Palb2 (NM_001081238) Mouse Tagged ORF Clone

CAT#: MR223631

Palb2 (Myc-DDK-tagged) - Mouse partner and localizer of BRCA2 (Palb2)

Product Images

Circular map for MR223631

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Palb2
• Synonyms: BC066140
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR223631 representing NM_001081238
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAGAGCTTTCCGGGAAGCCCCTCAGCTATGCGGAGAAGGAAAAGTTGAAGGAAAAGCTAGCATTTT
  TGAAAAAGGAATACAGCAGGACACTTGCTCGACTTCAGCGTGCCAAAAGAGCTGAGAAGGCTAAGAACTC
  TAAGAAAGCCATAGAAGATGGTGTGCCCCAGCCAGAAGCTTCCTCACAGCTAAGCCACTCTGAATCTATA
  AATAAAGGCTTTCCTTGTGACACATTGCAAAGCAACCATCTGGATGAGGAGACTGGAGAAAACATCTCTC
  AGATACTTGATGTTGAACCTCAATCCTTTAACTGTAAACAAGGCAAAGAAGTATTACATACACCAAGAGC
  AGGTGACATTCAAGGACAACTTTTGCATAGCACCAGCAGCCCTGATGGCAAGAAAGAGCAGAACACGCTT
  CCGGGGACAACAAAGACGCCGTGGGAGAAGTCATCTGTTTCACAGGAGAAGGAAGATTATTTTGACACTA
  ATTCTCTGGCGCTCCTTGGTAAGCATCGAAAAGGGCAAGAATCAATCAGTAGGAAGAATTCTAGGACTCC
  CGTGAGTGAAAAGACTCACCTTTTAAGTCTCAGGTCTCAGATCCCTGACCCTCCAGCACTTGTTACAGGA
  ATTGGAGAGGGTATATTAATTCCGCCATCTGGCAAATCAGAAAGGGGAATTGATACACTTGTGAGAGGAA
  ATACTGTCTCCGCGGAGGCTGCAGTTCCTTCATGTACTGCGTCAAACAGCAATCACAGTCAGCACCTTGA
  GCATACACCTCCTAAAAGTGGCTGCAAAATTACTACTCAGGGCCCGGCTTCATCCACAAACCTGGTGGCA
  CAAGACCAAAAAATGACTATATTTACAGTAAACTCAGTAGTATATAAAGCTGTGCGTGCCCATGGTCAGC
  TGCCAGGAAGTCCCAATTCTTGTTCTGTAAATGATCTCACGCATAGTAACTTGCCAGCAAATAGTACCCC
  AAACTCTAAATCTTTAAAATCTCCCAGTAACACTGTTGATGAGAGAAATGAACCTCTTCAGGAAGATGAA
  ATTCTTGGTCCATCTAAGAACTTCAACCTGGCAGCAGTCTCTCCTCCTTCCACAGAAAGTCAAATACATT
  CTTGTACTATGCTTGAAGGCCTTCTGTTTCCTGCAGAATACTATGTTAGAACAACCCGTCGTATGTCAGA
  TTGTCAGAGAAAAATAGCTCTGGAAGCTGTAATTCAAAGTCATTTGGGTGTCAAAAAGAAAGAGCTTAAA
  AAGAAAACCAAAGCTACTAAGGCGGTCGTCCTCTCCAGTGAAGACACTGACCAGAGTGAAAGTGGCATGC
  TGGACACGAGCACGGGACAGTCCAGTTCAGGAAGCCTCTCTCAGAAACTGCTCTCACCAGCTGAGGTCAG
  CTCTCCTCCAGGACCTGCTGGAAAGGCCACCACACCGCCACCTGGTAGAGGACACAGAGGGAAACGAAAA
  TCAGCCCGCACCTCCACACTGGGTCACTGCCAGCTGCTTTTTCCTCCTTGTGCCGCACTGGCTGTTAACA
  GGTCCAAGGGCAAATTCACCAAGCATAAATGTCAGAACAGAGGCGTGGTTATTCATGACTTTGAGTTACC
  TGATGAAGACTTTGGGCTTCTTAAACTTGAAAAATTGAAGTCCTGCTCGGAAAAACTGATTGAGTCTCCT
  GACTCAAAAAACTGTGGTGAGAGGCTTCCTAGAGAAGGAAACCATGCTGCTCTGGAGGAACTGCAAAGAG
  ATTCGGAGACGGAGGGCTTGGAAGAGGAGCTCACTGTTCCACCAGGAGAAGCGTACCGTCCAGGGCCAAC
  CCTGAGAAGGCAGCCAGGGAGCAAGGACCTTTCTTCATCCATAGTGCTTTTCACTCCTGCAGACACTGCT
  GCGCCTAACGACAGTGGCAGGCCTCCTCCCTCCCTGTGCTCACCTGCTTTCCCCATCTTAGGCATGACTC
  CAGCTCTTGGCTCCCAAGCAGCCGGTGAGACTCTATCTACTGAAGCTGCACAGCCTTGCTCTACATCCCA
  ACCTCCTCTCTTGGGAGACACAAACAGTCTTGTCAATAACAGTAAACAGTGCAACAGTTCAGCCTGCTCA
  CCAAAACCGGACACCAACCTGCAAGCGTCAGGTAGGCAAGGACAACCTGCCTGTGACAGTGACTCTGGCC
  CCCAAGCAACACCTCTACCTGTTGAGTCATTCACTTTCAGAGAAAATCAGCTTTGTGGAAATGCATGCCT
  CGAGTTACATGAACATTCCACTGAACAGACTGAAACTGCAGATCGCCCTGCTTGTGACAATCTAAACCCA
  GGAAACCTACAGTTGGTTTCAGAGTTAAAGAATCCTTCCAGTTCCTGCTCTGTGGATGTGAGCGCCATGT
  GGTGGGAAAGAGCTGGTGCTAAGGAGCCATGTATCGTAACTGCTTGTGAAGATGTAGTTTCTCTTTGGAA
  ACCCTTGAATTCTCTGCAGTGGGAGAAAGTTCATACCTGGCACTTCACAGAGGTTCCAGTATTACAAATA
  GTTCCAGTGCCTGATGTTTACAATCTTATATGTGTAGCTTTGGGAAGTTTGGAAATCAGAGAAATCAGGG
  CGTTGCTGTGCTCTTCTGGTGATGACAGTGAAAAGCAAGTCCTCCTGAAATCTGGAGATATAAAAGCTAT
  GCTTGGCCTGACAAAGCGGAGGCTAGTTAGTAGCACTGGGACCTTTTGCAATCAACAAATACAAATCATG
  ACATTTGCTGACGACGGAAGCAGCAAAGATGAACAGCTTTTGATGCCTCCTGATGAGACTGTACTGACTT
  TTGCTGAAGTCCAAGGGACGCAGGAAGCTCTGCTTGGTACTACCACTGTGAACAGCATTGTGATCTGGAA
  TTTAAAAACCGGCCAGCTCCTGAAGAAGATGCACATTGATGACTCCTACCAGGCTTCGGTCTGTCACGGA
  GCCTATTCTGAGAAGGGGCTCCTGTTTGTTGTTGTGAGTCAACCTTGTGCCAAAGAGAGCCAGGCCCTTG
  GAAGCCCTGTGTTCCAGCTGCTGGTGATTAACCCTAAGACGGCCCAGAGTGTGGGTGTTCTGCTGTGTTC
  CCTTCCTCAGGGGCAGGCTGGAAGGTTCCTGGAAGGGGATGTGAAAGATCATGTCGCAGCAGCAGTCCTG
  ACTTCTGGGACAATTGCCATTTGGGATTTGCTTCTGGGTCACTGCACGGCTCTCCTCCCACCTGTCTCTG
  ACCAGAGTTGGTCTTTGGTTAAATGGTCGGGTACAGATTCTCACTTGCTAGCTGGACAAAAGGATGGAAA
  TATATTTATATACCGATACTTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR223631 representing NM_001081238
  Red=Cloning site Green=Tags(s)
  
  MEELSGKPLSYAEKEKLKEKLAFLKKEYSRTLARLQRAKRAEKAKNSKKAIEDGVPQPEASSQLSHSESI
  NKGFPCDTLQSNHLDEETGENISQILDVEPQSFNCKQGKEVLHTPRAGDIQGQLLHSTSSPDGKKEQNTL
  PGTTKTPWEKSSVSQEKEDYFDTNSLALLGKHRKGQESISRKNSRTPVSEKTHLLSLRSQIPDPPALVTG
  IGEGILIPPSGKSERGIDTLVRGNTVSAEAAVPSCTASNSNHSQHLEHTPPKSGCKITTQGPASSTNLVA
  QDQKMTIFTVNSVVYKAVRAHGQLPGSPNSCSVNDLTHSNLPANSTPNSKSLKSPSNTVDERNEPLQEDE
  ILGPSKNFNLAAVSPPSTESQIHSCTMLEGLLFPAEYYVRTTRRMSDCQRKIALEAVIQSHLGVKKKELK
  KKTKATKAVVLSSEDTDQSESGMLDTSTGQSSSGSLSQKLLSPAEVSSPPGPAGKATTPPPGRGHRGKRK
  SARTSTLGHCQLLFPPCAALAVNRSKGKFTKHKCQNRGVVIHDFELPDEDFGLLKLEKLKSCSEKLIESP
  DSKNCGERLPREGNHAALEELQRDSETEGLEEELTVPPGEAYRPGPTLRRQPGSKDLSSSIVLFTPADTA
  APNDSGRPPPSLCSPAFPILGMTPALGSQAAGETLSTEAAQPCSTSQPPLLGDTNSLVNNSKQCNSSACS
  PKPDTNLQASGRQGQPACDSDSGPQATPLPVESFTFRENQLCGNACLELHEHSTEQTETADRPACDNLNP
  GNLQLVSELKNPSSSCSVDVSAMWWERAGAKEPCIVTACEDVVSLWKPLNSLQWEKVHTWHFTEVPVLQI
  VPVPDVYNLICVALGSLEIREIRALLCSSGDDSEKQVLLKSGDIKAMLGLTKRRLVSSTGTFCNQQIQIM
  TFADDGSSKDEQLLMPPDETVLTFAEVQGTQEALLGTTTVNSIVIWNLKTGQLLKKMHIDDSYQASVCHG
  AYSEKGLLFVVVSQPCAKESQALGSPVFQLLVINPKTAQSVGVLLCSLPQGQAGRFLEGDVKDHVAAAVL
  TSGTIAIWDLLLGHCTALLPPVSDQSWSLVKWSGTDSHLLAGQKDGNIFIYRYF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001081238
• ORF Size: 3312 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001081238.2, NP_001074707.1
• RefSeq Size: 3747 bp
• RefSeq ORF: 3315 bp
• Locus ID: 233826
• UniProt ID: Q3U0P1
• Cytogenetics: 7 F2
• MW: 119.5 kDa
• Gene Summary: Plays a critical role in homologous recombination repair (HRR) through its ability to recruit BRCA2 and RAD51 to DNA breaks. Strongly stimulates the DNA strand-invasion activity of RAD51, stabilizes the nucleoprotein filament against a disruptive BRC3-BRC4 polypeptide and helps RAD51 to overcome the suppressive effect of replication protein A (RPA). Functionally cooperates with RAD51AP1 in promoting of D-loop formation by RAD51. Serves as the molecular scaffold in the formation of the BRCA1-PALB2-BRCA2 complex which is essential for homologous recombination. Via its WD repeats is proposed to scaffold a HR complex containing RAD51C and BRCA2 which is thought to play a role in HR-mediated DNA repair. Essential partner of BRCA2 that promotes the localization and stability of BRCA2. Also enables its recombinational repair and checkpoint functions of BRCA2. May act by promoting stable association of BRCA2 with nuclear structures, allowing BRCA2 to escape the effects of proteasome-mediated degradation. Binds DNA with high affinity for D loop, which comprises single-stranded, double-stranded and branched DNA structures. May play a role in the extension step after strand invasion at replication-dependent DNA double-strand breaks; together with BRCA2 is involved in both POLH localization at collapsed replication forks and DNA polymerization activity (By similarity).[UniProtKB/Swiss-Prot Function]