Comp (NM_016685) Mouse Tagged ORF Clone

CAT#: MR224430

Comp (Myc-DDK-tagged) - Mouse cartilage oligomeric matrix protein (Comp)

Product Images

Circular map for MR224430

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Comp
• Synonyms: TSP5
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR224430 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCCCCACTGCCTGCGTTCTAGTGCTCGCCCTGGCTATCCTGCGGGCGACAGGCCAGGGCCAGATCC
  CGCTGGGTGGAGACCTGGCCCCACAGATGCTGCGAGAACTTCAGGAGACTAATGCGGCGCTGCAAGACGT
  GAGAGAGCTGTTGCGACAGCAGGTCAAGGAGATCACCTTCCTGAAGAATACGGTGATGGAATGTGATGCT
  TGCGGAATGCAGCCCGCACGCACTCCAGGCCTGAGCGTGCGGCCAGTGCCGCTCTGCGCACCCGGCTCCT
  GCTTCCCCGGCGTAGTCTGCTCCGAGACAGCTACGGGCGCGCGCTGCGGCCCCTGCCCTCCTGGCTACAC
  CGGCAACGGCTCGCACTGCACCGACGTTAATGAGTGCAATGCTCACCCCTGTTTCCCGCGGGTGCGGTGC
  ATCAATACCAGCCCTGGCTTTCACTGCGAAGCCTGTCCCCCTGGGTTCAGCGGACCCACCCACGAGGGCG
  TGGGACTGACCTTCGCTAAGTCCAACAAACAAGTTTGCACGGATATTAATGAGTGTGAGACCGGGCAGCA
  CAATTGCGTTCCCAACTCCGTGTGCGTCAACACCCGGGGCTCCTTCCAGTGCGGCCCCTGCCAGCCCGGT
  TTCGTGGGCGACCAGACGTCAGGCTGCCAGCGGCGTGGGCAGCACTTCTGCCCCGATGGGTCACCCAGCC
  CGTGCCATGAGAAAGCAAACTGCGTCCTGGAGCGGGATGGCTCGAGGTCTTGCGTGTGTGCAGTCGGCTG
  GGCCGGCAACGGGCTCCTGTGCGGCCGCGACACGGACCTGGACGGTTTTCCTGACGAGAAGCTTCGCTGC
  TCAGAGCGCCAGTGTCGCAAGGACAACTGCGTGACGGTGCCCAATTCGGGGCAGGAGGATGTGGACCGGG
  ACGGCATCGGAGATGCTTGTGACCCGGATGCGGACGGGGATGGAGTCCCTAACGAGCAAGACAATTGCCC
  GCTGGTTCGAAACCCAGACCAGCGTAACTCGGACAGTGATAAGTGGGGAGATGCCTGCGACAACTGCCGG
  TCCAAGAAGAATGACGATCAGAAAGATACAGACCTGGATGGCCGGGGCGATGCCTGCGACGACGACATAG
  ATGGCGACGGAATACGAAATGTAGCTGACAACTGTCCCCGGGTGCCCAACTTTGACCAGAGTGACAGTGA
  TGGTGATGGTGTTGGGGATGCCTGTGACAACTGTCCCCAGAAAGATAACCCAGACCAGAGGGATGTGGAC
  CACGACTTTGTGGGTGATGCCTGTGATAGTGACCAAGACCAGGATGGGGATGGTCACCAGGACTCCCGGG
  ACAACTGCCCCACAGTACCCAACAGTGCCCAGCAGGACTCAGATCATGATGGCAAGGGCGATGCCTGTGA
  TGACGATGATGACAATGACGGAGTTCCTGATAGCCGGGACAACTGCCGCTTGGTGCCTAACCCTGGCCAA
  GAGGACAATGACCGGGATGGCGTGGGTGACGCGTGTCAGGGTGACTTCGATGCTGACAAGGTTATAGACA
  AGATCGATGTGTGCCCCGAGAACGCCGAGGTCACCCTCACCGACTTCAGGGCCTTCCAGACGGTTGTGTT
  GGACCCCGAGGGTGATGCGCAGATCGATCCCAACTGGGTGGTGCTCAATCAGGGAATGGAGATCGTTCAG
  ACCATGAACAGTGACCCTGGCCTGGCTGTGGGTTACACAGCCTTCAACGGCGTGGACTTCGAGGGCACAT
  TCCATGTAAACACCGCCACTGATGATGACTATGCTGGTTTCATCTTTGGCTACCAAGACAGCTCCAGTTT
  CTATGTAGTCATGTGGAAACAGATGGAGCAGACGTACTGGCAGGCCAATCCCTTCCGGGCTGTGGCTGAG
  CCAGGGATTCAGCTCAAGGCTGTCAAGTCCTCTACAGGTCCCGGGGAACAGCTCCGAAACGCACTTTGGC
  ACACGGGGGACACAGCATCCCAGGTGCGGCTGCTGTGGAAGGATCCTCGAAACGTGGGCTGGAAGGATAA
  AACATCCTACCGCTGGTTCCTGCAGCACCGGCCTCAAGTTGGCTACATCAGGGTGCGGTTCTATGAGGGT
  CCTGAGCTAGTAGCTGACAGCAATGTGGTGTTGGACACGGCCATGCGTGGTGGCCGCCTGGGTGTCTTCT
  GCTTCTCCCAAGAGAACATCATCTGGGCTAACCTGCGCTACCGTTGCAATGATACAATCCCTGAGGACTA
  CGAGAGTCACCGGCTGCAGAGAGTC
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR224430 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MGPTACVLVLALAILRATGQGQIPLGGDLAPQMLRELQETNAALQDVRELLRQQVKEITFLKNTVMECDA
  CGMQPARTPGLSVRPVPLCAPGSCFPGVVCSETATGARCGPCPPGYTGNGSHCTDVNECNAHPCFPRVRC
  INTSPGFHCEACPPGFSGPTHEGVGLTFAKSNKQVCTDINECETGQHNCVPNSVCVNTRGSFQCGPCQPG
  FVGDQTSGCQRRGQHFCPDGSPSPCHEKANCVLERDGSRSCVCAVGWAGNGLLCGRDTDLDGFPDEKLRC
  SERQCRKDNCVTVPNSGQEDVDRDGIGDACDPDADGDGVPNEQDNCPLVRNPDQRNSDSDKWGDACDNCR
  SKKNDDQKDTDLDGRGDACDDDIDGDGIRNVADNCPRVPNFDQSDSDGDGVGDACDNCPQKDNPDQRDVD
  HDFVGDACDSDQDQDGDGHQDSRDNCPTVPNSAQQDSDHDGKGDACDDDDDNDGVPDSRDNCRLVPNPGQ
  EDNDRDGVGDACQGDFDADKVIDKIDVCPENAEVTLTDFRAFQTVVLDPEGDAQIDPNWVVLNQGMEIVQ
  TMNSDPGLAVGYTAFNGVDFEGTFHVNTATDDDYAGFIFGYQDSSSFYVVMWKQMEQTYWQANPFRAVAE
  PGIQLKAVKSSTGPGEQLRNALWHTGDTASQVRLLWKDPRNVGWKDKTSYRWFLQHRPQVGYIRVRFYEG
  PELVADSNVVLDTAMRGGRLGVFCFSQENIIWANLRYRCNDTIPEDYESHRLQRV
  
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_016685
• ORF Size: 2268 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_016685.1, NM_016685.2, NP_057894.2
• RefSeq Size: 2447 bp
• RefSeq ORF: 2268 bp
• Locus ID: 12845
• UniProt ID: Q9R0G6
• Cytogenetics: 8 34.15 cM
• MW: 82.2 kDa
• Gene Summary: May play a role in the structural integrity of cartilage via its interaction with other extracellular matrix proteins such as the collagens and fibronectin. Can mediate the interaction of chondrocytes with the cartilage extracellular matrix through interaction with cell surface integrin receptors. Could play a role in the pathogenesis of osteoarthritis. Potent suppressor of apoptosis in both primary chondrocytes and transformed cells. Suppresses apoptosis by blocking the activation of caspase-3 and by inducing the IAP family of survival proteins (BIRC3, BIRC2, BIRC5 and XIAP). Essential for maintaining a vascular smooth muscle cells (VSMCs) contractile/differentiated phenotype under physiological and pathological stimuli. Maintains this phenotype of VSMCs by interacting with ITGA7 (By similarity).[UniProtKB/Swiss-Prot Function]