCAAGGAGGAGG
  ACCATCGAGCCAAAGGCCTGGGAATGGAGAATATTGGTGGAATCTTTGTGGTTCTTATTTGTGGCTTAAT
  AGTGGCCATTTTTATGGCTATGCTGGAGTTTTTGTGGACTCTCAGACACTCAGAAGCATCAGAGGTGTCT
  GTGTGTCAGGAGATGGTGACAGAACTGCGCAACATCATCCTGTGCCAGGACAATATCCATCCCAGGCGGC
  GGCGCTCTGGAGGCCTGCCACCCCAGCCCCCGGTCCTGGAGGAGCGCCGACCCAGAGGCACAGCGACGCT
  CAGCAATGGCAAGCTATGTGGCGCCGGGGAGCCAGACCAGCTGGCGCAGAGACTGGCACAGGAGGCCGCC
  CTAGTAGCTCGAGGCTGCACGCACATCCGCGTGTGCCCAGAGTGCCGCCGCTTCCAGGGCCTGCGGGCGC
  GACCGTCGCCAGCGCGCAGCGAGGAGAGCCTGGAGTGGGACAAGACCACCAACAGCAGCGAGCCCGAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR226146 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MPRVSAPLVLLPAWLLMVACSPHSLRIAAILDDPMECSRGERLSITLAKNRINRAPERLGKAKVEVDIFE
  LLRDSEYETAETMCQILPKGVVAVLGPSSSPASSSIISNICGEKEVPHFKVAPEEFVRFQLQRFTTLNLH
  PSNTDISVAVAGILNFFNCTTACLICAKAECLLNLEKLLRQFLISKDTLSVRMLDDTRDSTPLLKEIRDD
  KTATIIIHANASMSHTILLKAAELGMVSAYYTYIFTNLEFSLQRMDSLVDDRVNILGFSIFNQSHAFFQE
  FSQSLNQSWQENCDHVPFTGPALSSALLFDAVYAVVTAVQELNRSQEIGVKPLSCGSAQIWQHGTSLMNY
  LRMVELEGLTGHIEFNSKGQRSNYALKILQFTRNGFQQIGQWHVAEGLSMDSRLYASNISDSLFNTTLVV
  TTILENPYLMLKGNHQEMEGNDRYEGFCVDMLKELAEILRFNYKIRLVGDGVYGVPEANGTWTGMVGELI
  ARKADLAVAGLTITAEREKVIDFSKPFMTLGISILYRVHMGRRPGYFSFLDPFSPGVWLFMLLAYLAVSC
  VLFLVARLTPYEWYSPHPCAQGRCNLLVNQYSLGNSLWFPVGGFMQQGSTIAPRALSTRCVSGVWWAFTL
  IIISSYTANLAAFLTVQRMEVPIESVDDLADQTAIEYGTIHGGSSMTFFQNSRYQTYQRMWNYMYSKQPS
  VFVKSTEEGIARVLNSNYAFLLESTMNEYYRQRNCNLTQIGGLLDTKGYGIGMPVGSVFRDEFDLAILQL
  QENNRLEILKRKWWEGGKCPKEEDHRAKGLGMENIGGIFVVLICGLIVAIFMAMLEFLWTLRHSEASEVS
  VCQEMVTELRNIILCQDNIHPRRRRSGGLPPQPPVLEERRPRGTATLSNGKLCGAGEPDQLAQRLAQEAA
  LVARGCTHIRVCPECRRFQGLRARPSPARSEESLEWDKTTNSSEPE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_175481
• ORF Size: 2871 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_175481.1, NM_175481.2, NM_175481.3, NM_175481.4, NM_175481.5, NP_780690.2
• RefSeq Size: 3488 bp
• RefSeq ORF: 2871 bp
• Locus ID: 110637
• UniProt ID: Q8BMF5
• Cytogenetics: 9 23.8 cM
• MW: 107.3 kDa
• Gene Summary: Receptor for glutamate. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. The postsynaptic actions of Glu are mediated by a variety of receptors that are named according to their selective agonists. This receptor binds kainate > quisqualate > glutamate >> AMPA (By similarity).[UniProtKB/Swiss-Prot Function]