Slc27a6 (NM_001081072) Mouse Tagged ORF Clone

CAT#: MR221519

Slc27a6 (Myc-DDK-tagged) - Mouse solute carrier family 27 (fatty acid transporter), member 6 (Slc27a6)

Product Images

Circular map for MR221519

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Slc27a6
• Synonyms: 4732438L20Rik; FACVL2; FATP6; VLCS-H1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR221519 representing NM_001081072
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTCCTGTCATGGCTCACAGGATTGGGGGCTGGACTGCTCTCCCTGCATTTCCTGCAGAAACTTCTGT
  TCCCGTATTTCTGGGATGATTTCTGGTACTTGCTGAAGGTGGTGCGCTACGGAATTCAGATGGAGATGTA
  CAAACTGAGGGGGGAGCTGGTCACGGTGCTGGATAAGTTCCTGAGCCACACCAGGAAGCAACCACGGAAA
  GCCTTCATCATTTATGAGGGGGACGTCTACACCTACGAGGACGTGGACAAGAGGAGTAACAGAATAGCCC
  ACGCTCTCCTGAACCACTCCTCGCTGAAAAGGGGGGACGTTGTGGCTTTGTTGATGAGCAACGAGCCCGA
  CTTCGTTCATGTGTGGTTTGGCCTGGCTAAACTGGGCTGCGTGGTGGCCTTCCTCAACTCCAACCTTCGC
  TTCGATTCCCTCCTACACTGCATCAACACCTGTGAACCCACTGCCGTGGTGGTGGGCGGAGATTTGCTTG
  GAAGCATAGAAGAAATACTTCCCAGTCTCCCAAAACACGTCAGAGTTTGGGGAATGAAGGATTCTGTTCC
  AGAAGGTATAGATTCACTCCAAGAAAAGCTGAGTCTCGCCTCTGATGAGCCAGTACCACCAAGCCATCAT
  GTCACCTCAAGCCTCAAGTCTACTTGCCTCTACATTTTTACCTCTGGAACAACAGGTCTACCCAAAGCAG
  CTGTGATTAGCCAGTTGCAAGTGTTGAAGGGATCTGTTGGGCTATGGGCGTTTGGCTGCACAGCTGATGA
  CATCATTTATATAACCCTCCCTCTGTACCACAGCTCAGGGTCTCTCCTTGGAATTGGTGGATGTGTTGAG
  TTGGGCGCCACGTGTGTGCTAAAGAAGAAATTCTCCGCAAGCCAGTTTTGGAATGATTGCAAGAAGTACA
  ACGTGACTGTGTTTCAGTACATTGGGGAACTTTGCCGTTATCTGTGCAAACAGCCTCAGAGAGAAGGGGA
  AAAGGACCATCGGGTACGTTTGGCAGTTGGAAACGGTTTGAGCAGTGATGTGTGGAGACAATTTTTAGAC
  AGATTTGGAAATATTAAAATGTGTGAACTGTACGGAGCTACCGAAGGAAACATAGTCTTCATGAACCATA
  CTGGGAAGATTGGATCTGTCGGGAGAGCAAATTTCTTCTACAGTCTCTTTTTCTCCTTTGAGTTGATCAA
  GTATGACTTTCAGAAAGATGAACCATGGCGGAACGGACAAGGCTGGTGCTCCTGTGTGAGAAAAGGAGAA
  CCTGGTCTCCTCATTTCTCGTGTGAATAAGAAGAATCCCTTCTTCGGCTACGCTGGCTCGGACACGCACA
  CGAAAAGCAAGCTGCTCTTTGATGTGTTTCGGAAGGGAGACGTGTACTTCAACACCGGAGACCTAATGTT
  CCAAGACCAGGAGAACTTCGTTTATTTTTGGGACCGTCTTGGAGACACTTTCAGGTGGAAAGGAGAAAAC
  GTCGCAACCACAGAGGTCGCTGATGTGCTTGGGAGGTTGGACTTCATCCAGGAAGCAAACGTGTACGGTG
  TGCGTGTGCCAGGTTATGAAGGAAAAGCAGGAATGACTTCTGTTATCCTAAAACCAAACAAGTCTTTAGA
  CTTAGAAAAAATGTACAACCAAGTGGTGACATCTCTGCCAGCTTACGCCTGCCCACTGTTTTTAAGAATT
  CAGGACAAAATGGAAACTACAGGGACATTCAAGCTAAAGAAGCTACAACTGGTGGAAGAAGGATTTGATC
  CGCTGAAGATTTCTGACCCGCTTTACTTTATGGATAACTTGAAAAAATCTTATGTCCCATTGACCGAAGA
  GATTTATAATCAGATAATGTCAGAGGAAGTTAAACTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR221519 representing NM_001081072
  Red=Cloning site Green=Tags(s)
  
  MLLSWLTGLGAGLLSLHFLQKLLFPYFWDDFWYLLKVVRYGIQMEMYKLRGELVTVLDKFLSHTRKQPRK
  AFIIYEGDVYTYEDVDKRSNRIAHALLNHSSLKRGDVVALLMSNEPDFVHVWFGLAKLGCVVAFLNSNLR
  FDSLLHCINTCEPTAVVVGGDLLGSIEEILPSLPKHVRVWGMKDSVPEGIDSLQEKLSLASDEPVPPSHH
  VTSSLKSTCLYIFTSGTTGLPKAAVISQLQVLKGSVGLWAFGCTADDIIYITLPLYHSSGSLLGIGGCVE
  LGATCVLKKKFSASQFWNDCKKYNVTVFQYIGELCRYLCKQPQREGEKDHRVRLAVGNGLSSDVWRQFLD
  RFGNIKMCELYGATEGNIVFMNHTGKIGSVGRANFFYSLFFSFELIKYDFQKDEPWRNGQGWCSCVRKGE
  PGLLISRVNKKNPFFGYAGSDTHTKSKLLFDVFRKGDVYFNTGDLMFQDQENFVYFWDRLGDTFRWKGEN
  VATTEVADVLGRLDFIQEANVYGVRVPGYEGKAGMTSVILKPNKSLDLEKMYNQVVTSLPAYACPLFLRI
  QDKMETTGTFKLKKLQLVEEGFDPLKISDPLYFMDNLKKSYVPLTEEIYNQIMSEEVKL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001081072
• ORF Size: 1857 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001081072.1, NP_001074541.1
• RefSeq Size: 2598 bp
• RefSeq ORF: 1860 bp
• Locus ID: 225579
• UniProt ID: E9Q9W4
• Cytogenetics: 18 D3
• MW: 70.2 kDa
• Gene Summary: Involved in translocation of long-chain fatty acids (LFCA) across the plasma membrane (PubMed:15699031). Thought to function as the predominant fatty acid protein transporter in heart (By similarity). Has acyl-CoA ligase activity for long-chain and very-long-chain fatty acids (VLCFAs) (PubMed:15699031).[UniProtKB/Swiss-Prot Function]